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Methylene Blue-Mediated Photoinactivation of Staphylococcus aureus Assisted by Gold Nanoshells

  • Sajid Farooq
  • Thâmara Tallita da Silva Correia
  • Tania Matheus Yoshimura
  • Saulo de Toledo Pereira
  • Martha Simões Ribeiro
  • Renato E. de AraujoEmail author
Conference paper
Part of the IFMBE Proceedings book series (IFMBE, volume 70/2)

Abstract

Gold nanoshells (silica core/gold shell) have fascinating optical extinction spectrum within visible to near infrared range. In this work, we investigate the optical properties of Au nanoshells and evaluate the feasibility of their use on photodynamic therapy. Three dimensional finite element simulation and experimental analyses were explored on the assessment of the localized surface plasmon resonance spectrum and spatial distribution of the electromagnetic field enhancement near metallic nanoshells. In addition, the interaction of Au nanoshells with methylene blue (MB) photosensitizer was appraised, and 3.2—fold metal enhanced single oxygen generation was observed. Lastly, we investigate the effects of MB-mediated photoinactivation on Staphylococcus aureus assisted by gold nanoshells. The use of nanoshells on the photoinactivation procedure reduced 3× the required illumination time for total eradication of bacterial cells. Our results indicate that Au nanoshells are promising candidates to enhance the photodynamic effect on bacterial cells.

Keywords

Bacterial cells Localized surface plasmon resonance Gold nanoshells Photodynamic therapy 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Sajid Farooq
    • 1
  • Thâmara Tallita da Silva Correia
    • 1
  • Tania Matheus Yoshimura
    • 2
  • Saulo de Toledo Pereira
    • 2
  • Martha Simões Ribeiro
    • 2
  • Renato E. de Araujo
    • 1
    Email author
  1. 1.Federal University of PernambucoRecifeBrazil
  2. 2.Center for Lasers and Applications, IPEN, CNEN/SPRecifeBrazil

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