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Current Approaches and Key Applications of Plant Metabolic Engineering

  • Bharti
  • Reetu
  • Vinay KumarEmail author
Chapter

Abstract

The diversion of carbon flux toward biosynthesis of targeted products could be achieved by manipulation of targeted biosynthesis pathway in plants. This whole process consists of many steps in stepwise manners starting with the identification and isolation of targeted metabolites, elucidation of complete biosynthetic pathway for identification of point of intervention, discovery of corresponding potential metabolic genes, and overexpression of the selected genes in heterologous system and collectively production of the metabolites. The various biochemical processes including transcriptome, translatome, proteome, and reactome are being used to assist metabolic engineering by providing new insights into novel pathways or bottlenecks of existing pathways. Apart from all these, in-depth understanding of metabolic fluxes and feedback regulations is also mandatory for plant metabolic engineering. All these different current approaches are collectively considered for investigating the plant metabolic engineering to understand, reconstruct, analyze, and annotate the targeted pathways. The key applications of plant metabolic engineering have been compiled with a few important applications including improvement of nitrogen utilization in plant, development of highly nutritive food, and generation of biofuel production. In conclusion, the plant metabolic engineering could provide comprehensive evaluation of manipulation of biosynthetic pathways for numerous applications. This compiled information could act as a resource for crop breeding and biotechnology purposes.

Keywords

Plant Metabolic engineering Proteins Metabolites 

Abbreviations

RBS

Ribosomal binding site

NNAAs

Nonnatural amino acids

ORF

Open reading frame

TAG

Triacylglycerol

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Department of Plant Sciences, School for Basic and Applied SciencesCentral University of Punjab (CUPB)BathindaIndia

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