Advertisement

Genetic Engineering for the Improvement of Oil Content and Associated Traits in Jatropha curcas L.

  • Shaik G. Mastan
  • Mangal Singh Rathore
  • Swati Kumari
  • Reddy P. Muppala
  • Nitish KumarEmail author
Chapter

Abstract

Interminably increasing petroleum rates and exhaustion of fossil reserves have ignited a global search for substitutes to renewable fuel sources. Many oil-generating plants, crops and trees have been considered for biofuel; among these Jatropha curcas is regarded as one of the most promising oilseed plants as its seeds contain oil content up to 35%. Because fossil oil consumption is increasing day-by-day, there is an urgent need to enhance the oil content. Transgenic technology is one of the advanced techniques that have been applied to enhance oil content and modify the composition of fatty acids in seed oils. Increasing seed oil content can be done by modifying the enzyme’s level expression in the triacylglycerol biosynthetic pathway. In this chapter, an effort is made to highlight the potential of transgenic technology towards the enhancement of the oil content and in altering the candidate gene expression for biosynthesis of triacylglycerol.

Keywords

Fatty acids Jatropha Kennedy pathway Renewable biodiesel Triacylglycerols 

Notes

Acknowledgments

The manuscript number is PRIS (PRIS- CSIR-CSMCRI - 190/2018).

