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Cytology Sample Procurement, Fixation and Processing

  • Pranab Dey
Chapter

Abstract

Proper sample collection, fixation and processing are the important components of routine laboratory technique in cytology. Unlike histopathology, the specimen collection is widely variable in different body samples in cytology. Different types of fixatives are used in various cytology samples. Moreover, the processing of the cytology is widely different in cytology samples. The chapter presents the different methods of sample collections in cytology with special emphasis on cervical cytology. Ethyl alcohol (95%) is the most commonly used fixative in cytology. The cytology samples are processed commonly by direct smear, centrifugation, cytocentrifugation, liquid-based preparation, Millipore technique and cell block. This chapter presents detailed description of these various techniques of processing the sample.

Keywords

Cytology sample collection Fixation Cytology processing Centrifugation Spray fixatives Cytocentrifugation Liquid-based preparation Millipore technique and cell block Buffy coat preparation 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Pranab Dey
    • 1
  1. 1.Education and Research (PGIMER)Post Graduate Institute of Medical Education and Research (PGIMER)ChandigarhIndia

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