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Growth and Differentiation of Dental Stem Cells of Apical Papilla on Polycaprolactone Scaffolds

  • Mohamed Jamal
  • Yaser Greish
  • Sami Chogle
  • Harold Goodis
  • Sherif M. Karam
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1077)

Abstract

Biodegradable scaffolds are useful tools in the field of tissue engineering and regenerative medicine. The aim of this study was to test the potential of the human stem cells of apical papilla (SCAP) to attach, proliferate and differentiate on a polycaprolactone (PCL)-based scaffolds. SCAP were extracted from the root apical papillae of freshly extracted immature premolar teeth by using enzymatic digestion. Porous PCL scaffolds were fabricated using particle leaching method and NaCl or mannitol as porogens. SCAP of passage 3 were seeded on non-porous and porous PCL scaffolds for up to 14 days. For control, cells were cultured on glass coverslips. Picogreen DNA quantification was used to assay for cell proliferation. Cell differentiation and development of calcification nodules were examined using scanning electron microscopy and alizarin red staining. SCAP showed a comparable attachment, growth and proliferation patterns on PCL scaffolds and coverslips. Cell proliferation was enhanced on mannitol scaffolds at all time points. Calcification nodules were detected in all PCL scaffolds while it was not present on glass coverslips. These nodules were detected on NaCl-scaffolds by day 7 and on mannitol and non-porous scaffolds by day 14. In conclusion, SCAP were able to attach, proliferate and differentiate on PCL scaffolds without using any inductive media, indicating their potential application for dental tissue regeneration.

Keywords

Polycaprolactone Stem cells of apical papilla Dental stem cells Calcification nodules Porous scaffolds Regenerative endodontics 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Mohamed Jamal
    • 1
    • 2
  • Yaser Greish
    • 3
  • Sami Chogle
    • 4
  • Harold Goodis
    • 5
  • Sherif M. Karam
    • 1
  1. 1.Department of Anatomy, College of Medicine and Health SciencesUAE UniversityAl-AinUnited Arab Emirates
  2. 2.Department of Endodontics, Hamdan Bin Mohamed College of Dental MedicineMohamed Bin Rashid University of Medicine and Health SciencesDubaiUnited Arab Emirates
  3. 3.Department of Chemistry, College of ScienceUAE UniversityAl-AinUnited Arab Emirates
  4. 4.Department of Endodontics, Henry M. Goldman School of Dental MedicineBoston UniversityBostonUSA
  5. 5.Department of Preventive & Restorative Dental Sciences, School of DentistryUniversity of CaliforniaSan FranciscoUSA

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