Processing of Tissue in Histopathology Laboratory
Processing of tissue is an important step because poorly processed tissue badly affects the section cutting and staining. The basic aim of processing is to remove water from the tissue section and to impregnate the tissue with another medium that can give support to the tissue. The various factors influence the tissue processing such as size of the tissue, temperature, viscosity, and negative pressure. Tissues are processed mainly by automated tissue processors which are of two types: tissue transfer processor and fluid transfer processor. Manual tissue processor is now obscured. It is used only in small laboratory and in emergency. Microwave processing accelerates the rate of processing. The present chapter covers all these aspects of tissue processing in histopathology.
KeywordsTissue processing Tissue processor Microwave processing Paraffin wax Xylene Toluene Clearing agents Dehydrating agents