Applications of Polyhydroxyalkanoates and Their Metabolites as Drug Carriers

  • Vipin Chandra Kalia
  • Subhasree Ray
  • Sanjay K. S. Patel
  • Mamtesh Singh
  • Gajendra Pratap Singh


Drug delivery systems based on biodegradable biomaterials have proved attractive to improve their efficacy. Biopolymers such as Polyhydroxyalkanoates (PHAs) have the potential to be used as materials for preparing nanoparticles as drug carriers. This review describes the recent developments in drug delivery systems based on PHAs derived from microbial fermentations. Various strategies for the synthesis of microspheres, encapsulations, nano-constructs, have been presented, with their potential applications in medical domain.


Polyhydroxyalkanoates Nanoparticles Drug carriers Bacteria 



This work was supported by Brain Pool grant (NRF-2018H1D3A2001746) by National Research Foundation of Korea (NRF) to work at Konkuk University.


  1. Alvarez-Lorenzo C, Concheiro A (2013) From drug dosage forms to intelligent drug-delivery systems: a change of paradigm. In: Alvarez-Lorenzo C, Concheiro A (eds) Smart materials for drug delivery. RSC Smart Mater Drug Deliv 1:1–32.
  2. Baran ET, Ozer N, Hasirci V (2002) Poly (hydroxybutyrate-co-hydroxyvalerate) nanocapsules as enzyme carriers for cancer therapy: an in vitro study. J Microencapsul 19:363–376. CrossRefPubMedGoogle Scholar
  3. Brasky TM, Cohn DE, Bernardo BM (2016) Aspirin and endometrial cancer risk. Gynecol Oncol Rep 17:1–2. CrossRefPubMedPubMedCentralGoogle Scholar
  4. Danhier F, Ansorena E, Silva JM, Coco R, Le Breton A, Préat V (2012) PLGA-based nanoparticles: an overview of biomedical applications. J Control Release 161:505–522. CrossRefPubMedGoogle Scholar
  5. Des Rieux A, Fievez V, Garinot M, Schneider YJ, Preat V (2006) Nanoparticles as potential oral delivery systems of proteins and vaccines: a mechanistic approach. J Control Release 116:1–27. CrossRefPubMedGoogle Scholar
  6. Francesko A, Tzanov T (2010) Chitin, chitosan and derivatives for wound healing and tissue engineering. In: Nyanhongo G, Steiner W, Gubitz GM (eds) Biofunctionalization of polymers and their applications. Adv Biochem Eng Biotechnol. Springer, Berlin. 125:1–27.
  7. Gürsel I, Korkusuz F, Türesin F, Alaeddinoǧlu NG, Hasırcı V (2001) In vivo application of biodegradable controlled antibiotic release systems for the treatment of implant-related osteomyelitis. Biomaterials 22:73–80. CrossRefPubMedGoogle Scholar
  8. Hägg M, Berndtsson M, Mandic A, Zhou R, Shoshan MC, Linder S (2004) Induction of endoplasmic reticulum stress by ellipticine plant alkaloids. Mol Cancer Ther 3:489–497PubMedGoogle Scholar
  9. Kalia VC, Patel SKS, Kang YC, Lee JK (2019) Quorum sensing inhibitors as antipathogens: biotechnological applications. Biotechnol. Adv. 37:68–90.
