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Zebrafish Wnt/β-Catenin Signaling Reporters Facilitate Understanding of In Vivo Dynamic Regulation and Discovery of Therapeutic Agents

  • Tohru Ishitani
  • Juqi Zou
Chapter

Abstract

Wnt/β-catenin signaling plays multiple roles in embryogenesis, organogenesis, and adult tissue homeostasis, and its dysregulation is linked to numerous human diseases such as cancer. Although strict spatiotemporal regulation must support the multi-functionality of Wnt/β-catenin signaling, detailed mechanisms remain unclear. In addition, Wnt/β-catenin signaling is a potential drug target candidate and several inhibitors have been identified by in vitro screening, but none have yet been incorporated into clinical practice. Recent studies using reporter zebrafish lines have gradually improved our understanding of in vivo dynamic regulation of Wnt/β-catenin signaling and have facilitated the discovery of new chemicals that can reduce Wnt/β-catenin signaling and cancer cell viability with few side effects. Here, we describe several new mechanisms supporting the spatiotemporal regulation of Wnt/β-catenin signaling and new small molecule inhibitors, discovered using zebrafish reporters. In addition, we discuss the potential of zebrafish signaling reporters in both developmental biology and pharmaceutical sciences.

Keywords

Wnt/β-catenin signaling reporter modifier chemical inhibitor anti-cancer drug 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Lab of Integrated Signaling Systems, Department of Molecular Medicine, Institute for Molecular & Cellular RegulationGunma UniversityMaebashiJapan

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