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Agrobacterium-Mediated Transformation for Insect-Resistant Plants

  • B. Sunil Kumar
  • C. Immanuel Selvaraj
Chapter

Abstract

Bacillus thuringiensis (Bt) is considered as gram-positive, aerobic, spore-forming, naturally occurring facultative soilborne bacterial pathogen and has been used for natural insect control. It produces a parasporal, persistent insecticidal protein crystals (ICPs). These ICPs are toxic in nature for a class of lepidopterans, dipterans, and coleopterans. That toxic protein differs, depending on the subspecies of Bt producing it. The most prevalent ICPs are the Cry (crystal) protein, and the other is Cty (cytolytic) protein produced by some Bacillus thuringiensis stains. The Cry proteins, in general were cleaved by proteolytic enzymes on intake to produce active toxins which results in osmotic imbalance, lysis of epithelial cells, and finally death due to starvation, whereas Cty proteins release vegetative insecticidal proteins (VIPs) which lead to membrane disruptions, midgut lysis, and paralysis in lepidopterans pests. The use of ICPs as a pesticide or insecticide over chemicals is more beneficial as there is less amount of environmental pollution and harmful chemical residues leaching into the soil and water bodies. It is also target specific and acts on specific class of pests and at the same time harmless to birds, fish, and mammals whose acidic gut conditions negate the bacteria’s effect.

Keywords

Bacillus thuringiensis (BtInsecticidal Protein crystals (ICPs) Major pests Cultivated crops 

Notes

Acknowledgment

The authors would like to thank authorities of Annamalai University and VIT School of Agricultural Innovations and Advanced Learning, Vellore, for their encouragement and support.

