Role of SNP-Mediated Nitric Oxide Priming in Conferring Low Temperature Tolerance in Wheat Genotype (Triticum aestivum L.): A Case Study in Indian Northern Plains
Wheat is an economically important cereal crop grown in the vast stretch along the northern, western and part of Central Indian plains serving as livelihood tool for more than half of agrarian population of the country. The crop being grown in rabi (mid-November–mid-April) faces multifaceted abiotic stress threats among which low temperature stress being one of them. Nitric oxide has been well documented to counter many of these threats as also low temperature stress. In this context, a study was conducted to evaluate the effect of nitric oxide priming on certain morphophysiological and biochemical parameters in wheat genotype HD-2329 facing low temperature regime in the laboratory of the Department of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu University. The several parameters under observations were per cent germination, germination rate, shoot length, root length, α-amylase activity and soluble sugar content both under normal (25 °C) and low temperature (5 °C) conditions. Seed priming with sodium nitroprusside (SNP, a nitric oxide donor) at 100 μM concentration resulted in enhanced performance of these parameters as compared to non-primed seeds both under normal and low temperature, but the effect was more pronounced at low temperature regime. Hence, it was concluded that NO priming of wheat seeds had a statistical significance in conferring low temperature tolerance in the crop, thus making it a fit priming model in a climate resilient era.
KeywordsNO priming Low temperature stress Wheat
The authors would like to acknowledge the cooperation obtained from the technical staff of the Department of Plant Physiology, I. Ag. Sc. BHU for smooth conduct of the research work.
- Ahmad MA, Sushmita, Prakash P (2015) Role of nitric oxide priming on germination attributes in wheat (Triticum aestivum L.) under low temperature. Environ Ecol 33:1245–1248Google Scholar
- Amooaghaie R (2011) The effect of hydro- and osmopriming on alfalfa seed germination and antioxidant defense under high salt concentration stress. Afr J Biotechnol 33:6269–6275Google Scholar
- Anonymous. (2016) State of Indian Agriculture (2015–16) Report, Ministry of Agriculture & Farmer’s Welfare, Goverment of IndiaGoogle Scholar
- Chen F, Wang F, Sun H, Cai Y, Mao W, Zhang G, Vincze E, Wu F (2010) Genotype-dependent effect of exogenous nitric oxide on Cd-induced changes in antioxidative metabolism, ultrastructure, and photosynthetic performance in barley seedlings (Hordeum vulgare). J Plant Growth Regul 29:394–408CrossRefGoogle Scholar
- Panse VG, Sukhatme PV (1967) Statistical methods for agricultural workers. ICAR Publication New Delhi pp 167–174Google Scholar