Synchrotron-Radiation Vacuum-Ultraviolet Circular-Dichroism Spectroscopy for Characterizing the Structure of Saccharides
Circular-dichroism (CD) spectroscopy is a powerful tool for analyzing the structures of chiral molecules and biomolecules. The development of CD instruments using synchrotron radiation has greatly expanded the utility of this method by extending the spectra to the vacuum-ultraviolet (VUV) region below 190 nm and thereby yielding information that is unobtainable by conventional CD instruments. This technique is especially advantageous for monitoring the structure of saccharides that contain hydroxy and acetal groups with high-energy transitions in the VUV region. Combining VUVCD spectra with theoretical calculations provides new insight into the contributions of anomeric hydroxy groups and rotational isomers of hydroxymethyl groups to the dynamics, intramolecular hydrogen bonds, and hydration of saccharides in aqueous solution.
KeywordsCircular dichroism Glycoprotein Glycosaminoglycan Hydration Intramolecular hydrogen bond Molecular dynamics simulation Saccharide Solution structure Structural dynamics Synchrotron radiation Time-dependent density functional theory Vacuum ultraviolet
- methyl α-d-Glc
- methyl β-d-Glc
nuclear magnetic resonance
time-dependent density functional theory
vacuum-ultraviolet circular dichroism
The authors sincerely thank Professor Masaki Taniguchi and staff members of Hiroshima Synchrotron Radiation Center, and Dr. Tomoyuki Fukazawa of JASCO Corporation for constructing the SR-VUVCD spectrometer. The authors are indebted to many collaborators for their helpful technical assistance and discussions. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan (No. 15K07028 to K.M.) and by a grant from Kurata Memorial Hitachi Science and Technology Foundation.
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