This chapter presents the different types of fixatives that are used in the histopathology and also cytopathology laboratories. The chemical constituents, mode of action, advantages and disadvantages of the fixatives are discussed. The various factors affect the rate of fixation such as pH, temperature, concentration, agitation, etc. This chapter discusses the different types of fixatives that are used for different tissues for different purposes. The various fixation artefacts are also discussed.
Bahr GF, Bloom G, Friberg U. Volume changes of tissues in physiological fluids during fixation in osmium tetroxide or formaldehyde and during subsequent treatment. Expl Cell Res. 1957;12:342–55.CrossRefGoogle Scholar
Jonsson N, Lagerst TS. The effect of formaldehyde containing fixatives on ribonuclease activity. Histoehemie. 1959;1:251–6.CrossRefGoogle Scholar
Kellenberger E, Johansen R, Maeder M, Bohrmann B, Stauffer E, Villiger W. Artefacts and morphological changes during chemical fixation. J Microsc. 1992;168(Pt 2):181–201.CrossRefPubMedGoogle Scholar