Applications of Fungal Nanobiotechnology in Drug Development

  • Kanti Bhooshan Pandey
  • Brahm Kumar Tiwari


Nanotechnology is gradually being incorporated into drug industry sector. This technology is used to overcome the problem of drug delivery through existing approaches; however the cost factors and implementation issues have restricted this field and increased the need for basic as well technological innovations. There is an increasing interest in the use of fungi in these processes since they have potential to generate eco-friendly and relatively rapid and clean metallic nanoparticles. Fungal nanobiotechnology (FNBT) has resulted in development of nanodrugs and novel diagnostic/analytical tools for therapy and prevention of many chronic diseases such as cancer, HIV infections, and kidney diseases. In present chapter applications of FNBT in targeted drug delivery, bio-sensing, and development of drugs with enhanced efficiency and efficacy having lesser side effects have been discussed.


Nanobiotechnology Nanoparticles Drug delivery Anticancer Antibacterial Antifungal Biosensor 



KPB gratefully acknowledges the director of CSIR-CSMCRI, Bhavnagar, for his constant support and encouragement.


  1. Ahmad A, Senapati S, Khan MI, Kumar R, Ramani R, Srinivas V et al (2003) Intracellular synthesis of gold nanoparticles by a novel alkalotolerant actinomycete, Rhodococcus species. Nanotechnology 14:824–828CrossRefGoogle Scholar
  2. Alani F, Moo-Young M, Anderson W (2012) Biosynthesis of silver nanoparticles by a new strain of Streptomyces sp. compared with Aspergillus fumigatus. World J Microbiol Biotechnol 28:1081–1086CrossRefPubMedGoogle Scholar
  3. Alghuthaymi MA, Almoammar H, Rai M, Said-Galiev E, Abd-Elsalam KA (2015) Myconanoparticles: synthesis and their role in phytopathogens management. Biotechnol Biotechnol Equip 29:221–236CrossRefPubMedPubMedCentralGoogle Scholar
  4. Aliosmanoglu A, Basaran I (2012) Nanotechnology in cancer treatment. J Nanomed Biotherapeut Discov 2:107. Scholar
  5. Aziz N, Pandey R, Barman I, Prasad R (2016) Leveraging the attributes of Mucor hiemalis-derived silver nanoparticles for a synergistic broad-spectrum antimicrobial platform. Front Microbiol 7:1984. Scholar
  6. Baharara J, Namvar F, Ramezani T, Hosseini N, Mohamad R (2014) Green synthesis of silver nanoparticles using Achillea biebersteinii flower extract and its anti-angiogenic properties in the rat aortic ring model. Molecules 19:4624–4634CrossRefPubMedGoogle Scholar
  7. Bao H, Hao N, Yang Y, Zhao D (2010a) Biosynthesis of biocompatible cadmium telluride quantum dots using yeast cells. Nano Res 3:481–489CrossRefGoogle Scholar
  8. Bao H, Lu Z, Cui X, Qiao Y, Guo J, Anderson JM, Li CM (2010b) Extracellular microbial synthesis of biocompatible CdTe quantum dots. Acta Biomater 6:3534–3541CrossRefPubMedGoogle Scholar
  9. Bhat R, Deshpande R, Sharanabasava VG, Do SH, Venkataraman A (2011) Photo-irradiated biosynthesis of silver nanoparticles using edible mushroom Pleurotus florida and their antibacterial activity studies. Bioinorg Chem Appl 7:650979Google Scholar
  10. Birla SS, Tiwari VV, Gade AK, Ingle AP, Yadav AP, Rai MK (2009) Fabrication of silver nanoparticles by Phoma glomerata and its combined effect against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Lett Appl Microbiol 48:173–179CrossRefPubMedGoogle Scholar
  11. Boroumand Moghaddam A, Namvar F, Moniri M, Md Tahir P, Azizi S, Mohamad R (2015) Nanoparticles biosynthesized by fungi and yeast: a review of their preparation, properties, and medical applications. Molecules 20:16540–16565CrossRefPubMedGoogle Scholar