  10. Kamaly N, Yameen B, Wu J, Farokhzad OC (2016) Degradable controlled-release polymers and polymeric nanoparticles: mechanisms of controlling drug release. Chem Rev 116:2602–2663. CrossRefPubMedPubMedCentralGoogle Scholar
  11. Khang G, Kim SW, Cho JC, Rhee JM, Yoon SC, Lee HB (2001) Preparation and characterization of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) microspheres for the sustained release of 5-fluorouracil. Bio-med Mater Eng 11:89–103Google Scholar
  12. Kılıçay E, Demirbilek M, Türk M, Güven E, Hazer B, Denkbas EB (2011) Preparation and characterization of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHX) based nanoparticles for targeted cancer therapy. Eur J Pharm Sci 44:310–320. CrossRefPubMedGoogle Scholar
  13. Kim HN, Lee J, Kim HY, Kim YR (2009) Enzymatic synthesis of a drug delivery system based on polyhydroxyalkanoate-protein block copolymers. Chem Commun (Camb) 14:7104–7106. CrossRefGoogle Scholar
  14. Köse GT, Ber S, Korkusuz F, Hasirci V (2003) Poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) based tissue engineering matrices. J Mater Sci Mater Med 14:121–126. CrossRefPubMedGoogle Scholar
  15. Kumar P, Patel SKS, Lee JK, Kalia VC (2013) Extending the limits of Bacillus for novel biotechnological applications. Biotechnol Adv 31:1543–1561. CrossRefGoogle Scholar
  16. Kumar P, Singh M, Mehariya S, Patel SKS, Lee JK, Kalia VC (2014) Ecobiotechnological approach for exploiting the abilities of Bacillus to produce co-polymer of polyhydroxyalkanoate. Indian J Microbiol 54:151–157. CrossRefPubMedPubMedCentralGoogle Scholar
  17. Kumar P, Ray S, Patel SKS, Lee JK, Kalia VC (2015) Bioconversion of crude glycerol to polyhydroxyalkanoate by Bacillus thuringiensis under non-limiting nitrogen conditions. Int J Biol Macromol 78:9–16. CrossRefPubMedGoogle Scholar
  18. Kumar A, Kim I-W, Patel SKS, Lee J-K (2018a) Synthesis of protein-inorganic nanohybrids with improved catalytic properties using co (PO ). Indian J Microbiol 58:100–104. CrossRefPubMedGoogle Scholar
  19. Kumar A, Park GD, Patel SKS, Kondaveeti S, Otari S, Anwar MZ, Kalia VC, Singh Y, Kim SC, Cho BK, Sohn JH, Kim DR, Kang YC, Lee JK (2018b) SiO microparticles with carbon nanotube-derived mesopores as an efficient support for enzyme immobilization. Chem Eng J. CrossRefGoogle Scholar
  20. Li H, Chang J (2005) Preparation, characterization and in vitro release of gentamicin from PHBV/wollastonite composite microspheres. J Control Release 107:463–473. CrossRefPubMedGoogle Scholar
  21. Li Z, Loh XJ (2017) Recent advances of using polyhydroxyalkanoate-based nanovehicles as therapeutic delivery carriers. WIREs Nanomed Nanobiotechnol 9(3). Google Scholar
  22. Li XT, Zhang Y, Chen GQ (2008) Nano fibrous polyhydroxyalkanoate matrices as cell growth supporting materials. Biomaterials 29:3720–3728. CrossRefPubMedGoogle Scholar
  23. Liu Y, Shipton MK, Ryan J, Kaufman ED, Franzen S, Feldheim DL (2007) Synthesis, stability, and cellular internalization of gold nanoparticles containing mixed peptide− poly (ethylene glycol) monolayers. Anal Chem 79:2221–2229. CrossRefPubMedGoogle Scholar
  24. Lionzo MIZ, Re MI, Guterres SS, Pohlmann AR (2007) Microparticles prepared with poly(hydroxybutyrate-co-hydroxyvalerate) and poly(epsiloncaprolactone) blends to control the release of a drug model. J Microencapsul 24:175–186. CrossRefPubMedGoogle Scholar
  25. Lou Y, Zhu J (2016) Carboxylic acid nonsteroidal anti-inflammatory drugs (NSAIDs). In: Lamberth C, Dinges J (eds) Bioactive carboxylic compound classes: pharmaceuticals and agrochemicals. Wiley-VCH Verlag GmbH & Co. KGaA, Weinhein. CrossRefGoogle Scholar
