Silk Fibroin-Based Scaffold for Bone Tissue Engineering

  • Joo Hee Choi
  • Do Kyung Kim
  • Jeong Eun Song
  • Joaquim Miguel Oliveira
  • Rui Luis Reis
  • Gilson KhangEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1077)


Regeneration of diseased or damaged skeletal tissues is one of the challenge that needs to be solved. Although there have been many bone tissue engineering developed, scaffold-based tissue engineering complement the conventional treatment for large bone by completing biological and functional environment. Among many materials, silk fibroin (SF) is one of the favorable material for applications in bone tissue engineering scaffolding. SF is a fibrous protein mainly extracted from Bombyx mori. and spiders. SF has been used as a biomaterial for bone graft by its unique mechanical properties, controllable biodegradation rate and high biocompatibility. Moreover, SF can be processed using conventional and advanced biofabrication methods to form various scaffold types such as sponges, mats, hydrogels and films. This review discusses about recent application and advancement of SF as a biomaterial.


Silk fibroin Biomaterial Bone tissue engineering Bone regeneration Tissue engineering Scaffold 



Alkaline phosphatase


Adipose-derived stem cells


Broussonetia kazinoki


Bone marrow derived mesenchymal stem cells


Bone morphogenic protein-2


Demineralized bone matrix


3 dimensional


Extracellular matrix


Food and Drug Administration




Human mesenchymal stem cells




Mesenchymal stem cells


Micro computed tomography








Polyethylene glycole




Silk fibroin


Tricalcium phosphate


Tissue Engineering


Vascular endothelial growth factor



This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2017R1A2B3010270) and Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health &Welfare, South Korea (HI15C2996).


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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Joo Hee Choi
    • 1
  • Do Kyung Kim
    • 1
  • Jeong Eun Song
    • 1
  • Joaquim Miguel Oliveira
    • 2
    • 3
    • 4
  • Rui Luis Reis
    • 2
    • 3
    • 4
  • Gilson Khang
    • 1
    Email author
  1. 1.Department of BIN Convergence TechnologyChonbuk National UniversityJeonju-siSouth Korea
  2. 2.Department of PolymerNano Science & Technology and Polymer Fusion Research CenterChonbuk National UniversityJeonju-siSouth Korea
  3. 3.ICVS/3B’s – PT Government Associated LaboratoryBraga/GuimarãesPortugal
  4. 4.The Discoveries Centre for Regenerative and Precision MedicineHeadquarters at University of Minho, AveparkGuimarãesPortugal

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