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Quantitation of mRNA Transcripts and Proteins Using the BD Rhapsody™ Single-Cell Analysis System

  • Eleen Y. ShumEmail author
  • Elisabeth M. Walczak
  • Christina Chang
  • H. Christina Fan
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1129)

Abstract

In this review, we describe the BD Rhapsody™ Single-Cell Analysis System, a platform that allows high-throughput capture of nucleic acids from single cells using a simple cartridge workflow and a multitier barcoding system. The resulting captured information can be used to generate various types of next-generation sequencing (NGS) libraries, including whole transcriptome analysis for discovery biology and targeted RNA analysis for high sensitivity transcript detection. The BD Rhapsody system can be used with emerging applications, such as BD™ AbSeq assays, to profile gene expression in both mRNA and protein level to provide ultra-high resolution analysis of single cells.

Keywords

Single cells Microwell array Barcoded beads Magnetic beads Next-generation sequencing Molecular barcoding Unique molecular identifiers UMI Transcriptome Targeted RNA-seq Antibody 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Eleen Y. Shum
    • 1
    Email author
  • Elisabeth M. Walczak
    • 1
  • Christina Chang
    • 1
  • H. Christina Fan
    • 1
  1. 1.BD BiosciencesSan JoseUSA

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