  26. Lu XY, Ciraolo E, Stefenia R, Chen GQ, Zhang Y, Hirsch E (2011) Sustained release of PI3K inhibitor from PHA nanoparticles and in vitro growth inhibition of cancer cell lines. App Microbiol Biotechnol 89:1423–1433. CrossRefGoogle Scholar
  27. Mascolo DD, Basnett P, Palange AL, Francardi M, Roy I, Decuzzi P (2016) Tuning core hydrophobicity of spherical polymeric nanoconstructs for docetaxel delivery. Polym Int 65:741–746. CrossRefGoogle Scholar
  28. Masood F, Chen P, Yasin T, Fatima N, Hasan F, Hameed A (2012) Encapsulation of Ellipticine in poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) based nanoparticles and its in vitro application. Mater Sci Eng C 33:1054–1060. CrossRefGoogle Scholar
  29. Masood F, Chen P, Yasin T, Fatima N, Hasan F, Hameed A (2013a) Encapsulation of ellipticine in poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) based nanoparticles and its in vitro application. Mater Sci Eng 33:1054–1060. CrossRefGoogle Scholar
  30. Masood F, Chen P, Yasin T, Hasan F, Ahmad B, Hameed A (2013b) Synthesis of poly-(3-hydroxybutyrate-co-12 mol% 3-hydroxyvalerate) by Bacillus cereus FB11: its characterization and application as a drug carrier. J Mater Sci Mater Med 24:1927–1937. CrossRefPubMedGoogle Scholar
  31. Mittal G, Sahana DK, Bhardwaj V, Kumar MNVR (2007) Estradiol loaded PLGA nanoparticles for oral administration: effect of polymer molecular weight and copolymer composition on release behavior in vitro and in vivo. J Control Release 119:77–85. CrossRefPubMedGoogle Scholar
  32. Murueva AV, Shishatskaya EI, Kuzmina AM, Volova TG, Sinskey AJ (2013) Microparticles prepared from biodegradable polyhydroxyalkanoates as matrix for encapsulation of cytostatic drug. J Mater Sci Mater Med 24:1905–1915. CrossRefPubMedGoogle Scholar
  33. Murueva AV, Shershneva AM, Shishatskaya EI, Volova TG (2014) The use of polymeric microcarriers loaded with anti-inflammatory substances in the therapy of experimental skin wounds. Bull Exp Biol Med 157:597–602. CrossRefPubMedGoogle Scholar
  34. Nigmatullin R, Thomas P, Lukasiewicz B, Puthussery H, Roy I (2015) Polyhydroxyalkanoates, a family of natural polymers, and their applications in drug delivery. J Chem Technol Biotechnol 90:1209–1221. CrossRefGoogle Scholar
  35. Otari SV, Patel SKS, Kim S-Y, Haw JR, Kalia VC, Kim I-W, Lee J-K (2018) Copper ferrite magnetic nanoparticles for the immobilization of enzyme. Indian J Microbiol. CrossRefGoogle Scholar
  36. O’Connor S, Szwej E, Nikodinovic-Runic J, O’Connor A, Byrne AT, Devocelle M, O’Donovan N, Gallagher WM, Babu R, Kenny STM, Zinn M, Zulian QR, O’Conner KE (2013) The anti-cancer activity of a cationic anti-microbial peptide derived from monomers of polyhydroxyalkanoate. Biomaterials 34:2710–2718. CrossRefPubMedGoogle Scholar
  37. Pacheco DP, Amaral MH, Reis RL, Marques AP, Correlo VM (2015) Development of an injectable PHBV microparticles-GG hydrogel hybrid system for regenerative medicine. Int J Pharm 478:398–408. CrossRefPubMedGoogle Scholar
  38. Panyam J, Labhasetwar V (2004) Sustained cytoplasmic delivery of drugs with intracellular receptors using biodegradable nanoparticles. MolPharm 1:77–84. CrossRefGoogle Scholar
  39. Patel SKS, Singh M, Kalia VC (2011) Hydrogen and polyhydroxybutyrate producing abilities of Bacillus spp. from glucose in two stage system. Indian J Microbiol 51:418–423. CrossRefPubMedPubMedCentralGoogle Scholar
