Hepatoprotective Action of Thymoquinone

  • Amaj A. Laskar


Nigella sativa (black cumin) seeds and its principal bioactive component, thymoquinone (TQ), have been used for centuries in folk medicine because of their exceptional healing power to a wide range of diseases. Nigella sativa plant has been regarded as nature’s miracle herb. The black cumin seeds have been used for the treatment of wide range of diseases including diabetes, infections, skin diseases, hypertension, cancer, asthma, gastric ulcers, liver diseases, etc. and are consumed regularly for the general well-being. The pharmacological properties possessed by TQ are anticancer, antidiabetic, antioxidant, anti-inflammatory, antimicrobial, gastroprotective, immunomodulatory, cytoprotective, hepatoprotective activity, etc. The hepatoprotective activity of TQ has been studied extensively in many in vitro and in vivo studies. It has been found to be an excellent natural protective agent against hepatotoxicity induced by several chemicals and drug molecules and the liver injury and liver diseases in experimental animal models. The antioxidant capacity of TQ was found to play a major role in the hepatoprotection. Therefore, TQ is a potential natural remedy to protect the liver from many toxic agents and drugs and for the treatment of liver diseases.


  1. Abd El-Ghany RM, Sharaf NM, Kassem LA et al (2009) Thymoquinone triggers anti-apoptotic signaling targeting death ligand and apoptotic regulators in a model of hepatic ischemia reperfusion injury. Drug Discov Ther 3:296–306PubMedGoogle Scholar
  2. Abd-Elbaset M, Arafa El-Shamima A, El Sherbiny GA et al (2017) Thymoquinone mitigate ischemia-reperfusion-induced liver injury in rats: a pivotal role of nitric oxide signaling pathway. Naunyn Schmiedeberg’s Arch Pharmacol 390:69–76CrossRefGoogle Scholar
  3. Abdel-Wahab WM (2014) Thymoquinone attenuates toxicity and oxidative stress induced by bisphenol a in liver of male rats. Pak J Biol Sci 17:1152–1160CrossRefGoogle Scholar
  4. Ahmad A, Husain A, Mujeeb M et al (2013) A review on therapeutic potential of Nigella sativa: a miracle herb. Asian Pac J Trop Biomed 3:337–352CrossRefGoogle Scholar
  5. Al-Bukhari MI (1976) Division 71 on medicine. In: Al-Bukhari S (ed) The collection of authentic sayings of Prophet Mohammad (peace be upon him), 2nd edn. Hilal Yayinlari, AnkaraGoogle Scholar
  6. Alenzi FQ, El-Bolkiny Yel S, Salem ML (2010) Protective effects of Nigella sativa oil and thymoquinone against toxicity induced by the anticancer drug cyclophosphamide. Br J Biomed Sci 67:20–28CrossRefGoogle Scholar
  7. Ali BH, Blunden G (2003) Pharmacological and toxicological properties of Nigella sativa. Phytother Res 17:299–305CrossRefGoogle Scholar
  8. Alsaif MA (2007) Effect of thymoquinone on ethanol-induced hepatotoxicity in wistar rats. J Med Sci 7:1164–1170CrossRefGoogle Scholar
  9. Awad AS, Abd Al Haleem EN, El-Bakly WM et al (2016) Thymoquinone alleviates nonalcoholic fatty liver disease in rats via suppression of oxidative stress, inflammation, apoptosis. Naunyn Schmiedeberg’s Arch Pharmacol 389:381–391CrossRefGoogle Scholar
  10. Aycan IO, Tufek A, Tokgoz O et al (2014) Thymoquinone treatment against acetaminophen-induced hepatotoxicity in rats. Int J Surg 12:213–218CrossRefGoogle Scholar
  11. Badary OA, Taha RA, Gamal el-Din AM et al (2003) Thymoquinone is a potent superoxide anion scavenger. Drug Chem Toxicol 26:87–98CrossRefGoogle Scholar
  12. Daba MH, Abdel-Rahman MS (1998) Hepatoprotective activity of thymoquinone in isolated rat hepatocytes. Toxicol Lett 95:23–29CrossRefGoogle Scholar
  13. Darakhshan S, Bidmeshki Pour A, Hosseinzadeh Colagar A et al (2015) Thymoquinone and its therapeutic potentials. Pharmacol Res 95–96:138–158CrossRefGoogle Scholar
  14. El-Far AH (2015) Thymoquinone anticancer discovery: possible mechanisms. Curr Drug Discov Technol 12:80–89CrossRefGoogle Scholar
  15. El-Far AH, El-Sayed YS, Shaheen HM (2016) Back to the natural herbs. EC Pharmacol Toxicol 2(6):273–276Google Scholar
  16. El-Sheikh AAK, Morsy MA, Abdalla AM et al (2015) Mechanisms of thymoquinone hepatorenal protection in methotrexate-induced toxicity in rats. Mediat Inflamm 2015:12CrossRefGoogle Scholar
  17. El-Tawil OS, Moussa SZ (2006) Antioxidant and hepatoprotective effects of thymoquinone against carbon tetrachloride-induced hepatotoxicity in isolated rat hepatocytes. J Egypt Soc. Toxicology 34:33–41Google Scholar
