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On-Site MinION Sequencing

  • Lucky R. RuntuweneEmail author
  • Josef S. B. Tuda
  • Arthur E. Mongan
  • Yutaka SuzukiEmail author
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1129)

Abstract

DNA sequencing has reached an unprecedented level with the advent of Oxford Nanopore Technologies’ MinION. The low equipment investment, ease of library preparation, small size, and powered only by a laptop computer enable the portability for on-site sequencing. MinION has had its role in clinical, biosecurity, and environmental fields. Here, we describe the many facets of on-site sequencing with MinION. First, we will present some field works using MinION. We will discuss the requirements for targeted or whole genome sequencing and the challenges faced by each technique. We will also elaborate the bioinformatics procedures available for data analysis in the field. MinION has greatly changed the way we do sequencing by bringing the sequencer closer to the biodiversity. Although numerous limitations exist for MinION to be truly portable, improvements of procedures and equipment will enhance MinION’s role in the field.

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  1. 1.Graduate School of Frontier SciencesThe University of TokyoKashiwaJapan
  2. 2.Faculty of MedicineSam Ratulangi UniversityManadoIndonesia

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