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Isomeric Separation and Characterisation of Glycoconjugates

  • Kathirvel Alagesan
  • Arun Everest-Dass
  • Daniel Kolarich
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1104)

Abstract

Individual monosaccharides can be linked in a variety of different combinations to form complex glycoconjugates. In contrast to DNA and proteins, glycoconjugate synthesis does not follow any template but is the consequence of the concerted action of various enzymes such as transferases and glycosidases. Thus, tools for glycoconjugate sequencing need to differentiate individual monosaccharide identity, linkage and anomericity to investigate and understand glycoconjugate function. In this chapter we provide a concise overview on the most commonly used and robust tools to separate and characterise glycoconjugate isomers.

Keywords

Glycomics Glycoproteomics N-glycan O-glycan Porous graphitized carbon PGC HILIC HPLC 

Abbreviation

CCS

Collision cross section

CE

Capillary electrophoresis

DNA

Deoxyribonucleic acid

DP

Degree of polymerisation

ESI

Electrospray ionisation

Gal

Galactose

GlcNAc

N-Acetylglucosamine

GSL

Glycosphingolipid

HILIC

Hydrophilic interaction chromatography

HPLC

High-performance liquid chromatography

IP

Ion pairing

mAb

Monoclonal antibody

MALDI

Matrix-assisted laser desorption/ionisation

MS

Mass spectrometry

NeuAc

N-Acetylneuraminic acid

NMR

Nuclear magnetic resonance

PGC

Porous graphitised carbon

RNA

Ribonucleic acid

RP

Reverse phase

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Kathirvel Alagesan
    • 1
  • Arun Everest-Dass
    • 1
  • Daniel Kolarich
    • 1
  1. 1.Institute for GlycomicsGriffith UniversitySouthportAustralia

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