References

  1. Aldemita, R. R., & Hodges, T. K. (1996). Agrobacterium tumefaciens-mediated transformation of Japonica and Indica rice varieties. Planta, 199, 612–617.CrossRefGoogle Scholar
  2. Aljanabi, S. M., Forget, L., & Dooklun, A. (1999). An improved and rapid protocol for the isolation of polysaccharide and polyphenol free sugarcane DNA. Plant Molecular Biology Reporter, 17, 1–8.CrossRefGoogle Scholar
  3. Cho, S. K., Chung, Y. S., Park, S. J., Shin, J. S., Kwon, H. J., & Kang, K. H. (1998). Efficient transformation of Korean rice cultivars (Oryza sativa L.) mediated by agrobacterium tumefaciens. Journal of Plant Biology, 41, 262–268.CrossRefGoogle Scholar
  4. Chu, C. C., Wangand, C. C., & Sun, C. S. (1975). Establishment of an efficient medium for another culture of rice through comparative experiments on the nitrogen sources. Scientia Sinica, 18, 659–668.Google Scholar
  5. Dong, J., Teng, W., Buchholz, W. G., & Hall, T. C. (1996). Agrobacterium-mediated transformation of Javanica rice. Molecular Breeding, 2, 267–276.CrossRefGoogle Scholar
  6. Doyle, J. J., & Doyle, J. L. (1990). A rapid total DNA preparation procedure for fresh plant tissue. Focus, 12, 13–15.Google Scholar
  7. Hiei, Y., Ohta, S., Komari, T., & Kumashiro, T. (1994). Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. The Plant Journal, 6, 271–282.CrossRefGoogle Scholar
  8. Hiei, Y., Komari, T., & Kubo, T. (1997). Transformation of rice mediated by agrobacterium tumefaciens. Plant Molecular Biology, 35, 205–218.CrossRefGoogle Scholar
  9. Hilder, V. A., & Boulter, D. (1999). Genetic engineering of crop plants for insect resistance – A critical review. Crop Protection, 18, 177–191.CrossRefGoogle Scholar
  10. Islam, N., Laksana, C., & Chanprame, S. (2016). Agro bacterium – Mediated transformation and expression of Bt gene in transgenic sugarcane. Journal of The International Society for Southeast Asian Agricultural Sciences, 22(1), 84–95.Google Scholar
  11. Laksana, C., & Chanprame, S. (2015). A simple and rapid method for RNA extraction from young and mature leaves of oil palm (Elaeis guineensis Jacq.). Journal of the International Society for Southeast Asian Agricultural Sciences, 21(1), 96–106.Google Scholar
  12. Lucena, W. A., Pelegrini, P. B., Martins-de-Sa, D., Fonseca, F. C. A., Gomes, J. E., de Macedo, L. L. P., da Silva, M. C. M., Oliveira, R. S., & Grossi-de-Sa, M. F. (2014). Molecular approaches to improve the insecticidal activity of bacillus thuringiensis cry toxins. Toxins, 6(8), 2393–2423.  https://doi.org/10.3390/toxins6082393.CrossRefPubMedPubMedCentralGoogle Scholar
  13. Matzke, A. J. M., & Chilton, M. D. (1981). Sitespecific insertion of genes into the T-DNA of the Agrobacterium tumor inducing plasmid: An approach to genetic engineering of higher plant cells. Journal of Molecular and Applied Genetics, 1, 39–49.PubMedGoogle Scholar
  14. Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473–497.CrossRefGoogle Scholar
  15. Nishimura, A., Ashikari, M., Lin, S., Takashi, T., Angeles, E. R., Yamamoto, T., & Matsuoka, M. (2005). Isolation of a rice regeneration QTL gene and its application to new transformation system. In Proceedings of National Academy of Sciences of the USA (Vol. 102, pp. 11940–11944).Google Scholar
  16. Nishimura, A., Aichi, I., & Matsuoka, M. (2006). A protocol for agrobacterium-mediated transformation in rice. Nature Protocols, 1(6), 2796–2802.  https://doi.org/10.1038/nprot.2006.469.CrossRefPubMedGoogle Scholar
  17. Prasad, B. D., Kumar, P., Sahni, S., Kumar, V., Kumari, S., Kumar, P., & Pal, A. K. (2016). An improved protocol for agrobacterium-mediated genetic transformation and regeneration of Indica rice (Oryza sativa L. var. Rajendra Kasturi). Journal of Cell Tissue Research, 16(2), 5597–5606.Google Scholar
  18. Rachmawati, D., Hosaka, T., Inoue, E., & Anzai, H. (2004). Agrobacterium-mediated transformation of Javanica rice cv. Rojolele. Bioscience Biotechnology and Biochemistry, 68, 1193–1200.CrossRefGoogle Scholar
  19. Rashid, H., Yokoi, S., Toriyama, K., & Hinata, K. (1996). Transgenic plant production mediated by agrobacterium in Indica rice. Plant Cell Reports, 15, 727–730.CrossRefGoogle Scholar
  20. Sambrook, J., & Russell, D. (2001). Molecular cloning: A laboratory manual (3rd ed.). Cold Spring Harbor: Cold Spring Harbor Laboratory Press.Google Scholar
  21. Supartana, P., Shimizu, T., Shioiri, H., Nogawa, M., Nozue, M., & Kojima, M. (2005). Development of simple and efficient in planta transformation method for rice (Oryza sativa L.) using agrobacterium tumefaciens. Journal of Bioscience and Bioengineering, 100, 391–397.CrossRefGoogle Scholar
  22. Toki, S. (1997). Rapid and efficient agrobacterium-mediated transformation in rice. Plant Molecular Biology Reports, 15, 16–21.CrossRefGoogle Scholar
  23. Yara, A., Otani, M., Kusumi, K., Matsuda, O., Shimada, T., & Iba, K. (2001). Production of transgenic japonica rice (Oryza sativa) cultivar, Taichung 65, by the agrobacterium-mediated method. Plant Biotechnology, 18, 305–310.CrossRefGoogle Scholar
  24. Zhang, J., Xu, R., Elliott, M. C., & Chen, D. F. (1997). Agrobacterium-mediated transformation of elite Indica and Japonica rice cultivars. Molecular Biotechnology, 8, 223–231.CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • B. Sunil Kumar
    • 1
  • C. Immanuel Selvaraj
    • 2
  1. 1.Department of Genetics and Plant Breeding, Faculty of AgricultureAnnamalai UniversityChidambaramIndia
  2. 2.VIT School of Agricultural Innovations and Advanced LearningVellore Institute of TechnologyVelloreIndia

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