  12. Bouwmeester H, Dekkers S, Noordam MY, Hagens WI, Bulder AS, Cde H et al (2009) Review of health safety aspects of nanotechnologies in food production. Regul Toxicol Pharmacol 53:52–62CrossRefPubMedGoogle Scholar
  13. Castro-Longoria E, Vilchis-Nestor AR, Avalos-Borja M (2011) Biosynthesis of silver, gold and bimetallic nanoparticles using the filamentous fungus Neurospora crassa. Colloids Surf B: Biointerfaces 83:42–48CrossRefPubMedGoogle Scholar
  14. Chuhan A, Zubair S, Tufail S, Sherwani A, Sajid M, Raman SC et al (2011) Fungus-mediated biological synthesis of gold nanoparticles: potential in detection of liver cancer. Int J Nanomedicine 6:2305–2519Google Scholar
  15. Dar J, Soytong K (2014) Construction and characterization of copolymer nanomaterials loaded with bioactive compounds from Chaetomium species. J Agr Technol 10:823–831Google Scholar
  16. Das SK, Das AR, Guha AK (2009) Gold nanoparticles: microbial synthesis and application in water hygiene management. Langmuir 25:8192–8199CrossRefPubMedGoogle Scholar
  17. Dastjerdi R, Montazer M (2010) A review on the application of inorganic nano-structured materials in the modification of textiles: focus on anti-microbial properties. Colloids Surf B: Biointerfaces 79:5–18CrossRefPubMedGoogle Scholar
  18. De Rosa G, Caraglia M (2013) New therapeutic opportunities from old drugs: the role of nanotechnology. J Bioequiv Availab 5:e30Google Scholar
  19. Duran N, Marcato PD, Alves OL, de Souza GIH, Esposito E (2005) Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains. J Nanobiotechnol 3:1–7CrossRefGoogle Scholar
  20. Durán N, Marcato PD, de Souza GIH, Alves OL, Esposito E (2007) Antibacterial effect of silver nanoparticles produced by fungal process on textile fabrics and their effluent treatment. J Biomed Nanotechnol 3:203–208CrossRefGoogle Scholar
  21. Eleftheriadou M, Pyrgiotakis G, Demokritou P (2016) Nanotechnology to the rescue: using nano-enabled approaches in microbiological food safety and quality. Curr Opin Biotechnol 44:87–93CrossRefPubMedPubMedCentralGoogle Scholar
  22. Ellis-Behnke RG, Liang YX, You SW, Tay DK, Zhang S, So KF, Schneide GE (2006) Nano neuro knitting: peptide nanofibers scaffold for brain repair and axon regeneration with functional return of vision. Proc Natl Acad Sci USA 103:5054–5059CrossRefPubMedGoogle Scholar
  23. El-Newehy MH, Al-Deyab SS, Kenawy E, Abdel-Megeed A (2012) Fabrication of electrospun antimicrobial nanofibers containing metronidazole using nanospider technology. Fiber Polym 13:709–717CrossRefGoogle Scholar
  24. Fadeel B, Garcia-Bennett AE (2010) Better safe than sorry: understanding the toxicological properties of inorganic nanoparticles manufactured for biomedical applications. Adv Drug Deliv Rev 62:362–374CrossRefPubMedGoogle Scholar
  25. Faraza M, Abbasia A, Naqvia FK, Khare N, Prasad R, Barman I, Pandey R (2018) Polyindole/CdS nanocomposite based turn-on, multi-ion fluorescence sensor for detection of Cr3+, Fe3+ and Sn2+ ions. Sensors & Actuators: B 269:195–202CrossRefGoogle Scholar
  26. Fateixa S, Neves MC, Almeida A, Oliveira J, Trindade T (2009) Anti-fungal activity of SiO2/Ag2S nanocomposites against Aspergillus niger. Colloids Surf B 74:304–308CrossRefGoogle Scholar
  27. Fayaz AM, Balaji K, Girilal M, Yadav R, Kalaichelvan PT, Venketesan R (2010) Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria. Nanomed Nanotechnol Biol Med 6:103–109CrossRefGoogle Scholar
