Special Stains for the Carbohydrate, Protein, Lipid, Nucleic Acid and Pigments
The various special stains are now the essential parts in routine laboratory works. The indications of special stains include demonstration of the various cellular products for diagnosis, identification of microbial organisms and also estimation of DNA and RNA content of the cell. The present chapter discusses on the various cellular products such as simple carbohydrate, mucin and fats. The principle and the steps of techniques to demonstrate those products are described in detail. The various organic and inorganic pigments are present in our body. The pigments may be endogenous or exogenous in origin. The chapter presents the staining techniques of different pigments on the histology tissue section.
KeywordsSpecial stain Carbohydrate Mucin Lipid Nucleic acid DNA Pigments PAS Alcian blue Mucicarmine Methyl green-pyronin Perls’ reaction Fouchet’s stain Grimelius stain
- 3.Lillie RD, Ashburn LL. Supersaturated solutions of fat stains in dilute isopropanol for demonstration of acute fatty degeneration not shown by Herxheimer’s technique. Arch Pathol. 1943;36:432–40.Google Scholar
- 4.Sheehan DC, Hrapchak BB. Theory and practice of histotechnology. 2nd ed. St. Louis: Mosby; 1980. p. 204–5.Google Scholar
- 7.Unna PG. Eine Modifikation der Pappenheimschen Farbung auf Granoplasma. Monatshefte für Praktische Dermatologie. 1902;35:76.Google Scholar
- 10.Lillie RD. Histopathologic technique and practical histochemistry. 2nd ed. New York: Blakiston; 1954.Google Scholar
- 11.von Kossa J. Ueber die im Organismus kunstlich erzeugbaren Verkalkungen. Beit Path Anat Allg Pathol. 1901;29:163.Google Scholar