Abstract
Cell suspension cultures provide a convenient way in understanding the dynamics growth of plant cells. This knowledge is crucial in order to manipulate all parameters related to their growth so that the potential of plants can be fully utilised. Growth can be defined as the increase of cell numbers, size or mass. In a growth cycle, cells will be experiencing four phases which are lag, logarithmic (exponential), stationary and death phase. The information and knowledge gained from growth kinetics parameters provide the basic platform for modelling and predicting the characteristics of cell culture. This chapter describes the procedures for fresh weight, dry weight, TCN, medium conductivity (pH) and residual sugar analysis.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Albiol J, Robusté J, Casas C, Poch M (1993) Biomass estimation in plant cell cultures using an extended Kalman filter. Biotechnol Prog 9(2):174–178
Amino S-i, Tazawa M (1988) Uptake and utilization of sugars in cultured rice cells. Plant Cell Physiol 29(3):483–487
Evans D, Coleman J, Kearns A (2003) Plant cell culture: the basics. Taylor & Francis, London
Fowler MW (1982) Substrate utilisation by plant-cell cultures. J Chem Technol Biotechnol 32(1):338–346
Kato A, Nagai S (1979) Energetics of tobacco cells, Nicotiana tabacum L., growing on sucrose medium. Appl Microbiol Biotechnol 7(3):219–225
Pareilleux A, Chaubet N (1980) Growth kinetics of apple plant cell cultures. Biotechnol Lett 2(6):291–296
Puad NIM (2011) Modelling the metabolism of plant cell culture (PhD), University of Manchester
Son SH, Choi SM, Lee YH, Choi KB, Yun SR, Kim JK et al (2000) Large-scale growth and taxane production in cell cultures of Taxus cuspidata (Japanese yew) using a novel bioreactor. Plant Cell Rep 19(6):628–633
Sturm A (1999) Invertases. Primary structures, functions, and roles in plant development and sucrose partitioning. Plant Physiol 121(1):1–8. https://doi.org/10.1104/pp.121.1.1
Wylegalla C, Meyer R, Wagner KG (1985) Nucleotide pools in suspension-cultured cells of Datura innoxia. Planta 166(4):446–451
Acknowledgement
The authors would like to acknowledge the Ministry of Higher Education in Malaysia for funding this project under the Fundamental Research Grant Scheme (FRGS 14-145-0386).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Nature Singapore Pte Ltd.
About this chapter
Cite this chapter
Mohamad Puad, N.I., Abdullah, T.A. (2018). Monitoring the Growth of Plant Cells in Suspension Culture. In: Amid, A., Sulaiman, S., Jimat, D., Azmin, N. (eds) Multifaceted Protocol in Biotechnology. Springer, Singapore. https://doi.org/10.1007/978-981-13-2257-0_17
Download citation
DOI: https://doi.org/10.1007/978-981-13-2257-0_17
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-13-2256-3
Online ISBN: 978-981-13-2257-0
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)