Phytoremediation of Light Non-Aqueous Phase Liquids

  • Sunday Oniosun
  • Michael HarbottleEmail author
  • Snehasis Tripathy
  • Peter Cleall
Conference paper
Part of the Environmental Science and Engineering book series (ESE)


Non-aqueous phase liquids (NAPLs) are persistent sources of contamination in the ground, providing a long-term supply of dissolved phase contamination and taking significant periods to dissipate naturally. Light NAPLs (LNAPLs) take the form of a separate phase within the ground, often as individual ganglia in pore spaces within the capillary zone such that the contaminated region is diffuse and comprised of many unconnected small contaminant sources. Consequently, remedial action is challenging and success may be limited to ex-situ remediation techniques. The ability of plants to phytoremediate dissolved-phase contamination is well known, but the impact of LNAPLs on plant growth and subsequent contaminant behaviour is largely unknown. Experimental work with ryegrass (Lolium perenne) is presented, exploring the impact of the physical presence of an LNAPL (mineral oil) on plant growth, root distribution and oil removal. The presence of the oil was found to significantly impact root biomass and distribution, leading to zones of increased root growth alongside decreased shoot growth. Significant removal of the LNAPL was noted in both hydroponic conditions and planted soil.


Phytoremediation Ryegrass Mineral oil Non-aqueous phase liquids 


  1. 1.
    Ucisik AS, Trapp S (2008) Uptake, removal, accumulation and phytotoxicity of 4-chlorophenol in willow trees. Arch Environ Contam Toxicol 54:619–627CrossRefGoogle Scholar
  2. 2.
    Zhu L, Zhang M (2008) Effect of rhamnolipids on the uptake of PAHs by ryegrass. Environ Pollut 156:46–52CrossRefGoogle Scholar
  3. 3.
    Huang XD, El-Alawi Y, Gurska J et al (2005) A multi-process phytoremediation system for decontamination of persistent total petroleum hydrocarbons (TPHs) from soils. Microchem J 81:139–147CrossRefGoogle Scholar
  4. 4.
    Gurska J, Wang WX, Gerhardt KE et al (2009) Three year field test of a plant growth promoting rhizobacteria enhanced phytoremediation system at a land farm for treatment of hydrocarbon waste. Environ Sci Technol 43:4472–4479CrossRefGoogle Scholar
  5. 5.
    Afzal M, Yousaf S, Reichenauer TG et al (2011) Soil type affects plant colonization, activity and catabolic gene expression of inoculated bacterial strains during phytoremediation of diesel. J Hazard Mater 186:1568–1575CrossRefGoogle Scholar
  6. 6.
    Rezek J, Wiesche CID, Mackova M et al (2008) The effect of ryegrass (Lolium perenne) on decrease of PAH content in long term contaminated soil. Chemosphere 70:1603–1608CrossRefGoogle Scholar
  7. 7.
    Zhang Q, Goplen B, Vanderhoof S et al (1996) Fate and effect of trichloroethylene as nonaqueous phase liquid in chambers with alfalfa. In: Proceedings of 26th annual biochemical engineering symposium, Kansas State University, USGoogle Scholar
  8. 8.
    Soga K, Page JWE, Illangasekare TH (2004) A review of NAPL source zone remediation efficiency and the mass flux approach. J Hazard Mater 110:13–27CrossRefGoogle Scholar
  9. 9.
    Kechavarzi C, Pettersson K, Leeds-Harrison P et al (2007) Root establishment of perennial ryegrass (L. perenne) in diesel contaminated subsurface soil layers. Environ Pollut 145:68–74CrossRefGoogle Scholar
  10. 10.
    British Standards Institution (1990) Methods of test for soils for civil engineering purposes. BS 1377:1990Google Scholar
  11. 11.
    Al-Ansary M, Al-Tabbaa A (2007) Stabilisation/solidification of synthetic petroleum drill cuttings. J Hazard Mater 141:410–421CrossRefGoogle Scholar
  12. 12.
    Franco JA, Banon S, Vicente MJ et al (2011) Root development in horticultural plants grown under abiotic stress conditions - a review. J Horticult Sci Biotechnol 86:543–556CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Sunday Oniosun
    • 1
  • Michael Harbottle
    • 1
    Email author
  • Snehasis Tripathy
    • 1
  • Peter Cleall
    • 1
  1. 1.School of EngineeringCardiff UniversityCardiffUK

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