References

  1. Annarao S, Sidhu OP, Roy R et al (2008) Lipid profiling of developing Jatropha curcas using 1H NMR spectroscopy. Bioresour Technol 99:9032–9035CrossRefGoogle Scholar
  2. Banapurmath NR, Hosmath RS, Girish NM et al (2012) Combustion of Jatropha curcas oil, methyl esters and blends with diesel or ethanol in a CI engine (Ch. 29). In: Carels N, Sujatha M, Bahadur B (eds) Jatropha, challenges for a new energy crop: volume 1: Farming, economics and biofuel. Springer, New York, pp 557–570.  https://doi.org/10.1007/978-1-4614-4806-8_29 CrossRefGoogle Scholar
  3. Brittaine R, Lutaladio N (2010) Jatropha: a smallholder bioenergy crop: the potential for pro-poor development, Integrated Crop Management. Food and Agriculture Organization of the United Nations, RomeGoogle Scholar
  4. Cagliari A, Margis R, Felipe dos SM et al (2011) Biosynthesis of triacylglycerols (TAGs) in plants and algae. Int J Plant Biol 2:e10CrossRefGoogle Scholar
  5. Cahoon EB, Shockey JM, Dietrich CR et al (2007) Engineering oilseeds for sustainable production of industrial and nutritional feedstocks: solving bottlenecks in fatty acid flux. Curr Opin Plant Biol 10:236–244CrossRefGoogle Scholar
  6. Carels N (2009) Jatropha curcas: a review. Adv Bot Res 50:39–86CrossRefGoogle Scholar
  7. Carvalho CR, Clarindo WR, Praca MM et al (2008) Genome size, base composition and karyotype of Jatropha curcas L., an important biofuel plant. Plant Sci 174:613–617CrossRefGoogle Scholar
  8. Ceasar SA, Ignacimuthu S (2011) Applications of biotechnology and biochemical engineering for the improvement of Jatropha and biodiesel: a review. Renew Sust Energ Rev 15:5176–5185CrossRefGoogle Scholar
  9. Chen MS, Wang GJ, Wang RL et al (2011) Analysis of expressed sequence tags from biodiesel plant Jatropha curcas embryos at different developmental stages. Plant Sci 181:696–700CrossRefGoogle Scholar
  10. Costa GGL, Kiara CC, Luís EV et al (2010) Transcriptome analysis of the oil-rich seed of the bioenergy crop Jatropha curcas L. BMC Genomics 11:462CrossRefGoogle Scholar
  11. Dong J, Keller WA, Yan W et al (2004) Gene expression at early stages of Brassica napus seed development as revealed by transcript profiling of seed-abundant cDNAs. Planta 218:483–491CrossRefGoogle Scholar
  12. Durrett TP, Benning C, Ohlrogge J (2008) Plant triacylglycerols as feedstocks for the production of biofuels. Plant J 54:593–607CrossRefGoogle Scholar
  13. Dyer JM, Stymne S, Green AG et al (2008) High value oils from plants. Plant J 54:640–655CrossRefGoogle Scholar
  14. Emil A, Yaakob Z, Kumar MNS et al (2010) Comparative evaluation of physicochemical properties of Jatropha seed oil from Malaysia, Indonesia and Thailand. J Am Oil Chem Soc 87(6):689–695CrossRefGoogle Scholar
  15. Franca MG, Matos AR, Darcy-Lameta A et al (2008) Cloning and characterization of drought stimulated phosphatidic acid phosphatase genes from Vigna unguiculata. Plant Physiol Biochem 46:1093–1100CrossRefGoogle Scholar
  16. Franco MC, Gomes KA, de Carvalho Filho MM et al (2016) Agrobacterium-mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene. Afr J Biotechnol 15:2006–2016CrossRefGoogle Scholar
  17. Gu K, Yi C, Tian D et al (2012) Expression of fatty acid and lipid biosynthetic genes in developing endosperm of Jatropha curcas. Biotechnol Biofuels 5:47CrossRefGoogle Scholar
  18. Gu K, Mao H, Yin Z (2014) Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis δ-endotoxin Cry1Ab/1Ac for resistance to larvae of tortrix moth (Archips micaceanus). Biotechnol Biofuels 7:68CrossRefGoogle Scholar
  19. Gubitz G, Mittelbach M, Trabi M (1999) Exploitation of the tropical oil seed plant Jatropha curcas L. Bioresour Technol 67:73–82CrossRefGoogle Scholar
  20. Jako C, Kumar A, Wei Y et al (2001) Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight. Plant Physiol 126:861–874CrossRefGoogle Scholar
  21. Jiang H, Wu P, Zhang S et al (2012) Global analysis of gene expression profiles in developing physic nut (Jatropha curcas L.) seeds. PLoS One 7:e36522CrossRefGoogle Scholar
  22. Kennedy EP (1961) Biosynthesis of complex lipids. Fed Proc 20:934–940PubMedGoogle Scholar
  23. King AJ, Li Y, Graham IA (2011) Profiling the developing Jatropha curcas L. seed transcriptome by pyrosequencing. Bioenergy Res 4:211–221CrossRefGoogle Scholar
  24. Kley G (2000) Use of genetic engineering in plant breeding-arrival of transgenic crop varieties on the market and public acceptance. Eur J Lipid Sci Technol 6:433–441CrossRefGoogle Scholar
  25. Knothe G (2008) “Designer” biodiesel: optimizing fatty ester composition to improve fuel properties. Energ Fuels 22:1358–1364CrossRefGoogle Scholar
  26. Li MR, Li MQ, Wu GJ (2006) Study on factors influencing Agrobacterium-mediated transformation of Jatropha curcas. Fen Zi Xi Bao Sheng Wu Xue Bao 39:83–89PubMedGoogle Scholar
  27. Li M, Li H, Jiang H et al (2008) Establishment of an Agrobacterium mediated cotyledon disc transformation method for J. curcas. Plant Cell Tissue Organ Cult 92:173–181CrossRefGoogle Scholar
