Isolation, Identification and Characterization of Fungi from Vinoba Bhave University Campus, Hazaribag District

  • Kumar Anand
  • Thirupathi Karuppanapandian
  • Pritam Bala SinhaEmail author
Conference paper


Fungi play a very significant role in the soil complex, and equally, it is very important for the soil ecosystem. In the present study, seven different soil samples were collected from the Vinoba Bhave University Campus of Hazaribag district. Altogether, 58 fungal strains were isolated and among them 20 strains were characterized further using various isolation and identification methods. Soil samples were also characterized for their physico-chemical properties. The isolates exhibited a range of colony colours such as whitish, yellowish white, greenish, dark greenish, blackish, pinkish and reddish also. Prominent among these isolates are the Penicillium notatum, Penicillium chrysogenum, Cladosporium sphaerospermum, Fusarium spp., Aspergillus spp. and others. Further work is also being done on biochemical characterization of these isolates; especially, their amylase, cellulase and phosphatase activities are being investigated.


Soil microbiota Saprophytic Penicillium notatum Penicillium chrysogenum Cladosporium sphaerospermum 



The author is thankful to Dr. M. A. Mallick, Director, Biotechnology, and Dr. P. K. Mishra, Head of the Department, Vinoba Bhave University, Hazaribag, Jharkhand, for providing necessary support and facilities to conduct this work in the Microbiology Laboratory of the Department.

Conflict of Interest

There was no conflict of interest in the present study.


  1. 1.
    Andy Purvis & Andy Hector (2000). Getting the measure of biodiversity, Nature 405, 212–219. Scholar
  2. 2.
    Anand Kumar & Pandey Gaurav Kumar (2014). Estimation of microbial diversity in some selected wetlands: a case study of North Bihar. International Journal of Advanced Life Sciences: Vol. 7 (02), pp 217–222.
  3. 3.
    Anand Kumar, Kumari Baby & Mallick M.A. (2016). Phosphate Solubilizing Microbes: An effective and alternative approach as biofertilizers. International Journal of Pharmacy and Pharmaceutical Sciences. Vol. 8(02), pp 37–40.
  4. 4.
    Aghamirian M.R. & Ghiasian S.A. (2012). The prevalence of fungi in soil of Qazvin, Iran, Jundishapur, J. Mircobial:6 (1):76–9. Scholar
  5. 5.
    Waksman S.A. (1922). A method of counting the number of fungi in soil. J. Bact.7: 339–341.
  6. 6.
    Waksman S.A. (1922). A method of counting the number of fungi in soil. J. Bact. 7: 339–341.
  7. 7.
    Booth, C. (1971). The genus fusarium commonwealth mycological institute. Kew. Surrey237.
  8. 8.
    Cheesbrough M. (2000). District Laboratory Practice in Tropical Countries. Part 2. Cambridge University Press, Cambridge, U.K. ISBN: 9780521676311Google Scholar
  9. 9.
    Christensen M. (1989). A view of fungal ecology. Mycologia, 81:1–19. Scholar
  10. 10.
    Finlay R, D. (2007). The fungi in soil. In: van Elsas JD, Jansson JK, Trevors JT (Eds.) Modern Soil Microbiology, CRC Press, New York, pp: 107–146. Online LibraryGoogle Scholar
  11. 11.
    Grifffin D.M. (1972). Ecology of Soil Fungi. Chapman & Hall, London pp 193. Online LibraryGoogle Scholar
  12. 12.
    Klich M.A. (2002). Biogeography of Aspergillus species in soil and litter. Mycologia. 94 (1):21–27. Scholar
  13. 13.
    Alexender M. (1977). Introduction to Soil Microbiology. 2nd Edition. John Wiley & Sons, New York, pp 423–437. Online LibraryGoogle Scholar
  14. 14.
    Harold J.B. (2002). Microbiological applications. Laboratory Manuals in General Microbiology, 8th Edition, McGraw-Hill Higher Education. Online LibraryGoogle Scholar
  15. 15.
    Dubey R.C. & Maheshwari, D. K. (2007). A manual of practical microbiology. S. Chand and Company limited, Ramnagar New Delhi 1–397.Google Scholar
  16. 16.
    James C.C. & Natalie S. (2001). Microbiology –A Laboratory Manual. (Ed). pp 211–223.Google Scholar
  17. 17.
    Warcup J.H. (1951). On the origin of colonies of fungi developing on soil. Vol. 62:4291.Google Scholar
  18. 18.
    Molin & Molin (1997). Defining Soil Quality for a Sustainable Environment. American Society for Agronomy, SSSA Special Publication No. 35, Madison, WI.Google Scholar
  19. 19.
    Dix N.J. & Webster, J. (1995). Fungal Ecology. Chapman Hall, Ch7. Online LibraryGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Kumar Anand
    • 1
  • Thirupathi Karuppanapandian
    • 2
  • Pritam Bala Sinha
    • 3
    Email author
  1. 1.University Department of BiotechnologyVinoba Bhave UniversityHazaribagIndia
  2. 2.Department of Experimental Biology, Faculty of ScienceMasaryk UniversityBrnoCzech Republic
  3. 3.Amity Institute of Biotechnology, Amity University JharkhandRanchiIndia

Personalised recommendations