Fixation of Histology Samples: Principles, Methods and Types of Fixatives

  • Pranab Dey


This chapter presents the different types of fixatives that are used in the histopathology and also cytopathology laboratories. The chemical constituents, mode of action, advantages and disadvantages of the fixatives are discussed. The various factors affect the rate of fixation such as pH, temperature, concentration, agitation, etc. This chapter discusses the different types of fixatives that are used for different tissues for different purposes. The various fixation artefacts are also discussed.


Fixative Mechanism of fixation Formaldehyde Microwave fixation Freeze-drying Ideal fixative Fixation artefact 


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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Pranab Dey
    • 1
  1. 1.Education and Research (PGIMER)Post Graduate Institute of Medical Education and Research (PGIMER)ChandigarhIndia

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