  40. Patel SKS, Singh M, Kumar P, Purohit HJ, Kalia VC (2012) Exploitation of defined bacterial cultures for production of hydrogen and polyhydroxybutyrate from pea-shells. Biomass Bioenergy 36:218–225. CrossRefGoogle Scholar
  41. Patel SKS, Kumar P, Singh S, Lee JK, Kalia VC (2015a) Integrative approach for hydrogen and polyhydroxybutyrate production. In: Kalia VC (ed) Microbial factories: waste treatment. Springer, New Delhi, pp 73–85. CrossRefGoogle Scholar
  42. Patel SKS, Kumar P, Singh S, Lee JK, Kalia VC (2015b) Integrative approach to produce hydrogen and polyhydroxybutyrate from biowaste using defined bacterial cultures. Bioresour Technol 176:136–141. CrossRefPubMedGoogle Scholar
  43. Patel SKS, Lee JK, Kalia VC (2016a) Integrative approach for producing hydrogen and polyhydroxyalkanoate from mixed wastes of biological origin. Indian J Microbiol 56:293–300. CrossRefPubMedPubMedCentralGoogle Scholar
  44. Patel SKS, Choi SH, Kang YC, Lee J-K (2016b) Large-scale aerosol-assisted synthesis of biofriendly Fe O yolk-shell particles: a promising support for enzyme immobilization. Nanoscale 8:6728–6738. CrossRefPubMedGoogle Scholar
  45. Patel SKS, Otari SV, Kang YC, Lee JK (2017a) Protein-inorganic hybrid system for efficient his-tagged enzymes immobilization and its application in L-xylulose production. RSC Adv 7:3488–3494. CrossRefGoogle Scholar
  46. Patel SKS, Choi SH, Kang YC, Lee J-K (2017b) Eco-friendly composite of Fe O -reduced graphene oxide particles for efficient enzyme immobilization. ACS Appl Mater Interfaces 9:2213–2222. CrossRefPubMedGoogle Scholar
  47. Patel SKS, Anwar MZ, Kumar A, Otari SV, Pagolu RT, Kim S-Y, Kim I-W, Lee J-K (2018a) Fe O yolk-shel particle-based laccase biosensor for efficient detection of 2,6-dimethoxyphenol. Biochem Eng J 132:1–8. CrossRefGoogle Scholar
  48. Patel SKS, Kim JH, Kalia VC, Lee JK (2018b) Antimicrobial ativity of amino-derivatized cationic polysaccharides. Indian J Microbiol. CrossRefGoogle Scholar
  49. Patel SKS, Lee JK, Kalia VC (2018c) Nanoparticles in biological hydrogen production: an overview. Indian J Microbiol 58:8–18. CrossRefPubMedGoogle Scholar
  50. Patel SKS, Otari SV, Li J, Kim DR, Kim SC, Cho B-K, Kalia VC, Kang YC, Lee J-K (2018d) Synthesis of cross-linked protein-metal hybrid nanoflowers and its application in repeated batch decolorization of synthetic dyes. J Harad Mater 347:442–450. CrossRefGoogle Scholar
  51. Pramual S, Assavanig A, Bergkvist M, Batt CA, Sunintaboon P, Lirdprapamongkol K, Svasti J, Niamsiri N (2016) Development and characterization of bio-derived polyhydroxyalkanoate nanoparticles as a delivery system for hydrophobic photodynamic therapy agents. J Mater Sci Mater Med 27:40. CrossRefPubMedGoogle Scholar
  52. Porwal S, Kumar T, Lal S, Rani A, Kumar S, Cheema S, Purohit HJ, Sharma R, Patel SKS, Kalia VC (2008) Hydrogen and polyhydroxybutyrate producing abilities of microbes from diverse habitats by dark fermentative process. Bioresour Technol 99:5444–5451. CrossRefPubMedGoogle Scholar
  53. Ray S, Kalia VC (2017) Co-metabolism of substrates by Bacillus thuringiensis regulates polyhydroxyalkanoate co-polymer composition. Bioresour Technol 224:743–747. CrossRefPubMedGoogle Scholar
  54. Reusch RN (1989) Poly-β-hydroxybutyrate/calcium polyphosphate complexes in eukaryotic membranes. Proc Soc Exptl Biol Med 191:377–381 doi:NA CrossRefGoogle Scholar
  55. Salman MA, Sahin A, Onur MA, Oge K, Kassab A, Aypar U (2003) Tramadol encapsulated into polyhydroxybutyrate microspheres: in vitro release and epidural analgetic effect in rats. Acta Anaesthesiol Scand 47:1006–1012. CrossRefPubMedGoogle Scholar
