Technology Platforms for Bioanalysis of Quorum Sensing System and Exploiting It as Biomonitors and Biosensors

  • Santoshkumar N. PatilEmail author
  • Swapnil C. Kamble


Quorum sensing (QS) is a bacterial signaling phenomenon wherein bacteria regulate gene expression as per the concentration of signaling molecule. In the microbial milieu, bacteria use QS to sense their immediate environment and in-turn adjust QS genes. Our knowledge of this continuous process of biosensing and biomonitoring of QS signaling molecule and QS circuits has evolved over a period of time. Herein, we attempt to follow impact of newer bioanalysis techniques in understanding this QS phenomenon based on only recent technology platforms. Some of the technology platforms are at proof of concept stage wherein feasibility for QS studies is being demonstrated. We attempt to understand the enormous possibilities/potential these technologies withhold. Advancements in QS systems led researchers to attempt potential application of QS systems itself as technology platform. In this book chapter, few specific applications of QS system towards biosensing and biomonitoring are explored and covers above mentioned topics in four sections: (a) advanced structural based techniques involved in QS study (b) advanced biosensing and biomonitoring technologies (c) microarray technology (d) QS technologies for biosensing and biomonitoring activity. Specific examples are elaborated in details and for comprehensive reading on technology platform reader could refer to the references. In summary, advanced technology towards bioanalysis and applications of QS itself as biosensing and biomonitoring technology are discussed. The critical analysis, current trends, potential technology applications and the path forward are touched upon in key opinion and conclusion.


Bioanalysis Quorum sensing Biomonitors Biosensors Technology platform Biofilms Quorum sensing circuits 



Acylhomoserine lactone




Autoinducer -2


Cis-2-decenoic acid


Confocal Raman microspectroscopy


Desorption electrospray ionization


Extracellular polymeric substances


Gamma-aminobutyric acid


Generally regarded as safe


Imaging mass spectrometry




Matrix-assisted laser desorption–ionization


Mass spectroscopy


Nuclear magnetic resonance


N-(3-oxooctanoyl) homoserine lactone


N-(3-oxododecanoyl) homoserine lactone




Quorum quenching


Quorum sensing


Restriction endonuclease


Sporulation delaying protein


Scanning electrochemical microscopy


Secondary-ion mass spectrometry


Sporulation killing factor


Square wave voltammetry


Time of flight




World Health Organization



Authors would like to thank Sai Life Sciences management and leadership team and Dean, Faculty of Technology, Savitribai Phule Pune University for approval and support. A special thanks to Dr. Sarma, VP, Medicinal Chemistry, Sai Life Sciences, Pune, India for support and encouragement provided during writing this chapter and Dr. Joyita Sarkar for proof-reading. We also thank Prof. Kalia, Emeritus Scientist, CSIR-Institute of Genomics and Integrative Biology, for guidance and an invitation to write book chapter.


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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Sai Life Sciences Ltd, BTS, Chrysalis Enclave, International Bio Tech ParkHinjawadi Phase II, PuneIndia
  2. 2.Department of TechnologySavitribai Phule Pune UniversityPuneIndia

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