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Bioremediation of Lead Compound Using Acinetobacter sp. 158; Optimization of Media and Cell Growth Dynamics

  • Moumita BoseEmail author
  • S. Datta
  • Pinaki Bhattacharya
Conference paper

Abstract

The present investigation deals with an in-depth study on optimization of lead removal capacity of lead-resistant bacterium Acinetobacter sp. 158 as a response variable with limiting carbon source, pH, and rotational speed as non-interacting-type independent variables. The statistical optimization technique response surface methodology has been used for this purpose. The optimum parameters of the independent variables have been identified through 3D plots. A set of deterministic model equations have been developed through rigorous modeling and simulation to predict the cell growth dynamics of the lead-resistant bacterium Acinetobacter sp. 158. A set of programmed experiments have been conducted using the optimized value of the independent variables obtained through RSM to validate the proposed model equations.

Nomenclatures

CG

Concentration of substrate glucose (kg/m3)

CB

Concentration of biomass (kg/m3)

k

Maximum specific cell growth rate (hour−1)

KS

Saturation constant for lead (kg/m3)

t

Time of incubation (hours)

\(\varvec{Y}_{{{\raise0.7ex\hbox{$\varvec{B}$} \!\mathord{\left/ {\vphantom {\varvec{B} \varvec{G}}}\right.\kern-0pt} \!\lower0.7ex\hbox{$\varvec{G}$}}}}\)

Yield factor

Notes

Acknowledgements

One of the authors (M.B.) gratefully acknowledges the University Grants Commissions, Govt. of India for providing financial support for this work.

References

  1. 1.
    Wang X, Nie Z, He L, Wang Q, Sheng X (2017) Isolation of As—tolerant bacteria and their potentials of reducing As and Cd accumulation of edible tissues of vegetables in metal (loid)-contaminated soils. Sci Total Env 579:179–189CrossRefGoogle Scholar
  2. 2.
    Mahbub MR, Kannan K, Naidu R, Mallavarapu M (2016) Mercury resistance and volatilization by Pseudoxanthomonas sp. SE1 isolated from soil. Environ Technol Innov 6:94–104CrossRefGoogle Scholar
  3. 3.
    Mala JGS, Sujatha D, Rose C (2015) Inducible chromate reductase exhibiting extracellular activity in Bacillus methylotrophicus for chromium bioremediation. Microbiol Res 170:235–241CrossRefGoogle Scholar
  4. 4.
    Davolos D, Pietrangeli B (2013) A molecular study on bacterial resistance to arsenic-toxicity in surface and underground waters of Latium (Italy). Ecotoxicol. Environ Saf 96:1–9CrossRefGoogle Scholar
  5. 5.
    Bose M, Datta S, Bhattacharya P (2017) Studies on isolation, characterization and cell growth dynamics of a lead resistant bacterium Acenetobacter 158. Environ Technol Innov 8:103–112CrossRefGoogle Scholar
  6. 6.
    Ghoshal S, Bhattacharya P, Chowdhury R (2011) De-mercurization of wastewater by Bacillus cereus (JUBT1): Growth kinetics, biofilm reactor study and field emission scanning electron microscopic analysis. J Hazard Mater 194:355–361CrossRefGoogle Scholar
  7. 7.
    Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. J Anal Chem 31:426–428CrossRefGoogle Scholar
  8. 8.
    Myers Raymond H, Montgomery DC (2002) Response surface methodology: process and product optimization using designed experiment. Wiley-Interscience Publication, New York, NYGoogle Scholar
  9. 9.
    Box G, Hunter JS (1957) Multi-factor experimental designs for exploring response surfaces. Ann Math Stat 28:195–241CrossRefGoogle Scholar
  10. 10.
    Ferda G, Zümriye A (2008) Use of response surface methodology (RSM) in the evaluation of growth and copper(II) bioaccumulation properties of Candida utilis in molasses medium. J Haz Mat 154:731–738CrossRefGoogle Scholar
  11. 11.
    Levenspiel O (2013) Chemical Reactor Omnibook-soft cover. Lulu. com.Google Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  1. 1.Chemical Engineering DepartmentJadavpur UniversityKolkataIndia
  2. 2.Department of Chemical EngineeringHeritage Institute of TechnologyKolkataIndia

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