  28. Li R, Yu K, Hildebrand DF (2010) DGAT1, DGAT2 and PDAT expression in seeds and other tissues of epoxy and hydroxy fatty acid accumulating plants. Lipids 45:145–157CrossRefGoogle Scholar
  29. Lindqvist Y, Huang W, Schneider G et al (1996) Crystal structure of delta 9 stearoyl-acyl carrier protein desaturase from castor seed and its relationship to di-iron proteins. EMBO J 15:4081–4092CrossRefGoogle Scholar
  30. Lung SC, Weselake RJ (2006) Diacylglycerol acyltransferase: a key mediator of plant triacylglycerol synthesis. Lipids 41:1073–1088Google Scholar
  31. Mayer KM, Shanklin J (2007) Identification of amino acid residues involved in substrate specificity of plant acyl-ACP thioesterases using a bioinformatics-guided approach. BMC Plant Biol 7:1CrossRefGoogle Scholar
  32. Mazumdar P, Basu A, Paul A et al (2010) Age and orientation of the cotyledonary leaf explants determine the efficiency of de novo plant regeneration and Agrobacterium tumefaciens-mediated transformation in Jatropha curcas L. S Afr J Bot 76:337–344CrossRefGoogle Scholar
  33. Millar AA, Smith MA, Kunst L (2000) All fatty acids are not equal: discrimination in plant membrane lipids. Trends Plant Sci 5:95–101CrossRefGoogle Scholar
  34. Moniruzzaman M, Zahira Y, Rahima K (2016) Biotechnology for Jatropha improvement: a worthy exploration. Renew Sust Energ Rev 54:1262–1277CrossRefGoogle Scholar
  35. Nakamura Y, Tsuchiya M, Ohta H (2007) Plastidic phosphatidic acid phosphatases identified in a distinct subfamily of lipid phosphate phosphatases with prokaryotic origin. J Biol Chem 282:29013–29021CrossRefGoogle Scholar
  36. Nikolau BJ, Ohlrogge JB, Wurtele ES (2003) Plant biotin-containing carboxylases. Arch Biochem Biophys 414:211–222CrossRefGoogle Scholar
  37. Nindita A, Iswari SD, Bambang SP et al (2015) Genetic improvement and biotechnology research of Jatropha curcas Linn. Review: future research opportunity and sustainability challenges in Indonesia. Conference and exhibition Indonesia – new, renewable energy and energy conservation (The 3rd Indo-EBTKE ConEx 2014)Google Scholar
  38. Niu Y, Wu GZ, Ye R et al (2009) Global analysis of gene expression profiles in Brassica napus developing seeds reveals a conserved lipid metabolism regulation with Arabidopsis thaliana. Mol Plant 2:1107–1122CrossRefGoogle Scholar
  39. O’Hara P, Slabas AR, Fawcett T (2002) Fatty acid and lipid biosynthetic genes are expressed at constant molar ratios but different absolute levels during embryogenesis. Plant Physiol 12:9310–9320Google Scholar
  40. Pollard MR, Anderson L, Fan C et al (1991) A specific acyl-ACP thioesterase implicated in medium-chain fatty acid production in immature cotyledons of Umbellularia californica. Arch Biochem Biophys 284:306–312CrossRefGoogle Scholar
  41. Pramanik K (2003) Properties and use of Jatropha curcas oil and diesel fuel blends in compression ignition engine. Renew Energy 28:161–164CrossRefGoogle Scholar
  42. Puente-Rodríguez D (2009) Biotechnologizing Jatropha for local sustainable development. Agric Hum Val 27:351–363CrossRefGoogle Scholar
  43. Purkayastha J, Sugla T, Paul A et al (2010) Efficient in vitro plant regeneration from shoot apices and gene transfer by particle bombardment in Jatropha curcas. Biol Plant 54:13–20CrossRefGoogle Scholar
  44. Purushothaman N, Deepa K, Gnanasekaran G et al (2010) Gene discovery from Jatropha curcas by sequencing of ESTs from normalized and full-length enriched cDNA library from developing seeds. BMC Genomics 11:606CrossRefGoogle Scholar
  45. Qu J, Mao HZ, Chen W et al (2012) Development of marker-free transgenic Jatropha plants with increased levels of seed oleic acid. Biotechnol Biofuels 5:10CrossRefGoogle Scholar
  46. Raorane M, Populechai S, Gatehouse AMR et al (2013) Proteomic perspectives on understanding and improving Jatropha curcas L. In: Bahadur B, Sujatha M, Carels N (eds) Jatropha, challenges for a new energy crop volume 2: Genetic improvement and biotechnology. Springer, New York, pp 375–391Google Scholar
  47. Routaboul JM, Benning C, Bechtold N et al (1999) The TAG1 locus of Arabidopsis encodes for a diacylglycerol acyltransferase. Plant Physiol Biochem 37:831–840CrossRefGoogle Scholar
  48. Ruuska SA, Girke T, Benning C et al (2002) Contrapuntal networks of gene expression during Arabidopsis seed filling. Plant Cell 14:1191–1206CrossRefGoogle Scholar
  49. Salas JJ, Ohlrogge JB (2002) Characterization of substrate specificity of plant FatA and FatB acyl-ACP thioesterases. Arch Biochem Biophys 403:25–34CrossRefGoogle Scholar
  50. Santos-Mendoza M, Dubreucq B, Baud S et al (2008) Deciphering gene regulatory networks that control seed development and maturation in Arabidopsis. The Plant J 54:608–620CrossRefGoogle Scholar
  51. Sato S, Hirakawa H, Isobe S et al (2011) Sequence analysis of the genome of an oil-bearing tree, Jatropha curcas L. DNA Res 18:65–76CrossRefGoogle Scholar