  28. Formoso P, Muzzalupo R, Tavano L, De Filpo G, Nicoletta FP (2016) Nanotechnology for the environment and medicine. Mini-Rev Med Chem 16:668–675CrossRefPubMedGoogle Scholar
  29. Gajbhiye M, Kesharwani J, Ingle A, Gade A, Rai M (2009) Fungus mediated synthesis of silver nanoparticles and their activity against pathogenic fungi in combination with fluconazole. Nanomedicine 5:382–386CrossRefPubMedGoogle Scholar
  30. Ghosh P, Han G, De M, Kim CK, Rotello VM (2008) Gold nanoparticles in delivery applications. Adv Drug Deliv Rev 60:1307–1315CrossRefPubMedGoogle Scholar
  31. Giljohann DA, Seferos DS, Daniel WL, Massich MD, Patel PC, Mirkin CA (2010) Gold nanoparticles for biology and medicine. Angew Chem Int Ed 49:3280–3294CrossRefGoogle Scholar
  32. Gou M (2013) Promising application of nanotechnology in anticancer drug delivery. Drug Des 2:e117CrossRefGoogle Scholar
  33. Gupta S, Sharma K, Sharma R (2012) Myconanotechnology and application of nanoparticles in biology. Recent Res Sci Technol 4:36–38Google Scholar
  34. Gurunathan S, Lee KJ, Kalishwaralal K, Sheikpranb abu S, Vaidyanathan R, Eom SH (2009) Antiangiogenic properties of silver nanoparticles. Biomaterials 30:6341–6350CrossRefPubMedGoogle Scholar
  35. Gurunathan S, Raman J, Abd Malek SN, John PA, Vikineswary S (2013) Green synthesis of silver nanoparticles using Ganoderma neo-japonicum Imazeki: a potential cytotoxic agent against breast cancer cells. Int J Nanomedicine 8:4399–4413PubMedPubMedCentralGoogle Scholar
  36. Gurunathan S, Han J, Park JH, Kim YK (2014) A green chemistry approach for synthesizing biocompatible gold nanoparticles. Nanoscale Res Lett 9:1–11CrossRefGoogle Scholar
  37. Hafeli UO, Riffle JS, Harris-Shekhawat L, Carmichael-Baranauskas A, Mark F, Dailey JP (2009) Cell uptake and in vitro toxicity of magnetic nanoparticles suitable for drug delivery. Mol Pharm 6:1417–1428CrossRefPubMedGoogle Scholar
  38. Hsin YH, Chen CF, Huang S, Shih TS, Lai PS, Chueh PJ (2008) The apoptotic effect of nanosilver is mediated by a ROS and JNK-dependent mechanism involving the mitochondrial pathway in NIH3T3 cells. Toxicol Lett 179:130–139CrossRefPubMedGoogle Scholar
  39. Ingle A, Gade A, Pierrat S, Sonnichsen C, Rai M (2008) Mycosynthesis of silver nanoparticles using the fungus Fusarium acuminatum and its activity against some human pathogenic bacteria. Curr Nanosci 4:141–144CrossRefGoogle Scholar
  40. Iskandar F (2009) Nanoparticle processing for optical applications–a review. Adv Powder Technol 20:283–292CrossRefGoogle Scholar
  41. Jeyaraj M, Sathishkumar G, Sivanandhan G, MubarakAli D, Rajesh M, Arun R et al (2013) Biogenic silver nanoparticles for cancer treatment: an experimental report. Colloids Surf B: Biointerfaces 106:86–92CrossRefPubMedGoogle Scholar
  42. Jo YK, Kim BH, Jung G (2009) Antifungal activity of silver ions and nanoparticles on phytopathogenic fungi. Plant Dis 93:1037–1043CrossRefGoogle Scholar
  43. Joshi P, Bonde S, Gaikwad S, Gade A, Abd-Elsalam KA, Rai M (2013) Comparative studies on synthesis of silver nanoparticles by Fusarium oxysporum and Macrophomina phaseolina and its efficacy against bacteria and Malassezia furfur. J Bionanosci 7:1–5CrossRefGoogle Scholar
  44. Khosravi A, Shojaosadati SA (2009) Evaluation of silver nanoparticles produced by fungus Fusarium oxysporum. Int J Nanotechnol 6:973–983CrossRefGoogle Scholar
  45. Kumar R, Liu D, Zhang L (2008a) Advances in proteinous biomaterials. J Biobaased Mater Bioenergy 2:1–24CrossRefGoogle Scholar
