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Micropropagation and Conservation of Selected Endangered Anticancer Medicinal Plants from the Western Ghats of India

  • Mallappa Kumara Swamy
  • Sukanya Paramashivaiah
  • Lingayya Hiremath
  • Mohd Sayeed Akhtar
  • Uma Rani Sinniah
Chapter

Abstract

Globally, cancer is a constant battle which severely affects the human population. The major limitations of the anticancer drugs are the deleterious side effects on the quality of life. Plants play a vital role in curing many diseases with minimal or no side effects. Phytocompounds derived from various medicinal plants serve as the best source of drugs to treat cancer. The global demand for phytomedicines is mostly reached by the medicinal herbs from the tropical nations of the world even though many plant species are threatened with extinction. India is one of the mega diverse countries of the world due to its ecological habitats, latitudinal variation, and diverse climatic range. Western Ghats of India is one of the most important depositories of endemic herbs. It is found along the stretch of south western part of India and constitutes rain forest with more than 4000 diverse medicinal plant species. In recent times, many of these therapeutically valued herbs have become endangered and are being included under the red-listed plant category in this region. Due to a sharp rise in the demand for plant-based products, this rich collection is diminishing at an alarming rate that eventually triggered dangerous to biodiversity. Thus, conservation of the endangered medicinal plants has become a matter of importance. The conservation by using only in situ approaches may not be sufficient enough to safeguard such a huge bio-resource of endangered medicinal plants. Hence, the use of biotechnological methods would be vital to complement the ex vitro protection programs and help to reestablish endangered plant species. In this backdrop, the key tools of biotechnology that could assist plant conservation were developed in terms of in vitro regeneration, seed banking, DNA storage, pollen storage, germplasm storage, gene bank (field gene banking), tissue bank, and cryopreservation. In this chapter, an attempt has been made to critically review major endangered medicinal plants that possess anticancer compounds and their conservation aspects by integrating various biotechnological tools.

Keywords

Anticancer Conservation Endangered Micropropagation Seed bank 

References

  1. Aadhan K, Anand SP (2017) Survey of medicinal plants used for the treatment of diabetes by the Paliyar’s tribe in Sadhuragiri hills, Tamil Nadu, India. Int J Herbal Med 5:17–25Google Scholar
  2. Abdelfatah SA, Efferth T (2015) Cytotoxicity of the indole alkaloid reserpine from Rauwolfia serpentina against drug-resistant tumor cells. Phytomedicine 22:308–318PubMedCrossRefGoogle Scholar
  3. Amilineni U, Gangal V, Gudasalamani R, Karaba NN, Ramanan US (2016) Establishment and standardization of in vitro regeneration protocol in Nothapodytes nimmoniana Graham and evaluation of camptothecine (CPT) in tissue culture plants. Indian J Plant Physiol 21:1–7CrossRefGoogle Scholar
  4. Anilkumar ES, Mathew D, Nishanth Kumar S, Dileep Kumar BS, Latha PG (2014) A comparative study on in vitro antioxidant, anticancer and antimicrobial activity of the methanol extracts of the roots of four species of Aristolochia L. from southern Western Ghats of India. Int J Adv Res 2:153–164Google Scholar
