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Biphasic Rheology of Different Artificial Degenerated Intervertebral Discs

  • Mohammad Nikkhoo
  • Romina Kargar
  • Kinda Khalaf
Conference paper
Part of the IFMBE Proceedings book series (IFMBE, volume 68/2)

Abstract

Simulation of the intervertebral disc (IVD) degeneration in animal models is of great interest towards exploration and evaluation of potential regenerative therapeutics. Hence, the objective of this study was to investigate the biphaisc response of the IVD for different artificial degeneration models. Fifty motion segments were dissected from juvenile sheep lumbar spines. The specimens were assigned equally into 5 groups (i.e., (1) intact (I), (2) punctured with a 16-G needle (P), (3) punctured with a 16-G needle combined with fatigue loading (PF), (4) denatured by injecting 0.5 ml 0.25% trypsin solution (T), and (5) denatured by injecting 0.5 ml 0.25% trypsin solution combined with fatigue loading (TF)). Specimens were mounted in a chamber filled with phosphate buffered saline and underwent a stress-relaxation test. Based on linear biphasic theory, the aggregate modulus (HA) and permeability (k) were extracted. Aggregate modulus decreased in P and T discs but increased in PF discs as compared to intact ones. The difference of the aggregate modulus between TF and intact discs was not significant. Permeability decreased in T, PF and TF discs. The permeability of both PF and TF discs was significantly lower than F and T discs, respectively. It is concluded that proposed artificial degeneration models can be used to investigate potential regenerative therapeutics.

Keywords

Intervertebral disc Degeneration Biphasic theory Biomechanics 

Notes

Conflict of Interest

The authors declare that this study research was conducted in the absence of any commercial or financial affiliations that could be construed as a potential conflict of interest.

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  1. 1.Department of Biomedical Engineering, Science and Research BranchIslamic Azad UniversityTehranIran
  2. 2.Department of Biomedical EngineeringKhalifa University of Science and TechnologyAbu DhabiUnited Arab Emirates

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