In Silico Biochemical Pathways for Bacterial Metabolite Synthesis

Abstract

The search of an alternative is always a major concern of human for sustaining life effectively. The green flora described by the agriculture is the source of many such life attaining processes and products that are essential for human population. In addition to this, the constantly growing number of Homo sapiens has to be fed with increase yield of agriculture products. To meet the demands of growing population and relieve the pressure of yield, the use of fertilizers comes into action, while the constant use of chemical fertilizers has deteriorated the heath of soil, environment, and human collectively calling “phytobiome.” Thus, the urge of finding alternatives to replace the toxic chemical fertilizers has given a way to search exhaustively the naturally occurring microbiomes for their beneficial effect on the agri-flora. Moreover, the available advancements in the computational and system-level approaches with omics data have provided us the genomes and also genome-level metabolic models for many beneficial/effective bacteria. The naturally synthesized metabolites (primary and secondary) can be easily exploited nowadays for any intended use in the fields as inoculants or bio fertilizers. In addition the available kinetic model has paved the way to commercially synthesize desired metabolite (through amendments in pathway either genetic or environmental) on large scale as biofuels, etc. Despite of these advances, several challenges still coexist with approaches that have to be exploited in the near future. Some of the challenges have been discussed in present work with a brief account of in silico kinetic models available.