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Emergence of Bias During the Synthesis and Amplification of cDNA for scRNA-seq

  • Qiankun Luo
  • Hui Zhang
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1068)

Abstract

The advent of single-cell omics technology has promoted our understanding of the genomic, epigenomic, and transcriptomic heterogeneity in individual cells. Compared to traditional sequencing studies using bulk cells, single-cell transcriptome technology is naturally more dynamic for in depth analysis of genomic variation resulting from cell division and is useful in unraveling the regulatory mechanisms of gene networks in many diseases. However, there are still some limitations of current single-cell RNA sequencing (scRNA-seq) protocols. Biases that arise during the RNA reverse transcription and cDNA pre-amplification steps are the most common problems and play pivotal roles in limiting the quantitative accuracy of scRNA-seq. In this review, we will describe how these biases emerge and impact scRNA-seq protocols. Moreover, we will introduce several current and convenient modified scRNA-seq methods that allow for bias to be decreased and estimated.

Keywords

Amplification Single-cell Transcriptomic Technical noise 

Notes

Conflicts of Interest Statement

Qiankun Luo and Hui Zhang have no conflicts of interest.

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Qiankun Luo
    • 1
  • Hui Zhang
    • 2
  1. 1.Department of General Surgery, Second Affiliated HospitalZhengzhou UniversityZhengzhouChina
  2. 2.Department of Science Research and Discipline ConstructionHenan Provincial People’s HospitalJinshui District, ZhengzhouChina

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