Synchrotron-Radiation Vacuum-Ultraviolet Circular-Dichroism Spectroscopy for Characterizing the Structure of Saccharides

  • Koichi MatsuoEmail author
  • Kunihiko Gekko
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1104)


Circular-dichroism (CD) spectroscopy is a powerful tool for analyzing the structures of chiral molecules and biomolecules. The development of CD instruments using synchrotron radiation has greatly expanded the utility of this method by extending the spectra to the vacuum-ultraviolet (VUV) region below 190 nm and thereby yielding information that is unobtainable by conventional CD instruments. This technique is especially advantageous for monitoring the structure of saccharides that contain hydroxy and acetal groups with high-energy transitions in the VUV region. Combining VUVCD spectra with theoretical calculations provides new insight into the contributions of anomeric hydroxy groups and rotational isomers of hydroxymethyl groups to the dynamics, intramolecular hydrogen bonds, and hydration of saccharides in aqueous solution.


Circular dichroism Glycoprotein Glycosaminoglycan Hydration Intramolecular hydrogen bond Molecular dynamics simulation Saccharide Solution structure Structural dynamics Synchrotron radiation Time-dependent density functional theory Vacuum ultraviolet 



α1-acid glycoprotein


circular dichroism






molecular dynamics

methyl α-d-Glc

methyl α-d-glucopyranoside

methyl β-d-Glc

methyl β-d-glucopyranoside


nuclear magnetic resonance


synchrotron radiation


time-dependent density functional theory






vacuum ultraviolet


vacuum-ultraviolet circular dichroism



The authors sincerely thank Professor Masaki Taniguchi and staff members of Hiroshima Synchrotron Radiation Center, and Dr. Tomoyuki Fukazawa of JASCO Corporation for constructing the SR-VUVCD spectrometer. The authors are indebted to many collaborators for their helpful technical assistance and discussions. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan (No. 15K07028 to K.M.) and by a grant from Kurata Memorial Hitachi Science and Technology Foundation.


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© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Hiroshima Synchrotron Radiation CenterHiroshima UniversityHigashi-Hiroshima, HiroshimaJapan

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