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Pitfalls in Immunocytochemical Study Using Fine Needle Aspiration Samples

  • Junko MarutaEmail author
Chapter

Abstract

Immunostaining of histopathological specimen is carried out using formalin-fixed paraffin-embedded tissues with or without antigen retrieval. Meanwhile, immunostaining of fine needle aspiration (FNA) smear is usually carried out using 95% alcohol-fixed specimens. The immunostaining results obtained with histopathological specimens and those with cytological specimens may be discrepant or even contradictory due to the differences in fixatives, fixation methods, and/or antigen activation treatment. An automated immunostaining instrument in our hospital is used for the special care in fixation of cytological smears. For FNA smear, alcohol fixation is used, which is followed by further fixation with phosphate-buffered formalin solution and an antigen activation treatment. Useful immunostaining markers in diagnosis of thyroid tumors are shown in Table 57.1.

References

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    Maruta J, Hashimoto H, Yamashita H, et al. Value of thyroid specific peroxidase and Ki-67 stains in preoperative cytology for thyroid follicular tumors. Diagn Cytopathol. 2015;43(3):202–9.CrossRefGoogle Scholar
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    Brown GC, Tao LC. Restoration of broken cytology slides and creation of multiple slides from a single smear preparation. Acta Cytol. 1992;36(2):259–63.PubMedGoogle Scholar
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    Sherman ME, Jimena-Joseph D, Grang MD, et al. Immunostaining of small cytologic specimens. Facilitation with cell transfer. Acta Cytol. 1994;38(1):18–22.PubMedGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  1. 1.Noguchi Thyroid Clinic and Hospital FoundationOitaJapan

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