  46. Kumar SA, Peter YA, Nadeau JL (2008b) Facile biosynthesis, separation and conjugation of gold nanoparticles to doxorubicin. Nanotechnology 19:495101CrossRefPubMedGoogle Scholar
  47. Li G, He D, Qian Y, Guan B, Gao S, Cui Y, Yokoyama K, Wang L (2012) Fungus-mediated green synthesis of silver nano- particles using Aspergillus terreus. Int J Mol Sci 13:466–476CrossRefPubMedGoogle Scholar
  48. Maite L, Carlesso N, Tung CH, Tang XW, Cory D, Scadden DT, Weissleder R (2000) Tat peptide derivatized magnetic nanoparticles allow in vivo tracking and recovery of progenitor cells. Nat Biotechnol 18:410–414CrossRefGoogle Scholar
  49. Mi Y, Shao Z, Vang J, Kaidar-Person O, Wang AZ (2016) Application of nanotechnology to cancer radiotherapy. Cancer Nanotechnol 7:11CrossRefPubMedPubMedCentralGoogle Scholar
  50. Mirunalini S, Arulmozhi V, Deeppalakshmi K, Krishnaveni M (2012) Intracellular biosynthesis and antibacterial activity of silver nanoparticles using edible mushrooms. Notulae Scientia Biologicae 4:55–61CrossRefGoogle Scholar
  51. Mishra A, Tripathy S, Wahab R, Jeong SH, Hwang I, Yang YB et al (2011) Microbial synthesis of gold nanoparticles using the fungus Penicillium brevicompactum and their cytotoxic effects against mouse mayo blast cancer C2C12 cells. Appl Microbiol Biotechnol 92:617–630CrossRefPubMedGoogle Scholar
  52. Musarrat J, Dwivedi S, Singh BR, Al-Khedhairy AA, Azam A, Naqvi A (2010) Production of antimicrobial silver nanoparticles in water extracts of the fungus Amylomyces rouxii strain KSU-09. Bioresour Technol 101:8772–8776CrossRefPubMedGoogle Scholar
  53. Nayak RR, Pradhan N, Behera D, Pradhan KM, Mishra S, Sukla LB, Mishra BK (2010) Green synthesis of silver nanoparticle by Penicillium purpurogenum NPMF, the process and optimization. J Nanopart Res 13:3129–3137CrossRefGoogle Scholar
  54. Nishida N, Yano H, Nishida T, Kamura T, Kojiro M (2006) Angiogenesis in cancer. Vasc Health Risk Manag 2:213–219CrossRefPubMedPubMedCentralGoogle Scholar
  55. Nithya R, Ragunathan R (2009) Synthesis of silver nanoparticle using Pleurotus sajor caju and its antimicrobial study. Digest J Nanomater Biostruct 4:623–629Google Scholar
  56. Nithya R, Ragunathan R (2014) In vitro synthesis, characterization and medical application of silver nanoparticle by using a lower fungi. Middle-East J Sci Res 21:922–928Google Scholar
  57. Oh SD, Lee S, Choi SH, Lee IS, Lee YM, Chun JH, Park HJ (2006) Synthesis of Ag and AgSiO2 nanoparticles by y-irradiation and their antibacterial and antifungal efficiency against Salmonella enterica serovar typhimurium and Botrytis cinerea. Colloids Surf A 275:228–233CrossRefGoogle Scholar
  58. Owaid MN, Raman J, Lakshmanan H, Al-Saeedi SS, Sabaratnam V, Abed IA (2015) Mycosynthesis of silver nanoparticles by Pleurotus cornucopiae var. citrinopileatus and its inhibitory effects against Candida sp. Mater Lett 153:186–190CrossRefGoogle Scholar
  59. Panchangam RBS, Dutta T (2015) Engineered nanoparticles for the delivery of anticancer therapeutics. J Pharm Drug Deliv Res 4:1CrossRefGoogle Scholar
  60. Pantidos N, Horsfall LE (2014) Biological synthesis of metallic nanoparticles by bacteria, fungi and plants. J Nanomed Nanotechnol 5:1–10CrossRefGoogle Scholar
  61. Park EJ, Yi J, Chung KH, Ryu DY, Choi J, Park K (2008) Oxidative stress and apoptosis induced by titanium dioxide nanoparticles in cultured BEAS-2B cells. Toxicol Lett 180:222–229CrossRefPubMedGoogle Scholar