  52. Shockey JM, Gidda SK, Chapital DC et al (2006) Tung tree DGAT1 and DGAT2 have nonredundant functions in triacylglycerol biosynthesis and are localized to different subdomains of the endoplasmic reticulum. Plant Cell 18:2294–2313CrossRefGoogle Scholar
  53. Siloto RM, Truska M, Brownfield D et al (2009) Directed evolution of acyl-CoA:diacylglycerol acyltransferase: development and characterization of Brassica napus DGAT1 mutagenized libraries. Plant Physiol Biochem 47:456–461CrossRefGoogle Scholar
  54. Singh DD, Dipti SD (2010) Biodiesel production through the use of different sources and characterization of oils and their esters as the substitute of diesel: a review. Renew Sust Energ Rev 14:200–216CrossRefGoogle Scholar
  55. Somerville C, Browse J, Jaworski JG et al (2000) Lipids. In: Buchanan BB, Gruissem W, Jones RL (eds) Biochemistry and molecular biology of plants. American Society of Plant Physiologists, Rockville, pp 456–527Google Scholar
  56. Sood A, Singh R, Chauhan S (2015) Regulation of FA and TAG biosynthesis pathway genes in endosperms and embryos of high and low oil content genotypes of Jatropha curcas L. Plant Physiol Biochem 94:253–267CrossRefGoogle Scholar
  57. Stymne SS (1987) Triacylglycerol biosynthesis. In: The biochemistry of plants: a comprehensive treatise. Academic, Orlando, pp 175–214Google Scholar
  58. Tai H, Jaworski JG (1993) 3-Ketoacyl-acyl carrier protein synthase III from spinach (Spinacia oleracea) is not similar to other condensing enzymes of fatty acid synthase. Plant Physiol 103:1361–1367CrossRefGoogle Scholar
  59. Tiwari AK, Kumar A, Raheman H (2007) Biodiesel production from Jatropha oil (Jatropha curcas) with high free fatty acids: an optimized process. Biomass Bioenergy 31:569–575CrossRefGoogle Scholar
  60. Tong L, Shu-Ming P, Wu-Yuan D et al (2006) Characterization of a new stearoyl-acyl, carrier protein desaturase gene from Jatropha curcas. Biotechnol Lett 28:657–662CrossRefGoogle Scholar
  61. Troncoso-Ponce MA, Kilaru A, Cao X et al (2011) Comparative deep transcriptional profiling of four developing oil seeds. Plant J 68:1014–1027CrossRefGoogle Scholar
  62. Turkish AR, Henneberry AL, Cromley D et al (2005) Identification of two novel human acyl-CoA wax alcohol acyltransferases: members of the diacylglycerol acyltransferase 2 (DGAT2) gene superfamily. J Biol Chem 280:14755–14764CrossRefGoogle Scholar
  63. Voelker T, Kinney AJ (2001) Variations in the biosynthesis of seed-storage lipids. Annu Rev Plant Physiol Plant Mol Biol 52:335–361CrossRefGoogle Scholar
  64. Weselake RJ, Taylor DC, Rahman MH et al (2009) Increasing the flow of carbon into seed oil. Biotechnol Adv 27:866–878CrossRefGoogle Scholar
  65. Xu J, Francis T, Mietkiewska E et al (2008) Cloning and characterization of an acyl-CoA-dependent diacylglycerol acyltransferase 1 (DGAT1) gene from Tropaeolum majus, and a study of the functional motifs of the DGAT protein using site-directed mutagenesis to modify enzyme activity and oil content. Plant Biotechnol J 6:799–818CrossRefGoogle Scholar
  66. Xu R, Wang R, Liu A (2011) Expression profiles of genes involved in fatty acid and triacylglycerol synthesis in developing seeds of Jatropha (Jatropha curcas L.). Biomass Bioenergy 35:1683–1692CrossRefGoogle Scholar
  67. Yang MF, Liu YJ, Liu Y et al (2009) Proteomic analysis of oil mobilization in seed germination and post germination development of Jatropha curcas. J Proteome Res 8(3):1441–1451CrossRefGoogle Scholar
  68. Ye J, Hong Y, Qu J et al (2013) Improvement of J. curcas oil by genetic transformation. In: Bahadur B, Sujatha M, Carels N (eds) Jatropha, challenges for a new energy crop volume 2: Genetic improvement and biotechnology. Springer, New York, pp 547–562Google Scholar
  69. Zhang Y, Wang Y, Jiang L et al (2007) Aquaporin JcPIP2 is involved in drought responses in Jatropha curcas. Acta Biochim Biophys Sin 39(10):787–794CrossRefGoogle Scholar
  70. Zhang F, Niu B, Wang Y et al (2008) A novel betaine aldehyde dehydrogenase gene from Jatropha curcas, encoding an enzyme implicated in adaptation to environmental stress. Plant Sci 174:510–518CrossRefGoogle Scholar
  71. Zou J, Wei Y, Jako C et al (1999) The Arabidopsis thaliana TAG1 mutant has a mutation in a diacylglycerol acyltransferase gene. Plant J 19:645–653CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Shaik G. Mastan
    • 1
  • Mangal Singh Rathore
    • 2
  • Swati Kumari
    • 3
  • Reddy P. Muppala
    • 4
  • Nitish Kumar
    • 5
    Email author
  1. 1.Aditya Degree and PG CollegeKakinadaIndia
  2. 2.Marine Biotechnology and Ecology DivisionCentral Salt and Marine Chemicals Research InstituteBhavnagarIndia
  3. 3.Department of Life Science, School of Earth, Biological and Environmental SciencesCentral University of South BiharGayaIndia
  4. 4.Center for Desert AgricultureKing Abdullah University of Science and TechnologyThuwalSaudi Arabia
  5. 5.Department of Biotechnology, School of Earth, Biological and Environmental SciencesCentral University of South BiharGayaIndia

Personalised recommendations