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Fixation of Histology Samples: Principles, Methods and Types of Fixatives

  • Pranab Dey
Chapter

Abstract

This chapter presents the different types of fixatives that are used in the histopathology and also cytopathology laboratories. The chemical constituents, mode of action, advantages and disadvantages of the fixatives are discussed. The various factors affect the rate of fixation such as pH, temperature, concentration, agitation, etc. This chapter discusses the different types of fixatives that are used for different tissues for different purposes. The various fixation artefacts are also discussed.

Keywords

Fixative Mechanism of fixation Formaldehyde Microwave fixation Freeze-drying Ideal fixative Fixation artefact 

References

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    Kellenberger E, Johansen R, Maeder M, Bohrmann B, Stauffer E, Villiger W. Artefacts and morphological changes during chemical fixation. J Microsc. 1992;168(Pt 2):181–201.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Pranab Dey
    • 1
  1. 1.Education and Research (PGIMER)Post Graduate Institute of Medical Education and Research (PGIMER)ChandigarhIndia

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