  5. Ankad G, Upadhya V, Pai SR, Nimbalkar MS, Hegde HV, Joshi RK, Kholkute SD (2015) Evaluating Nothapodytes nimmoniana population from three localities of Western Ghats using camptothecin as phytochemical marker and selection of elites using a new-content range chart method. Pharmacogn Mag 11:90–95PubMedPubMedCentralCrossRefGoogle Scholar
  6. Anuradha M, Lokesh P, Pradeep SK, Balasubramanya S (2011) Improved propagation technique for Nothapodytes foetida. Int J Res Rev Pharm Appl Sci 1:270–277Google Scholar
  7. Aravind NA, Manjunath J, Rao D, Ganeshaiah KN, Shaanker RU, Vanaraj G (2005) Are red-listed species threatened. A comparative analysis of red-listed and non-red-listed plant species in the Western Ghats, India. Curr Sci 88:258–265Google Scholar
  8. Arumugam G, Swamy MK, Sinniah UR (2016) Plectranthus amboinicus (Lour.) Spreng: botanical, phytochemical, pharmacological and nutritional significance. Molecules 21:369PubMedPubMedCentralCrossRefGoogle Scholar
  9. Arya V, Shekhawat NS, Singh RP (2003) Micropropagation of Leptadenia reticulata-a medicinal plant. In Vitro Cell Dev Biol Plant 39:180–185CrossRefGoogle Scholar
  10. Ashokkumar K (2015) Gloriosa superba (L.): a brief review of its phytochemical properties and pharmacology. Int J Pharmacogn Phytochem Res 7:1190–1193Google Scholar
  11. Austin A, Herbals R (2008) A review on Indian sarsaparilla, Hemidesmus indicus (L.) R. Brzail. J Biol Sci 8:1–12CrossRefGoogle Scholar
  12. Baskar AA (2010) Cancer chemopreventive potential of luteolin-7-o-glucoside isolated from Ophiorrhiza mungos Linn. Nutr Cancer 63:130–138Google Scholar
  13. Chaturvedi M, Chaturvedi AK (2016) Acorus calamus: a divine drug for the human being. Imp J Interdisp Res 3:1499–1503Google Scholar
  14. Chauhan NS, Sharma V, Thakur M, Dixit VK (2010) Curculigo orchioides: the black gold with numerous health benefits. Zhong Xi Yi Jie He Xue Bao 8(7):613–623PubMedCrossRefGoogle Scholar
  15. Cherku PD, Devi BR, Reddy V, Manzelat SF (2016) Estimation of 2-hydroxy-4-methoxybenzaldehyde, lupeol and other unreported compounds in an elite ecotype of Hemidesmus indicus (L.) R. BR. Ann Phytomed 5:51–58Google Scholar
  16. Corlett RT, Primack RB (2008) Tropical rainforest conservation: a global perspective. In: Carson WP, Schnitzer SA (eds) Tropical forest community ecology. Blackwell Science, Oxford, pp 442–457Google Scholar
  17. Cruz-Cruz CA, Gonzalez-Arnao MT, Engelman F (2013) Biotechnology and conservation of plant biodiversity. Resources 2:73–95CrossRefGoogle Scholar
  18. Das SK, Rehman I, Ghosh A, Sengupta S, Majumdar P, Jana B, Das BB (2016) Poly (ADP-ribose) polymers regulate DNA topoisomerase I (Top1) nuclear dynamics and camptothecin sensitivity in living cells. Nucleic Acids Res 44:8363–8375PubMedPubMedCentralCrossRefGoogle Scholar
  19. Dey A, De JN (2011) Aristolochia indica L.: a review. Asian J Plant Sci 10:108–116CrossRefGoogle Scholar
  20. Ferlay J, Soerjomataram I, Dikshit R et al (2015) Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer 136:E359–E386CrossRefPubMedGoogle Scholar
  21. Francis SV, Senapati SK, Rout GR (2007) Rapid clonal propagation of Curculigo orchioides Gaertn, an endangered medicinal plant. In Vitro Cell Dev Biol Plant 43:140–143CrossRefGoogle Scholar
  22. Fulzele DP, Satdive RK, Pol BB (2001) Growth and production of camptothecin by cell suspension cultures of N. foetida. Planta Med 67:150–152PubMedPubMedCentralCrossRefGoogle Scholar
  23. Giridhar P, Gururaj HB, Ravishankar GA (2005) In vitro shoot multiplication through shoot tip cultures of Decalepis hamiltonii Wight and Arn., a threatened plant endemic to Southern India. In Vitro Cell Dev Biol Plant 41:77–80CrossRefGoogle Scholar