  62. Podgaetsky VM, Tereshchenko SA, Reznichenko AV, Selishchev SV (2004) Laser-limiting materials for medical use. In: Optical technologies for industrial, environmental, and biological sensing; International Society for Optics and Photonics, Bellingham, pp 183–189Google Scholar
  63. Poulose S, Panda T, Nair PP, Theodore T (2014) Biosynthesis of silver nanoparticles. J Nanosci Nanotechnol 14:2038–2049CrossRefPubMedGoogle Scholar
  64. Prasad R (2016) Advances and applications through fungal nanobiotechnology. Springer, Cham. isbn:978-3-319-42989-2Google Scholar
  65. Prasad R (2017) Fungal nanotechnology: applications in agriculture, industry, and medicine. Springer International Publishing (ISBN 978-3-319-68423-9)Google Scholar
  66. Prasad R, Kumar V, Prasad KS (2014) Nanotechnology in sustainable agriculture: present concerns and future aspects. Afr J Biotechnol 13:705–713CrossRefGoogle Scholar
  67. Prasad R, Pandey R, Barman I (2016) Engineering tailored nanoparticles with microbes: quo vadis? WIREs Nanomed Nanobiotechnol 8:316–330. Scholar
  68. Prasad R, Bhattacharyya A, Nguyen QD (2017) Nanotechnology in sustainable agriculture: recent developments, challenges, and perspectives. Front Microbiol 8:1014. Scholar
  69. Rajakumar G, Rahuman A, Roopan SM, Khanna VG, Elango G, Kamaraj C et al (2012) Fungus-mediated biosynthesis and characterization of TiO2 nanoparticles and their activity against pathogenic bacteria. Spectrochim Acta A Mol Biomol Spectrosc 91:23–29CrossRefPubMedGoogle Scholar
  70. Raman JG, Reddy R, Lakshmanan H, Selvaraj H, Gajendran B, Nanjian R, Chinnasamy A, Sabaratnam V (2015) Mycosynthesis and characterization of silver nanoparticles from Pleurotus djamor var. roseus and their in vitro cytotoxicity effect on PC3 cells. Process Biochem 50:140–147CrossRefGoogle Scholar
  71. Riechelmann R, Grothey A (2017) Antiangiogenic therapy for refractory colorectal cancer: current options and future strategies. Ther Adv Med Oncol 9:106–126CrossRefPubMedGoogle Scholar
  72. Rosi NL, Mirkin CA (2005) Nanostructures in biodiagnostics. Chem Rev 105:1547–1562CrossRefPubMedGoogle Scholar
  73. Rudramurthy GR, Swamy MK, Sinniah UR, Ghasemzadeh A (2016) Nanoparticles: alternatives against drug-resistant pathogenic microbes. Molecules 21:836CrossRefGoogle Scholar
  74. Ruffolo SA, La Russa MF, Malagodi M, Oliviero Rossi C, Palermo AM, Crisci GM (2010) ZnO and ZnTiO3 nanopowders for antimicrobial stone coating. Appl Phys A Mater Sci Process 100:829–834CrossRefGoogle Scholar
  75. Saglam N, Yesilada O, Cabuk A, Sam M, Saglam S, Ilk S, Emul E, Celik PA, Gurel E (2016) Innovation of strategies and challenges for fungal nanobiotechnology. In: Prasad R (ed) Advances and applications through fungal nanobiotechnology. Springer, Cham, pp 25–46CrossRefGoogle Scholar
  76. Sardi JC, Scorzoni L, Bernardi T, Fusco-Almeida AM, Mendes Giannini MJ (2013) Candida species: current epidemiology, pathogenicity, biofilm formation, natural antifungal products and new therapeutic options. J Med Microbiol 62:10–24CrossRefPubMedGoogle Scholar
  77. Sarkar R, Kumbhakar P, Mitra AK (2010) Green synthesis of silver nanoparticles and its optical properties. Dig J Nanomater Biostruct 5:491–496Google Scholar
  78. Sastry M, Ahmad A, Islam Khan M, Kumar R (2003) Biosynthesis of metal nanoparticles using fungi and actinomycete. Curr Sci 85:162–170Google Scholar
  79. Scheinberg DA, Grimm J, Heller DA, Stater EP, Bradbury M, McDevitt MR (2017) Advances in the clinical translation of nanotechnology. Curr Opin Biotechnol 46:66–73CrossRefPubMedPubMedCentralGoogle Scholar