  56. Saranya S, Radha KV (2014) Review of nanobiopolymers for controlled drug delivery. Polym Plast Technol Eng 53:1636–1646. CrossRefGoogle Scholar
  57. Schneider-Stock R, Fakhoury IH, Zaki AM, El-Baba CO, Gali-Muhtasib HU (2014) Thymoquinone: fifty years of success in the battle against cancer models. Drug Discov Today 19:18–30. CrossRefPubMedGoogle Scholar
  58. Sendil D, Gürsel I, Wise DL, Hasirci V (1999) Antibiotic release from biodegradable PHBV microparticles. J Control Release 59:207–217. CrossRefPubMedGoogle Scholar
  59. Shah M, Naseer MI, Choi MH, Kim MO, Yoon SC (2010) Amphiphilic PHA–mPEG copolymeric nanocontainers for drug delivery: preparation, characterization and in vitro evaluation. Int J Pharm 400:165–175. CrossRefPubMedGoogle Scholar
  60. Shah M, Ullah N, Choi MH, Kim MO, Yoon SC (2012) Amorphous amphiphilic P (3HV-co-4HB)-b-mPEG block copolymer synthesized from bacterial copolyester via melt transesterification: nanoparticle preparation, cisplatin-loading for cancer therapy and in vitro evaluation. Eur J Pharm Biopharm 80:518–527. CrossRefPubMedGoogle Scholar
  61. Shah M, Ullah N, Choi MH, Yoon SC (2014) Nanoscale poly (4-hydroxybutyrate)-mPEG carriers for anticancer drugs delivery. J NanosciNanotechnol 14:8416–8421. CrossRefGoogle Scholar
  62. Shishatskaya EI, Goreva AV, Voinova ON, Inzhevatkin EV, Khlebopros RG, Volova TG (2008) Evaluation of antitumor activity of rubomycin deposited in absorbable polymeric microparticles. Bull Exp Biol Med 145:358–361. CrossRefPubMedGoogle Scholar
  63. Shishatskaya EI, Nikolaeva ED, Vinogradova ON, Volova TG (2016) Experimental wound dressings of degradable PHA for skin defect repair. J Mater Sci Mater Med 27:165. CrossRefPubMedGoogle Scholar
  64. Shrivastav A, Kim HY, Kim YR (2013) Advances in the applications of polyhydroxyalkanoate nanoparticles for novel drug delivery system. Biomed Res Int 2013:1–12, Article ID 581684. CrossRefGoogle Scholar
  65. Singh M, Patel SKS, Kalia VC (2009) Bacillus subtilis as potential producer for polyhydroxyalkanoates. Microb Cell Factories 8:38. CrossRefGoogle Scholar
  66. Singh M, Kumar P, Patel SKS, Kalia VC (2013) Production of polyhydroxyalkanoate co-polymer by Bacillus thuringiensis. Indian J Microbiol 53:77–83. CrossRefPubMedGoogle Scholar
  67. Singh M, Kumar P, Ray S, Kalia VC (2015) Challenges and opportunities for the customizing polyhydroxyalkanoates. Indian J Microbiol 55:235–249. CrossRefPubMedPubMedCentralGoogle Scholar
  68. Sombatmankhong K, Suwantong O, Waleetorncheepsawat S, Supaphol P (2006) Electrospunfiber mats of poly (3-hydroxybutyrate), poly (3-hydroxybutyrate-co-3-hydroxyvalerate), and their blends. J Polym Sci B Polym Phys 44:2923–2933. CrossRefGoogle Scholar
  69. Tibbitt MW, Dahlman JE, Langer R (2016) Emerging frontiers in drug delivery. J Am Chem Soc 138:704–717. CrossRefPubMedGoogle Scholar
  70. Timbart L, Renard E, Langlois V, Guerin P (2004) Novel biodegradable copolyesters containing blocks of poly (3-hydroxyoctanoate) and poly (ε-caprolactone): synthesis and characterization. Macromol Biosci 4:1014–1020. CrossRefPubMedGoogle Scholar
  71. Türesin F, Gürsel I, Hasirci V (2001) Biodegradable polyhydroxyalkanoate implants for osteomyelitis therapy: in vitro antibiotic release. J Biomater Sci Polym Ed 12:195–207CrossRefGoogle Scholar
