Advertisement

Multiscale Simulation of Bioreactor Design and In Vitro Conditions

  • Adrien Baldit
  • Marzia Brunelli
  • Ana Campos Marin
  • Damien LacroixEmail author
Chapter
Part of the Frontiers of Biomechanics book series (FB, volume 3)

Abstract

Tissue grafts obtained from tissue engineering techniques can be developed with the application of cells in a scaffold within a bioreactor. In this chapter we present a multiscale method to simulate a bioreactor design that can adapt to the personalized tissue sought. It includes personalization of the bioreactor design but also personalization of the in vitro conditions. As the research area is going further and the computational possibilities as well, tools must be developed to design patient’s cell-specific pair of scaffold and bioreactor, as a virtual physiological human cell tool.

Thanks to a parametric geometry and a computational fluid dynamics model, we are able to design bioreactor chambers relying on the nearest boundary conditions in the bones to apply it to the bone substitute where cells have been seeded. First of all, considering an existing bioreactor chamber, we can design an optimized scaffold knowing the boundary conditions that the bioreactor chamber will impose. On the other hand, knowing the scaffold geometry used, a bioreactor chamber will be designed to reach appropriate environmental conditions at the cell scale.

It allowed testing two different bioreactor geometries showing no major interest within the simulation, but regarding the experimental process, the bubble traps presence is compulsory to avoid cell death. On the other hand, two scaffold geometries were tested highlighting a major difference regarding the local fluid flow within the scaffold pores and therefore on the cell development. Moreover, experimental analyses are required to correctly compare the simulation and improve the strength of the optimization process.

Keywords

Multiscale Tissue engineering Optimization process Bioreactor 

References

  1. Baldit A, Campos A, Brunelli M, Perrault C, Lacroix D (2014) Multi-scale modeling in tissue engineering: a virtual physiological approach. Proceeding virtual physiological human conferenceGoogle Scholar
  2. Barreto S, Clausen CH, Perrault CM, Fletcher DA, Lacroix D (2013) A multi-structural single cell model of force-induced interactions of cytoskeletal components. Biomaterials 26:6119–6126CrossRefGoogle Scholar
  3. Beebe DJ, Mensing GA, Walker GM (2002) Physics and applications of microfluidics in biology. Annu Rev Biomed Eng 4:261–286CrossRefGoogle Scholar
  4. Campos MA, Lacroix D (2015) The inter-sample structural variability of regular tissue-engineered scaffolds significantly affects the micromechanical local cell environment. Interface Focus 5(2):20140097–20140097CrossRefGoogle Scholar
  5. Chabiniok R, Wang VY, Hadjicharalambous M, Asner L, Lee J, Sermesant M, Kuhl E, Young AA, Moireau P, Nash MP, Chapelle D, Nordsletten DA (2016) Multiphysics and multiscale modelling, data–model fusion and integration of organ physiology in the clinic: ventricular cardiac mechanics. Interface Focus 6(2)CrossRefGoogle Scholar
  6. Geuzaine C, Remacle J-F (2009) Gmsh: a three-dimensional finite element mesh generator with built-in pre- and post-processing facilities. Int J Numer Methods Eng 79(11):1309–1331CrossRefGoogle Scholar
  7. Kausar H, Kishore RN (2013) Bone tissue engineering. Int J Pharm Pharm Sci 75:118Google Scholar
  8. Khayyeri H, Barreto S, Lacroix D (2015) Primary cilia mechanics affects cell mechanosensation: a computational study. J Theor Biol 379:38–46CrossRefGoogle Scholar
  9. Tanaka SM (1999) A new mechanical stimulator for cultured bone cells using piezoelectric actuator. J Biomech 32(4):427–430CrossRefGoogle Scholar
  10. Thorpe SD, Nagel T, Carroll SF, Kelly DJ (2013) Modulating gradients in regulatory signals within mesenchymal stem cell seeded hydrogels: a novel strategy to engineer zonal articular cartilage. PLoS One 8(4):e60764CrossRefGoogle Scholar
  11. Yu H-S, Kim J-J, Kim H-W, Lewis MP, Wall I (2016) Impact of mechanical stretch on the cell behaviors of bone and surrounding tissues. J Tissue Eng 7:1CrossRefGoogle Scholar
  12. Zhang Z-Y, Teoh SH, Teo EY, Chong MSK, Shin CW, Tien FT, Choolani M a, Chan JKY (2010) A comparison of bioreactors for culture of fetal mesenchymal stem cells for bone tissue engineering. Biomaterials 31(33):8684–8695CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Adrien Baldit
    • 1
  • Marzia Brunelli
    • 1
  • Ana Campos Marin
    • 1
  • Damien Lacroix
    • 1
    Email author
  1. 1.INSIGNEO Institute for in silico MedicineThe University of SheffieldSheffieldUK

Personalised recommendations