  80. Shahi SK, Patra M (2003) Biotechnological aspect for the synthesis of bioactive nanoparticle and their formulation active against human pathogenic fungi. Rev Adv Mat Sc 5:501–509Google Scholar
  81. Sheikpranbabu S, Kalishwaralal K, Venkataraman D, Eom SH, Park J, Gurunathan S (2009) Silver nanoparticles inhibit VEGF-and IL-1-beta-induced vascular permeability via Src dependent pathway in porcine retinal endothelial cells. J Nanobiotechnol 7:8CrossRefGoogle Scholar
  82. Singh D, Rathod V, Ninganagouda S, Hiremath J, Singh AK, Mathew J (2014) Optimization and characterization of silver nanoparticle by endophytic fungi Penicillium sp. isolated from Curcuma longa (turmeric) and application studies against MDR E.coli and S. aureus. Bioinorg Chem Appl 408021(2014):1–8Google Scholar
  83. Smith RR, Lodder RA (2013) When does a nanotechnology device become a drug? Size versus smarts. J Dev Drugs 2:e121CrossRefGoogle Scholar
  84. Taniguchi N (1974) On the basic concept of ‘nano-technology’. In: Proceedings of the international conference on production engineering Tokyo, Japan Soc Precision Engineering. Part II: 18–23Google Scholar
  85. Tiwari M (2012) Nano cancer therapy strategies. J Cancer Res Ther 8:19–22CrossRefPubMedGoogle Scholar
  86. Toffoli G, Rizzolio F (2013) Role of nanotechnology in cancer diagnostics. J Carcinogene Mutagene 4:e135CrossRefGoogle Scholar
  87. Vamanu CI, Cimpan MR, Hol PJ, Sornes S, Lie SA, Gjerdet NR (2008) Induction of cell death by TiO2 nanoparticles: studies on a human monoblastoid cell line. Toxicol in Vitro 22:1689–1696CrossRefPubMedGoogle Scholar
  88. Verma VC, Kharwar RN, Gange AC (2010) Biosynthesis of antimicrobial silver nanoparticles by the endophytic fungus Aspergillus clavatus. Nanomedicine 5:33–40CrossRefPubMedGoogle Scholar
  89. Will SEA, Favaron PO, Pavez MA, Florentino LC, Soares D, Oliveira FC, Rici REG, Miglino MA, Alcantara D, Mamizuka EM (2011) Bactericidal silver nanoparticles present an antiangiogenic effect in the chorioallantoic membrane model (CAM). Sci Against Microb Pathog Commun Curr Res Technol Adv 1:219–227Google Scholar
  90. Yehia RS, Al-Sheikh H (2014) Biosynthesis and characterization of silver nanoparticles produced by Pleurotus ostreatus and their anticandidal and anticancer activities. World J Microbiol Biotechnol 30:2797–2803CrossRefPubMedGoogle Scholar
  91. You C, Han C, Wang X, Zheng Y, Li Q, Hu X, Sun H (2012) The progress of silver nanoparticles in the antibacterial mechanism, clinical application and cytotoxicity. Mol Biol Rep 39:9193–9201CrossRefPubMedGoogle Scholar
  92. Zhao J, Bowman L, Zhang X, Vallyathan V, Young SH, Castranova V, Ding M (2009) Titanium dioxide (TiO2) nanoparticles induce JB6 cell apoptosis through activation of the caspase-8/Bid and mitochondrial pathways. J Toxicol Environ Heal A 72:1141–1149CrossRefGoogle Scholar
  93. Zheng B, Qian L, Yuan H, Xiao D, Yang X, Paau MC, Choi MMF (2010) Preparation of gold nanoparticles on eggshell membrane and their biosensing application. Talanta 82:177–183CrossRefPubMedGoogle Scholar
  94. Zhu T, Cloutier SG, Ivanov I, Knappenberger KL, Robel I, Zhang F (2012) Nanocrystals for electronic and optoelectronic applications. J Nanomater 2012:1–2. Scholar

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© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Kanti Bhooshan Pandey
    • 1
  • Brahm Kumar Tiwari
    • 2
  1. 1.CSIR-Central Salt & Marine Chemicals Research InstituteBhavnagarIndia
  2. 2.I.T.S Paramedical CollegeGhaziabadIndia

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