Germplasm Screening and Evaluation Techniques Against Insect Pests



Standardized screening and evaluation procedures are required to precisely locate resistant/tolerant genotypes in a germplasm. Such procedures ensure comparison of data from different geographical location screening, and evaluation procedures are done in the field, greenhouse, and laboratory depending on the requirements. Depending on the goals set or desired, the precision and comprehensiveness of screening procedures vary. Germplasm screening procedures for certain key pests on few major crops are described in this chapter. These are quantitative in nature, suitable for statistical analyses.


Germplasm screening Evaluation parameters Standardization Statistical analysis 



The authors would like to thank the authorities of Annamalai University, Tamil Nadu, and International Rice Research Institute, Los Baños, Laguna, Philippines, for their encouragement and support. Authors also acknowledge various internet sources for the photographs.


  1. Anonymous. (1986). Proceedings of VII annual Rabi-summer groundnut workshop (pp. 4–5). Rahuri: Mahatma Phule Agricultural University.Google Scholar
  2. Atwal, A. S., & Dhaliwal, G. S. (2015). Agricultural pests of South Asia and their management. New Delhi: Kalyani Publishers.Google Scholar
  3. Balaji, K., & Selvanarayanan, V. (2009). Evaluation of resistance in sesame germplasm against shoot Webber and capsule borer, Antigastra catalaunalis. Indian Journal of Plant Protection, 37, 35–38.Google Scholar
  4. Dangle, J. L., & Jones, J. D. (2001). Plant pathogens and integrated defense responses to infection. Nature, (6839), 826–833.Google Scholar
  5. David, H., Easwaramoorthy, S., & Jayanthi, R. (1986). Sugarcane entomology in India (p. 564). Coimbatore: Sugarcane Breeding Institute.Google Scholar
  6. Heinrichs, E. A., Medrano, F. G., & Rapusas, H. R. (1985). Genetic evaluation for insect resistance in rice (p. 356). Manila: International Rice Research Institute.Google Scholar
  7. IRRI. (1996). Standard evaluation system for rice (4th ed.). IRRI. PO Box 933 Manila Philippines, p. 52.Google Scholar
  8. Kavitha, K., & Reddy, K. D. (2012). Screening techniques for different insect pests in crop plants regional agricultural Research Station (ANGRAU), Palem, Mahabubnagar, Andhra Pradesh. Indian International Journal of Bio-resource and Stress Management, 3(2), 188–195.Google Scholar
  9. Lateef, S. S., & Sachan, J. N. (1990). Host plant resistance to Helicoverpa armigera (Hubner) in different agro-ecological context. In Chickpea in nineties. Proceedings of the second international workshop on chickpea (pp. 181–189). Patancheru: ICRISAT Center.Google Scholar
  10. Panda, N., & Khush, G. S. (1995). Host plant resistance to insects (p. 315). Wallingford: CAB International.Google Scholar
  11. Prahalada, G. D., Shivakumar, N., Lohithaswa, H. C., Sidde Gowda, D. K., Ramkumar, G., Kim, S.-R., Ramachandra, C., Hittalmani, S., Mohapatra, T., & Jena, K. K. (2017). Identification and fine mapping of a new gene, BPH31 conferring resistance to brown planthopper biotype 4 of India to improve rice, Oryza sativa L. Rice, 10, 41. Scholar
  12. Prakasa Rao, P. S. (1975). Some methods of increasing field infestations of rice gall midge. Rice Entomological Newsletters, 2, 16–17.Google Scholar
  13. Rajendran, B., Haneefa, A. M., Umapathy, G., & Venkatakrishnan, J. (1998). Cultural measures to curb shoot borer menace in sugarcane. Insect Environment, 4(2), 48–49.Google Scholar
  14. Rao, N. (1973). An index for Jassid resistance in cotton. Madras Agricultural Journal, 60(4), 264–266.Google Scholar
  15. Selvanarayanan, V. (2000). Host plant resistance in tomato against fruit borer, Heliothis armigera (Hub.). Ph.D. thesis submitted to the Annamalai University, Annamalainagar, India.Google Scholar
  16. Sharma, H. C., Taneja, S. L., Leuschner, K., & Nwanze, K. F. (1992). Techniques to screen sorghums for resistance to insect pests. Information Bulletin No. 32. International Crops Research Institute for the Semi-Arid Tropics, Patancheru, Andhra Pradesh, India, pp. 4–8.Google Scholar
  17. Smith, C. M., Khan, Z. R., & Pathak, M. D. (1994). Techniques for evaluating insect resistance in crop plants. Boca Raton: Lewis Publishers.Google Scholar
  18. Sridhar, R. P., & Gopalan, M. (2002). Studies on screening and mechanism of resistance against the shoot Webber. Antigastra catalaunalis (Duponchel). Entomon, 27(4), 365–373.Google Scholar
  19. Vijai Anandh, G. (2003). Host plant resistance in sesame against shoot webber and capsule borer, Antigastra catalaunalis Dup. and phyllody disease. M. Sc. (Ag.) Dissertation submitted to the Annamalai University, Annamalai Nagar, India.Google Scholar
  20. Vreden, G. V., & Arifin, K. (1977). Screening rice varieties for resistance to the rice gall midge, Orseolia oryzae (Wood-Mason) Contrib. Cent. Res. Inst. Agric. Bogor, Indonesia, p. 14.Google Scholar

Further Reading

  1. Khan, Z., & Saxena, R. C. (1990). Purification of biotype I population of brown planthopper Nilaparvata lugens (Homoptera: Delphacidae). International Journal of Tropical Insect Science, 11(01), 55–62.CrossRefGoogle Scholar
  2. Saxena, R. C., & Khan, Z. R. (1991). Electronic recording of feeding behavior of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) on resistant and susceptible rice cultivars. Annals of the Entomological Society of America, 84(3), 316–318.CrossRefGoogle Scholar
  3. Tomaz, A. C., Coutinho, A. E., Soares, B. O., Peternelli, L. A., Pereira, E. J. G., & Barbosa, M. H. P. (2017). Assessing resistance of sugarcane varieties to sugarcane borer Diatraea saccharalis fab. (Lepidoptera: Crambidae). Bulletin of Entomological Research, 108(4), 1–9.Google Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  1. 1.Department of EntomologyAnnamalai UniversityChidambaramIndia
  2. 2.Strategic Innovation PlatformInternational Rice Research InstituteLos BañosPhilippines

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