In general, the research methodology is divided into several sections. First, the reaction behavior of β-Gal-catalyzed conversion of lactose was studied in order to validate obtained information from the literature. Subsequently, a depth study on the reaction was carried out in the enzymatic hollow fiber membrane reactor. The study mostly emphasizes the effect of transmembrane pressure (TMP) on the β-Gal-catalyzed conversion of lactose. Before that, an appropriate method for the immobilization of β-Gal on the membrane surface was developed. The hydrodynamic study was also carried out in order to choose the most suitable configuration for immobilization and reaction on the hollow fiber membrane. The significance of the effect of TMP on the reaction was analyzed assisted by the coupled kinetics reaction and mass transfer model. The details on the research methodology are described accordingly with the chapter.
- 6.Carreiro-Lewandowski E (2013) Basic principles and practice of clinical chemistry. In: Bishop ML, Fody EP, Schoeff LE (eds) Clinical chemistry: principles, techniques, and correlations, 7th edn. Lippincott Williams and Wilkins, Philadelphia, pp 26–27Google Scholar
- 7.Jönsson A-S (2013) Microfiltration, ultrafiltration and diafiltration. In: Ramaswamy S, Huang HJ, Ramarao BV (eds) Separation and purification technologies in biorefineries, 1st edn. Wiley, Chichester, pp 216–221Google Scholar
- 11.Marangoni AG (2003) Enzyme kinetics: a modern approach. Wiley, New JerseyGoogle Scholar
- 12.Nagy E (2012) Basic equations of the mass transport through a membrane layer. Elsevier, LondonGoogle Scholar