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Comparative Effect of Ethyl Urethane and Cycloheximide in Lepidium sativum L. Seed Germination and Radicle Growth

  • O. Viman
  • K. Balla
  • L. HolonecEmail author
  • M. Tămaș
  • D. L. Dumitrașcu
  • V. Șandor
  • L. Nedelcu
Conference paper
Part of the IFMBE Proceedings book series (IFMBE, volume 71)

Abstract

The seed germination test of Lepidium sativum L. offers intrinsic data obtained within this complex process and details on the mechanisms of action of two pharmacologically distinct substances, ethyl urethane, a general anesthetic and cycloheximide, an inhibitor of protein biosynthesis in eukaryotes. The rate of germination of the species gives the test efficiency and the possibility of multiple experimental versions at identical time periods. Testing was carried out in aqueous media consisting of filter paper soaked with water or aqueous solutions of the two substances. It was evaluated the frequency of seed germination and the length of the radicle at 24 and 48 h after exposure. Comparison of results was made by nonparametric and parametric statistical tests to reject the null hypothesis at p < 0.05. Germination in the control group was over 90% and the length of rootlets was over 8 mm at 48 h. Ethyl urethane 1% delayed the germination, but it reached in 48 h at values close to those of the control group. The length of the radicle was significantly reduced, less than 3 mm. At this concentration, ethyl urethane clearly, but reversibly, inhibits germination. The germination is almost blocked at concentrations of 10% ethyl urethane, the effect being irreversible and the length of rootlets is at the limit of measurement. Cycloheximide 0.01% reduces to a greater extent than ethyl urethane 1% the germination frequency and length of rootlets; the action is irreversible. Inhibitory effects on germination of ethyl urethane and cycloheximide, substances with different pharmacological targets, show the importance of the general processes of cell excitability and the participation of specific proteins in the cascade phenomenon of germination in plants.

Keywords

Germination Lepidium sativum L. Ethyl urethane Cycloheximide 

Notes

Acknowledgements

This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • O. Viman
    • 1
  • K. Balla
    • 2
  • L. Holonec
    • 1
    Email author
  • M. Tămaș
    • 2
  • D. L. Dumitrașcu
    • 2
  • V. Șandor
    • 2
  • L. Nedelcu
    • 3
  1. 1.University of Agricultural Sciences and Veterinary MedicineCluj-NapocaRomania
  2. 2.“Iuliu Hațieganu” University of Medicine and PharmacyCluj-NapocaRomania
  3. 3.University “Transilvania”BrașovRomania

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