Seedling Bio-priming with Trichoderma spp. Enhances Nitrogen Use Efficiency in Rice

  • Preeti Priya
  • Kartikay Bisen
  • Amitava Rakshit
  • H. B. Singh


Present study was undertaken to investigate the effect of seedling bio-priming with Trichoderma harzianum (ATCC No. PTA-3701), varied N fertilizer doses (100%, 75%, 50%, and 25% RDN), and different soil type on rice plant under greenhouse condition. Results showed that the plant growth, chlorophyll content, nutrient use efficiency, and grain N content were significantly increased in various treatments. Significantly higher growth promotion, chlorophyll content, nutrient use efficiency, and grain N content were found with recommended dose of fertilizer (RDF) NPK @ 120-60-60 kg ha−1 followed by seedling treated with T. harzianum + 3/4th N and RDF of PK. Seedling bio-priming with T. harzianum also enhanced the plant growth and nutrient use efficiency in rice plant. Agronomic use efficiency and physiological use efficiency were maximum in alluvial soil as compared to black soil and red soil. Results indicated the suitability of seedling bio-priming with T. harzianum for better plant growth and nutrient use efficiency in rice plants.


Bio-priming Trichoderma Nitrogen use efficiency (NUE) 


  1. Adesemoye AO, Torbert HA, Kloepper JW (2009) Plant growth-promoting rhizobacteria allow reduced application rates of chemical fertilizers. Microb Ecol 58:921–929CrossRefPubMedGoogle Scholar
  2. Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1CrossRefPubMedPubMedCentralGoogle Scholar
  3. Bisen K, Keswani C, Mishra S, Saxena A, Rakshit A, Singh HB (2015) Unrealized potential of seed biopriming for versatile agriculture. In: Rakshit A, Singh HB, Sen A (eds) Nutrient use efficiency: from basics to advances. Springer, New Delhi, pp 193–206CrossRefGoogle Scholar
  4. Hoang VN, Alauddin M (2010) Assessing the eco-environmental performance of agricultural production in OECD countries: the use of nitrogen flows and balance. Nutr Cycl Agroecosyst 87:353–336CrossRefGoogle Scholar
  5. Keswani C, Mishra S, Sarma BK, Singh SP, Singh HB (2014) Unraveling the efficient application of secondary metabolites of various Trichoderma. Appl Microbiol Biotechnol 98:533–544CrossRefPubMedGoogle Scholar
  6. Martínez-Medina A, Martınez-Medina RA, Jose AP (2011) Performance of a Trichoderma harzianum bentonite–vermiculite formulation against fusarium wilt in seedling nursery melon plants. Hortscience 44:2025–2027Google Scholar
  7. Meena SK, Rakshit A, Meena VS (2016) Effect of seed bio-priming and N doses under varied soil type on nitrogen use efficiency (NUE) of wheat (Triticum aestivum L.) under greenhouse conditions. Biocatalyst Agric Biotechnol 6:668–675Google Scholar
  8. Mehetre ST, Mukherjee PK (2015) Trichoderma improves nutrient use efficiency in crop plants. In: Rakshit A, Singh HB, Sen A (eds) Nutrient use efficiency: from basics to advances. Springer, India, pp 173–180CrossRefGoogle Scholar
  9. Mulvaney RL, Khan SA, Ellsworth TR (2009) Synthetic nitrogen depleted soil nitrogen: a global dilemma for sustainable cereal production. J Environ Qual 38:2295–2314CrossRefPubMedGoogle Scholar
  10. Murphy PNC, O’Connell K, Watson S, Watson CJ, Humphreys J (2013) Seasonality of nitrogen uptake, apparent recovery of fertilizer nitrogen and background nitrogen supply in two Irish grassland soils. Ir J Agric Food Res 52:17–38Google Scholar
  11. Peng S, Garcia FV, Laza RC, Sanico AL, Visperas RM, Cassman KG (1996) Increased N- se efficiency using a chlorophyll meter on high-yielding irrigated rice. Field Crop Res 47:243–252CrossRefGoogle Scholar
  12. Rakshit A, Singh HB, Sen A (2015a) Nutrient use efficiency: from basics to advances. Springer, New DelhiCrossRefGoogle Scholar
  13. Rakshit A, Sunita K, Pal S, Singh A, Singh HB (2015b) Bio-priming mediated nutrient use efficiency of crop species. In: Rakshit A, Singh HB, Sen A (eds) Nutrient use efficiency: from basics to advances. Springer, New Delhi, pp 181–191CrossRefGoogle Scholar
  14. Raun WR, Johnson GV (1999) Improving nitrogen use efficiency for cereal production. Agron J 91:357–363CrossRefGoogle Scholar
  15. Singh HB (2016) Seed biopriming: a comprehensive approach towards agricultural sustainability. Indian Phytopathol 69:203–209Google Scholar
  16. Singh SP, Singh HB (2011) Effect of consortium of Trichoderma harzianum isolates on growth attributes and Sclerotinia sclerotiorum rot of brinjal. Veg Sci 39:144–148Google Scholar
  17. Tilman D, Cassman KG, Matson PA, Naylor R, Polasky S (2002) Agricultural sustainability and intensive production practices. Nature 418:671–677CrossRefGoogle Scholar
  18. Tripathi P, Singh PC, Mishra A, Chaudhry V, Mishra S, Tripathi RD, Nautiyal CS (2013) Trichoderma inoculation ameliorates arsenic induced phytotoxic changes in gene expression and stem anatomy of chickpea (Cicer arietinum). Ecotoxicol Environ Saf 89:8–14CrossRefPubMedGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Preeti Priya
    • 1
  • Kartikay Bisen
    • 2
  • Amitava Rakshit
    • 1
  • H. B. Singh
    • 2
  1. 1.Department of Soil Science and Agricultural Chemistry, Institute of Agricultural SciencesBHUVaranasiIndia
  2. 2.Department of Mycology and Plant Pathology, Institute of Agricultural SciencesBHUVaranasiIndia

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