  24. Goel MK, Mehrotra S, Kukreja AK, Shanker K, Khanuja SP (2009) In vitro propagation of Rauwolfia serpentina using liquid medium, assessment of genetic fidelity of micropropagated plants, and simultaneous quantitation of reserpine, ajmaline, and ajmalicine. Methods Mol Biol 547:17–33PubMedCrossRefGoogle Scholar
  25. Greenwell M, Rahman PKSM (2015) Medicinal plants: their use in anticancer treatment. Int J Pharm Sci Res 6:4103–4112PubMedPubMedCentralGoogle Scholar
  26. Haghighi SR, Asadi MH, Akrami H, Baghizadeh A (2017) Anti-carcinogenic and anti-angiogenic properties of the extracts of Acorus calamus on gastric cancer cells. Avicenna J Phytomed 7:145–147Google Scholar
  27. Hariharan M, Sebastian DP, Benjamin S, Prashy P (2002) Somatic embryogenesis in Leptadenia reticulata Wight & Arn. A medicinal plant. Phytomorphology 52:155–160Google Scholar
  28. Harisaranraj R, Suresh K, Saravana Babu S (2010) Effect of growth regulators on micropropagation of Rauvolfia Serpentina (L.) Benth. Res J Biotechnol 5:51–55Google Scholar
  29. Jana S, Shekhawat GS (2011) Critical review on medicinally potent plant species: Gloriosa superba. Fitoterapia 82:293–301PubMedCrossRefGoogle Scholar
  30. Jayakumaran Nair A, Sudhakaran PR, Madhusudana Rao J, Ramakrishna SV (1992) Berberine synthesis by callus and cell suspension cultures of Coscinium fenestratum. Plant Cell Tissue Organ Cult 29:7–10CrossRefGoogle Scholar
  31. Jose B, Satheeshkumar K (2004) In vitro mass multiplication of Ophiorrhiza mungos Linn. Indian J Exp Biol 42:639–642PubMedGoogle Scholar
  32. Kainthla RP, Kashyap RS, Deopujari JY, Purohit HP, Taori GM, Daginawala HF (2006) Effect of Hemidesmus indicus (Anantmool) extract on IgG production and adenosine deaminase activity of human Lymphocytes in vitro. Indian J Pharm 38:190–193CrossRefGoogle Scholar
  33. Kaushik PS, Swamy MK, Balasubramanya S, Anuradha M (2015) Rapid plant regeneration, analysis of genetic fidelity and camptothecin content of micropropagated plants of Ophiorrhiza mungos Linn.-a potent anticancer plant. J Crop Sci Biotechnol 18:1–8CrossRefGoogle Scholar
  34. Khadke S, Kuvalekar A (2013) Direct somatic embryogenesis and plant regeneration from leaf and stem explants of N. foetida: a critically endangered plant species. Int J Plant Environ Sci 3:257–264Google Scholar
  35. Khan T, Krupadanam D, Anwar SY (2008) The role of phytohormone on the production of berberine in the calli cultures of an endangered medicinal plant, turmeric (Coscinium fenestratum I.) Afr J Biotechnol 7:3244–3246Google Scholar
  36. Krishnakumar G, Rameshkumar K, Srinivas P, Satheeshkumar K, Krishnan P (2012) Estimation of camptothecin and pharmacological evaluation of Ophiorrhiza prostrata D. Don and Ophiorrhiza mungos L. Asian Pac J Trop Biomed (2):S727–S731CrossRefGoogle Scholar
  37. Krishnan PN, Decruse SW, Radha RK (2011) Conservation of medicinal plants of Western Ghats, India and its sustainable utilization through in vitro technology. In Vitro Cell Dev Biol 47:110–122CrossRefGoogle Scholar
  38. Krishnan SA, Dileepkumar R, Nair AS, Oommen OV (2014) Studies on neutralizing effect of Ophiorrhiza mungos root extract against Daboia russelii venom. J Ethnopharmacol 151:543–547PubMedCrossRefGoogle Scholar
  39. Kumara SM, Sudipta KM, Lokesh P, Neeki A, Rashmi W, Bhaumik H, Darshil H, Vijay R, Kashyap SSN (2012) Phytochemical screening and in vitro antimicrobial activity of Bougainvillea spectabilis flower extracts. Int J Phytomed 4:375–379Google Scholar
