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Sanger Sequencing and Next-Generation Gene Sequencing: Basic Principles and Applications in Pathology

  • Pranab Dey
Chapter

Abstract

DNA sequencing indicates the determination of the order of sequence of the base pair in DNA. Sanger sequencing is a widely used commercial DNA sequencing. The chapter elucidates the basic principle and steps of Sanger sequencing. Next-generation sequencing (NGS) is the newer gene sequencing technology that rapidly performs sequencing from abundant small fragments of DNA. The different types of NGS include pyrosequencing, microelectrophoretic methods, hybridization sequencing and real-time observation of single molecules. The basic principles, advantages and limitation of these techniques have been discussed in this chapter.

Keywords

DNA sequencing Sanger sequencing Pyrosequencing Microelectrophoretic methods Hybridization sequencing Real-time observation of single molecules 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Pranab Dey
    • 1
  1. 1.Education and Research (PGIMER)Post Graduate Institute of Medical Education and Research (PGIMER)ChandigarhIndia

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