To Determine the Blood Glucose Levels by Folin and Wu Method

Abstract

The test is based on reducing property of glucose in hot alkaline solution. Proteins are precipitated by tungstic acid and removed by centrifugation. Protein-free filtrate of plasma contains glucose that reduces cupric ions of alkaline CuSO₄ to cuprous oxide, which in turn reduces phosphomolybdic acid to molybdenum blue, and color thus produced is measured colorimetrically at 430 nm.