  40. Kumari R, Rathi B, Rani A, Bhatnagar S (2013) Rauvolfia serpentina L. Benth. ex Kurz.: phytochemical, pharmacological and therapeutic aspects. Int J Pharm Sci Rev Res 23:348–355Google Scholar
  41. Kuo PC, Li YC, Wu TS (2012) Chemical constituents and pharmacology of the Aristolochia species. J Tradit Complement Med 2:249–266PubMedPubMedCentralCrossRefGoogle Scholar
  42. Lee JH, Han TH (2011) Micropropagation of the plantlets derived from seeds in the genus Acorus (A. calamus and A. gramineus). Hortic Environ Biotechnol 52:89.  https://doi.org/10.1007/s13580-011-0154-0 CrossRefGoogle Scholar
  43. Lloyd G, McCown B (1980) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia by use of shoot tip culture. Int Plant Prop Soc Proc 30:421–427Google Scholar
  44. Lobay D (2015) Rauwolfia in the treatment of hypertension. Integr Med 14:40–46Google Scholar
  45. Madhavan SC, Bose C, Mathew T, Perakathusseril AB (2014) Indian medicinal plant, Coscinium fenestratum-A new bio source for the multifunctional bio active molecule ecdysterone. Int J Herb Med 2:5–9Google Scholar
  46. Mariappan S (2012) Therapeutic efficiency of aristolochic acid on oral cancer induced experimental rats. J Pharm Biol Sci 4:12–20Google Scholar
  47. Martin KP (2004) Benzyladenine induced somatic embryogenesis and plant regeneration of Leptadenia reticulata. Biol Plant 48(2):285–288CrossRefGoogle Scholar
  48. Mazumder PM, Das S, Das MK (2010) Cytotoxic activity of methanolic extracts of Berberis aristata DC and Hemidesmus indicus R. Br. in MCF7 cell line. J Curr Pharm Res 1:12–15Google Scholar
  49. Meena AK, Rao MM, Singh A, Kumari S (2010) Phytochemical and preliminary phytochemical studies on the rhizome of Acorus calamus Linn. Int J Pharm Pharm Sci 2:130–131Google Scholar
  50. Mohanty SK, Mallappa KS, Godavarthi A, Subbanarasiman B, Maniyam A (2014) Evaluation of antioxidant, in vitro cytotoxicity of micropropagated and naturally grown plants of Leptadenia reticulata (Retz.) Wight & Arn.-an endangered medicinal plant. Asian Pac J Trop Med 7:S267–S271Google Scholar
  51. Mohanty SK, Swamy MK, Sinniah UR, Anuradha M (2017) Leptadenia reticulata (Retz.) Wight & Arn. (Jivanti): botanical, agronomical, phytochemical, pharmacological, and biotechnological aspects. Molecules 22:1019CrossRefGoogle Scholar
  52. Murali VP, Kuttan G (2015) Enhancement of cancer chemotherapeutic efficacy of cyclophosphamide by Curculigo orchioides Gaertn. and its ameliorative effects on cyclophosphamide-induced oxidative stress. Integr Cancer Ther 14:172–183PubMedCrossRefGoogle Scholar
  53. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  54. Nagdeva KP, Singh R (2012) Anticancer activity of leaves of Clerodendron serratum Spreng. Am J PharmTech Res 2(4):451–461Google Scholar
  55. Nagesh KS (2008) High frequency of multiple shoot induction of Curculigo orchioides Gaertn. shoot tip v/s rhizome disc. Taiwania 53:242–247Google Scholar
  56. Nagesh KS, Nayaka H, Shylaja M, Dharmesh S, Shanthamma C, Pullaih T (2008) In vitro propagation and antioxidant activity of Curculigo orchioides Gaertn. J Trop Med Plants 9:404–410Google Scholar
  57. Nagesh KS, Bhgya lakashmi N, Shanthamma C (2009) Role of polarity in de novo shoot bud initiation in rhizome disc of Curculigo orchioides Gaertn. and its encapsulation and storability. Acta Physiol Plant 31:699–704CrossRefGoogle Scholar
  58. Nagesh KS, Shanthamma C, Pullaiah T (2010) Somatic embryogenesis and plant regeneration from callus cultures of Curculigo orchioides Gaertn. Indian J Biotechnol 9:408–413Google Scholar
