Isolation, Identification and Characteristics of an Efficient PCBs-Degrading Strain
Abstract
An efficient polychlorinated biphenyls (PCBs)-degrading bacteria named ZW was cultivated and isolated from soil suffering from long-term PCBs contamination. The strain was identified as Pseudomonas aeruginosa through 16S rDNA sequence analysis. Experiments were conducted to study the degradation characteristics of the bacteria for PCBs. The study revealed that the strain ZW could grow with PCB77 as the only carbon source and its best 7-day degradation rate in 2 mL culture (OD600 = 1.0) was 50.1%, with the PCB77 concentration at 1.0 mg L−1, pH 7.5, 30 °C and rotating speed 150 r min−1 in a Thermostatic Vibrating Incubator. The influence of additives, including four organic matters and five heavy metals, on the degradation rate was also studied. It was indicated that the degradation rates changed to 65.2, 59.58, 52.3 and 39.8%, respectively, when the same concentration (i.e. 1.0 mg L−1) of Tween-80 or biphenyl or phthalic acid or benzoic acid was introduced. The presence of Cd2+, Cu2+, Ni2+, Cr6+ and Pb2+ had inhibiting effect on the biodegradation rate in a descending order Cd2+ > Cu2+ > Cr6+ > Ni2+ > Pb2+ with the same heave metal ion concentration, and the higher the concentration, the more significant the inhibitory effect was. As the number of chlorine atoms in PCB congeners (PCB18, PCB77, PCB101) increased, the degradation rate changed to 89.6, 49.6 and 23.5%, respectively. The toxicity of PCBs biodegradable system decreased over time and the drop rate showed negative correlation with the number of chlorine atoms in PCBs.
Keywords
Polychlorinated Biphenyls-degrading bacteria Degradation characteristics Heavy metals BiotoxicityReferences
- 1.S. Lucile, P. Sophie, F. Mathieu, et al., Autochthonous ascomycetes in depollution of polychlorinated biphenyls contaminated soil and sediment. Chemosphere 110, 62–69 (2014)Google Scholar
- 2.T. Zhang, S.J. Chen, N. Li, et al., Current situation and ecological risk of typical pops in the surface sediments of Taizhou water system. Res. Environ. Sci. 27(12), 1540–1548 (2014)Google Scholar
- 3.Y. Yang, Z.Q. Wang, Q. Wang, et al., Characteristics and environment risk assessment of PCBs in multi-media environment of the electronic waste dismantling venues. Asian J. Ecotoxicol. 9(1), 133–144 (2014)Google Scholar
- 4.K. Furukawa, H. Fujihara, Microbial Degradation of polychlorinated biphenyls: Biochemical and molecular features. Journal of Bioscience and Bioengineering, 105(5) (2008) 433–449.Google Scholar
- 5.O.I. Matthew, K.R. Gary, A.A. Sunday, Degradation and mineralization of 2-chloro-, 3-chloro- and 4-chlorobiphenyl by a newly characterized natural bacterial strain isolated from an electrical transformer fluid-contaminated soil. J. Environ. Sci. 20 1250–1257 (2008)Google Scholar
- 6.B.A. Rasulov, A.A. Kim, A. Lorenz, et al., Biodegradation of tritium labeled polychlorinated biphenyls (PCBs) by local salt tolerant mesophylic bacillus strains. J. Environ. Protect. 1, 420–425 (2010)Google Scholar
- 7.T. Chen, T. Ying, M.L. Yong, et al., Potential for biodegradation of polychlorinated biphenyls (PCBs) by Sinorhizobium meliloti. J. Hazard. Mat. 186(2–3), 1438–1444 (2011)Google Scholar
- 8.S.Y. Shi, L. Feng, J. Gong, Isolation and degradation characteristics of PCB-degrading strain. Environ. Sci. 33(10), 3627–3633 (2012)Google Scholar
- 9.M. Liu, B. Huang, X.H. Bi, et al., Heavy metals and organic compounds contamination in soil from an e-waste region in South China. Environ. Sci. Processes Impacts 15, 919–929 (2013)Google Scholar
- 10.T.R. Sandrin, D.R. Hoffman, Bioremediation of organic and metal co-contaminated environment: effects of metal toxicity, speciation, and bioavailability on biodegradation. Environ. Bioremediat. Technol. 1–34 (2007)Google Scholar
- 11.T. Valentina, V.S. Catefina, F. Stefano, et al., Tolerance of Pseudomonas pseudoalcaligenes KF707 to metals, polychlorobiphenyls and chlorobenzoates: effects on chemotaxis, biofilm-and planktonic-grown cells. FEMS Microbiol. Ecol. 74, 291–301 (2010)Google Scholar
- 12.B.L. Wang, X.L. Zhang, Q. Zhang, et al., Determination of 39 polychlorinated biphenyls in indoor dust using ultrasonic extraction and gas chromatography-tandem mass spectrometry. Chin. J. Chromatogr. 32(1), 74–80 (2014)Google Scholar
- 13.Y.F. Shi, Y.Q. Cai, H.J. Yu, et al, Identification of polychlorinated biphenyls in water products by gas chromatography-mass spectrometry. Chin. J. Anal. Chem. 42(11), 1640–1645 (2014)Google Scholar
- 14.D.M. Su, Z.G. Miao, X.P. Song, et al., Optimization of liquid growth conditions and determination of growth curves for bartonella species. Microbiol. China 39(11), 1695–1702 (2012)Google Scholar
- 15.F.H. Li, L. Xu, T.H. Zhang, et al., Degradation characteristics and fermentation conditions optimization of a PCBs-degrading strain. Microbiol. China, 41(7), 1299–1307 (2014)Google Scholar
- 16.S.C. Xiong, H. Yin, B.Y. He, et al., Enzymatic degradation of decabromodiphenyl ether by white rot fungi. Environ. Chem. 31(5), 615–619 (2012)Google Scholar
- 17.Y.M. Cao, L. Xu, L.Y. Jia, Analysis of PCBs degradation abilities of biphenyl dioxygenase derived from Enterobacter sp. LY402 by molecular simulation. New Biotechnol. 29(1), 90–98 (2011)Google Scholar
- 18.Y. Xu, G. D. Sun, J.H. Jin, et al., Successful bioremediation of an aged and heavily contaminated soil using a microbial plant combination strategy. J. Hazard. Mat. 264, 430–438 (2014)Google Scholar