Characterization of Arbuscular Mycorrhizal Communities in Roots of Vineyard Plants
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We developed a new protocol to study arbuscular mycorrhizal fungal (AMF) communities in Bordeaux vineyards in a standardized way, in order to compare results obtained over years and between locations. To this end, we first used grapevine root samples instead of soil samples to avoid AMF spores or hyphae interacting with cover plants. We next increased the number of grapevine samples to obtain more representative coverage of AMF communities while decreasing variability intra-vineyard, especially for the larger parcels. In addition, we adapted the DNA extraction protocol dedicated to soil samples to grapevine roots, as a way to increase the yield and the purity of samples. These features, coupled to the choice of the LSU-D2 region as the molecular marker for high-throughput sequencing (MiSeq® technology), further allowed us to assess the AMF populations of Bordeaux vineyards.
The authors acknowledge the financial support provided by the Bureau Interprofessionnel des Vins de Bourgogne (MYCOVITI Project), the Conseil Interprofessionel du Vin de Bordeaux (project 41971/41975), the Plant Health and Environment department from INRA and funding bodies within the H2020 ERA-net project, CORE Organic Cofund, and with cofunds from the European Commission (BIOVINE project).
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