Tissue Culture in Mulberry (Morus spp.) Intending Genetic Improvement, Micropropagation and Secondary Metabolite Production: A Review on Current Status and Future Prospects

  • Tanmoy Sarkar
  • Thallapally Mogili
  • S. Gandhi Doss
  • Vankadara Sivaprasad


Mulberry (Morus spp.) is a woody, perennial, highly heterozygous, fast-growing plant and grown mainly for its foliage worldwide under various agroclimatic zones (tropical, subtropical and temperate) of Asia, Africa and the Americas. Mulberry leaves are the sole food source for monophagous and domesticated mulberry silkworm, Bombyx mori L. Moreover, mulberry fruits are fleshy, succulent and delicious berries. The fruits are low in calories and contain health-promoting phytonutrients such as polyphenols, minerals and vitamins having medicinal importance for its antioxidant, antitumor, neuroprotective activities and hypo-lipidemic/macrophage activating effects. Genetic improvement of mulberry is mainly aimed for improving productivity and quality of leaf for silk production. Conventional plant breeding techniques including tissue culture and molecular biology methods are employed in mulberry genetic improvement programmes to develop varieties for improved leaf productivity and biotic/abiotic stress tolerance. This review focuses on various tissue culture approaches such as in vitro regeneration, micropropagation, genetic transformation, somaclonal variation, in vitro selection, suspension culture, and much more which often supplement the traditional breeding methods. Further, characterization and production of secondary metabolites from mulberry tissues through suspension culture which are becoming a blooming option for commercial exploration of bioactive compounds have been discussed.


Organogenesis Somatic embryogenesis Somaclonal variation Double haploid Somatic hybrid Transgenic mulberry Secondary metabolite 


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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Tanmoy Sarkar
    • 1
  • Thallapally Mogili
    • 1
  • S. Gandhi Doss
    • 1
  • Vankadara Sivaprasad
    • 1
  1. 1.Central Sericultural Research & Training Institute (CSRTI)MysuruIndia

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