  18. Fararh KM, Shimizu Y, Shiina T et al (2005) Thymoquinone reduces hepatic glucose production in diabetic hamsters. Res Vet Sci 79:219–223CrossRefGoogle Scholar
  19. Goreja WG (2003) Black seed: nature’s miracle remedy. Amazing Herbs Press, New YorkGoogle Scholar
  20. Hassanein KMA, Al-Emam A, Radad K (2016) Prophylactic effects of thymoquinone against carbon tetrachloride-induced hepatic damage in Sprague-Dawley rats. J Appl Pharm Sci 6:167–171CrossRefGoogle Scholar
  21. Houghton PJ, Zarka R, de las Heras B et al (1995) Fixed oil of Nigella sativa and derived thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med 61:33–36CrossRefGoogle Scholar
  22. Jaswal A, Sinha N, Bhadauria M et al (2013) Therapeutic potential of thymoquinone against anti-tuberculosis drugs induced liver damage. Environ Toxicol Pharmacol 36:779–786CrossRefGoogle Scholar
  23. Kong LY, Li GP, Yang P et al (2015) Protective effect of thymoquinone on cholestatic rats with liver injury. Genet Mol Res 14:12247–12253CrossRefGoogle Scholar
  24. Laskar AA, Khan MA, Rahmani AH et al (2016) Thymoquinone, an active constituent of Nigella sativa seeds, binds with bilirubin and protects mice from hyperbilirubinemia and cyclophosphamide-induced hepatotoxicity. Biochimie 127:205–213CrossRefGoogle Scholar
  25. Laskar AA, Khan MA, Askari F et al (2017) Thymoquinone binds and activates human salivary aldehyde dehydrogenase: potential therapy for the mitigation of aldehyde toxicity and maintenance of oral health. Int J Biol Macromol 103:99–110CrossRefGoogle Scholar
  26. Mabrouk A, Bel Hadj Salah I, Chaieb W et al (2016) Protective effect of thymoquinone against lead-induced hepatic toxicity in rats. Environ Sci Pollut Res Int 23:12206–12215CrossRefGoogle Scholar
  27. Mady NI, Allam AF, Salem AI (2001) Evaluation of the addition of Nigella sativa oil triclabendazole therapy in the treatment of human fascioliasis. J Egypt. Pharmacol Exp Ther 20:807–827Google Scholar
  28. Mansour MA (2000) Protective effects of thymoquinone and desferrioxamine against hepatotoxicity of carbon tetrachloride in mice. Life Sci 66:2583–2591CrossRefGoogle Scholar
  29. Mansour MA, Ginawi OT, El-Hadiyah T et al (2001) Effects of volatile oil constituents of Nigella sativa on carbon tetrachloride-induced hepatotoxicity in mice: evidence for antioxidant effects of thymoquinone. Res Commun Mol Pathol Pharmacol 110:239–251PubMedGoogle Scholar
  30. Mansour MA, Nagi MN, El-Khatib AS et al (2002) Effects of thymoquinone on antioxidant enzyme activities, lipid peroxidation and DT-diaphorase in different tissues of mice: a possible mechanism of action. Cell Biochem Funct 20:143–151CrossRefGoogle Scholar
  31. Nagi MN, Alam K, Badary OA et al (1999) Thymoquinone protects against carbon tetrachloride hepatotoxicity in mice via an antioxidant mechanism. Biochem Mol Biol Int 47:153–159PubMedGoogle Scholar
  32. Pari L, Sankaranarayanan C (2009) Beneficial effects of thymoquinone on hepatic key enzymes in streptozotocin-nicotinamide induced diabetic rats. Life Sci 85:830–834CrossRefGoogle Scholar
  33. Raghunandhakumar S, Paramasivam A, Senthilraja S et al (2013) Thymoquinone inhibits cell proliferation through regulation of G1/S phase cell cycle transition in N-nitrosodiethylamine-induced experimental rat hepatocellular carcinoma. Toxicol Lett 223:60–72CrossRefGoogle Scholar
  34. Salem ML (2005) Immunomodulatory and therapeutic properties of the Nigella sativa L. seed. Int Immunopharmacol 5:1749–1770CrossRefGoogle Scholar
  35. Sayed-Ahmed MM, Aleisa AM, Al-Rejaie SS et al (2010) Thymoquinone attenuates diethylnitrosamine induction of hepatic carcinogenesis through antioxidant signaling. Oxidative Med Cell Longev 3:254–261CrossRefGoogle Scholar
  36. Srisailam DK, Vijayabhaskar K, Gowrishankar NL (2017) Hepatoprotective and antioxidant activity on n-butanol fraction of plants methanolic extract of Nigella sativa and Eclipta alba Linn. World. J Pharm Pharm Sci 6:1264–1280Google Scholar
  37. Suddek GM (2014) Protective role of thymoquinone against liver damage induced by tamoxifen in female rats. Can J Physiol Pharmacol 92:640–644CrossRefGoogle Scholar
  38. Zafeer MF, Waseem M, Chaudhary S et al (2012) Cadmium-induced hepatotoxicity and its abrogation by thymoquinone. J Biochem Mol Toxicol 26:199–205CrossRefGoogle Scholar

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© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Amaj A. Laskar
    • 1
  1. 1.Interdisciplinary Biotechnology UnitAligarh Muslim UniversityAligarhIndia

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