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Processing of Tissue in Histopathology Laboratory

  • Pranab Dey
Chapter

Abstract

Processing of tissue is an important step because poorly processed tissue badly affects the section cutting and staining. The basic aim of processing is to remove water from the tissue section and to impregnate the tissue with another medium that can give support to the tissue. The various factors influence the tissue processing such as size of the tissue, temperature, viscosity, and negative pressure. Tissues are processed mainly by automated tissue processors which are of two types: tissue transfer processor and fluid transfer processor. Manual tissue processor is now obscured. It is used only in small laboratory and in emergency. Microwave processing accelerates the rate of processing. The present chapter covers all these aspects of tissue processing in histopathology.

Keywords

Tissue processing Tissue processor Microwave processing Paraffin wax Xylene Toluene Clearing agents Dehydrating agents 

References

  1. 1.
    Brain EB. Infiltration histological specimens with paraffin wax under vacuum. Basic factors and a new approach. Br Dent J. 1970;128(2):71–8.CrossRefPubMedGoogle Scholar
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    Leong AS, Leong FJ. Strategies for laboratory cost containment and for pathologist shortage: centralised pathology laboratories with microwave-stimulated histoprocessing and telepathology. Pathology. 2005;37(1):5–9.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Pranab Dey
    • 1
  1. 1.Education and Research (PGIMER)Post Graduate Institute of Medical Education and Research (PGIMER)ChandigarhIndia

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