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STR Typing and Available Kits

  • Pankaj Shrivastava
  • Hirak Ranjan Dash
  • R. K. Kumawat
  • Ankit Srivastava
  • Jahangir Imam
Chapter

Abstract

This chapter describes a time-scale development of short tandem repeats (STRs) and STR-based DNA technology used in forensic DNA analysis. The text describes the subsequent advancements in the development of the STR multiplex systems. This sequential development in STR-based multiplex systems has increased efficiency, sensitivity, and inhibitor tolerance with improved buffers. The development of different variants of STR-based multiplex systems utilizing different variants including autosomal, mini-, Y-, and X-STRs has increased the flexibility and ease of the forensic DNA analyst. With the availability of genetic analyzers, utilizing sixth dye has created new possibilities toward availability of expanded STR multiplex without affecting the requirement of input DNA for PCR. Rapid PCR and application of next-generation sequencing are also discussed along with the already validated capillary electrophoresis technology.

Keywords

STRs DNA analysis Y and X STRs Multiplex PCR Autosomal NGS 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Pankaj Shrivastava
    • 1
  • Hirak Ranjan Dash
    • 1
  • R. K. Kumawat
    • 2
  • Ankit Srivastava
    • 3
  • Jahangir Imam
    • 4
  1. 1.DNA Fingerprinting Unit, State Forensic Science LaboratorySagarIndia
  2. 2.State Forensic Science LaboratoryJaipurIndia
  3. 3.Dr. A.P.J. Abdul Kalam Institute of Forensic ScienceBundelkhand UniversityJhansiIndia
  4. 4.DNA Fingerprinting Unit, State Forensic Science Laboratory, Department of Home, Jail and Disaster ManagementGovernment of JharkhandRanchiIndia

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