  72. Valappil SP, Misra SK, Boccaccini AR, Roy I (2006) Biomedical applications of polyhydroxyalkanoates, an overview of animal testing and in vivo responses. Exp Rev Med Dev 3:853–868. CrossRefGoogle Scholar
  73. Vidal LS, Rojas C, Bouza Padín R, Pérez Rivera M, Haensgen A, González M, Rodríguez-Llamazares S (2016) Synthesis and characterization of polyhydroxybutyrate-co-hydroxyvalerate nanoparticles for encapsulation of quercetin. J Bioact Compat Polym 31:439–452. CrossRefGoogle Scholar
  74. Vilos C, Constandil L, Herrera N, Solar P, Escobar-Fica J, Velásquez LA (2012) Ceftiofur-loaded PHBV microparticles: a potential formulation for a long-acting antibiotic to treat animal infections. Electron J Biotechnol 15:1. CrossRefGoogle Scholar
  75. Vilos C, Morales FA, Solar PA, Herrera NS, Gonzalez-Nilo FD, Aguayo DA, Mendza HL, Comer J, Bravo ML, Gonzalez PA, Kato SP (2013) Paclitaxel-PHBV nanoparticles and their toxicity to endometrial and primary ovarian cancer cells. Biomaterials 34:4098–4108. CrossRefPubMedGoogle Scholar
  76. Xiong YC, Yao YC, Zhan XY, Chen GQ (2010) Application of polyhydroxyalkanoates nanoparticles as intracellular sustained drug-release vectors. J Biomater Sci Polym Ed 21:127–140. CrossRefPubMedGoogle Scholar
  77. Yagmurlu MF, Korkusuz F, Gürsel I, Korkusuz P, Örs Ü, Hasirci V (1999) Sulbactam-cefoperazone polyhydroxybutyrate-co-hydroxyvalerate (PHBV) local antibiotic delivery system: in vivo effectiveness and biocompatibility in the treatment of implant related experimental osteomyelitis. J Biomed Mater Res 46:494–503CrossRefGoogle Scholar
  78. Yao YC, Zhan XY, Zhang J, Zou XH, Wang ZH, Xiong YC, Chen J, Chen GQ (2008) A specific drug targeting system based on polyhydroxyalkanoate granule binding protein PhaP fused with targeted cell ligands. Biomaterials 29:4823–4830. CrossRefPubMedGoogle Scholar
  79. Zawidlak-Węgrzyńska B, Kawalec M, Bosek I, Łuczyk-Juzwa M, Adamus G, Rusin A, Filipczak P, Glowala-Kosinska M, Wolanska K, Krawczyk Z, Kurcok P (2010) Synthesis and antiproliferative properties of ibuprofen–oligo (3-hydroxybutyrate) conjugates. Eur J Med Chem 45:1833–1842. CrossRefPubMedGoogle Scholar
  80. Zhang C, Zhao L, Dong Y, Zhang X, Lin J, Chen Z (2010) Folate-mediated poly (3-hydroxybutyrate-co-3-hydroxyoctanoate) nanoparticles for targeting drug delivery. Eur J Pharm Biopharm 76:10–16. CrossRefPubMedGoogle Scholar
  81. Zhou J, Peng SW, Wang YY, Zheng SB, Wang Y, Chen GQ (2010) The use of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds for tarsal repair in eyelid reconstruction in the rat. Biomaterials 31:7512–7518. CrossRefPubMedGoogle Scholar
  82. Zinn M, Witholt B, Egli T (2001) Occurrence, synthesis and medical application of bacterial polyhydroxyalkanoate. Adv Drug Deliv Rev 53:5–21. CrossRefPubMedGoogle Scholar

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© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Vipin Chandra Kalia
    • 1
  • Subhasree Ray
    • 2
    • 3
  • Sanjay K. S. Patel
    • 1
  • Mamtesh Singh
    • 4
  • Gajendra Pratap Singh
    • 5
  1. 1.Department of Chemical EngineeringKonkuk UniversitySeoulRepublic of Korea
  2. 2.Microbial Biotechnology and GenomicsCSIR – Institute of Genomics and Integrative Biology (IGIB)DelhiIndia
  3. 3.Academy of Scientific & Innovative Research (AcSIR)New DelhiIndia
  4. 4.Department of Zoology, Gargi CollegeUniversity of DelhiDelhiIndia
  5. 5.Mathematical Sciences and Interdisciplinary Research Lab (MathSciIntR-Lab), School of Computational and Integrative SciencesJawaharlal Nehru UniversityNew DelhiIndia

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