  59. Namdeo AG, Priya T, Bhosale BB (2012) Micropropagation and production of camptothecin form in vitro plants of Ophiorrhiza mungos. Asian Pacific J Trop Biomed 2:S662–S666CrossRefGoogle Scholar
  60. Pai SR, Nimbalkar MS, Pawar NV, Patil RP, Dixit GB (2010) Seasonal discrepancy in phenolic content and antioxidant properties from bark of Nothapodytes nimmoniana (Grah.) Mabb. Int J Pharm Biol Sci 1:1–17Google Scholar
  61. Pai SR, Pawar NV, Nimbalkar MS, Kshirsagar PR, Kolar FK, Dixit GB (2013) Seasonal variation in content of camptothecin from the bark of Nothapodytes nimmoniana (Grah.) Mabb., using HPLC analysis. Pharm Res 5:219–223Google Scholar
  62. Palani S, Raja S, Praveen Kumar R, Venkadesan D, Devi K, Sivaraj A, Senthilkumar B (2009) Therapeutic efficacy of antihepatotoxic and antioxidant activities of Acorus calamus on acetaminophen induced toxicity in rat. Int J Integr Biol 7:39–44Google Scholar
  63. Panda SK, Debajoyti D, Bichitra N, Tripathy LN (2012) Phyto-pharmacognostical studies and quantitative determination of reserpine in different parts of Rauwolfia (spp.) of eastern Odisha by UV spectroscopy method. Asian J Plant Sci Res 2:151–162Google Scholar
  64. Pantaziz P, Han Z, Chatterjee D, Wyche J (1999) Water-insoluble camptothecin analogues as potential antiviral drugs. J Biomed Sci 6:1–7CrossRefGoogle Scholar
  65. Parabia MF, Gami B, Kothari LI, Mohan JSS, Parabia HM (2007) Effect of plant growth regulators on in vitro morphogenesis of Leptadenia reticulata from nodal explants. Curr Sci 92:1290–1293Google Scholar
  66. Park SY, Cheng YC (2005) Poly (ADP-ribose) polymerase-1 could facilitate the religation of topoisomerase I-linked DNA inhibited by camptothecin. Cancer Res 65:3894–3902PubMedCrossRefGoogle Scholar
  67. Pattar PV, Jayaraj M (2012) In vitro regeneration of plantlets from leaf and nodal explants of Aristolochia indica L.-an important threatened medicinal plant. Asian Pac J Trop Biomed 2:S488–S493CrossRefGoogle Scholar
  68. Plummer M, de Martel C, Vignat J, Ferlay J, Bray F, Franceschi S (2016) Global burden of cancers attributable to infections in 2012: a synthetic analysis. Lancet Glob Health 4:e609–e616.  https://doi.org/10.1016/S2214-109X(16)30143-7 CrossRefPubMedGoogle Scholar
  69. Poornima BS, Hegde PL, Pradeep HA (2015) Pharmacological review on Clerodendrum serratum Linn. Moon. J Pharmacogn Phytochem 3:126–130Google Scholar
  70. Potikanond S, Chiranthanut N, Khonsung P, Teekachunhatean S (2015) Cytotoxic effect of Coscinium fenestratum on human head and neck cancer cell line (HN31). Evid Based Complement Alternat Med 2015:701939.  https://doi.org/10.1155/2015/701939 CrossRefPubMedPubMedCentralGoogle Scholar
  71. Prakash L, Middha SK, Mohanty SK, Swamy MK (2016) Micropropagation and validation of genetic and biochemical fidelity among regenerants of Nothapodytes nimmoniana (Graham) Mabb. Employing ISSR markers and HPLC. 3Biotech 6:171.  https://doi.org/10.1007/s13205-016-0490-y CrossRefGoogle Scholar
  72. Quraishi A, Mehar S, Sahu D, Jadhav SK (2017) In vitro mid-term conservation of Acorus calamus L. via cold storage of encapsulated microrhizome. Braz Arch Biol Technol 60:e17160378.  https://doi.org/10.1590/1678-4324-2017160378 CrossRefGoogle Scholar
  73. Radha RK, Decruse WS, Krishnan PN (2012) Plant cryopreservation. In: Katkov I (ed) Current frontiers in cryopreservation. InTech. http://www.intechopen.com/books/current-frontiers-in-cryopreservation/plant-cryopreservation. Assessed on 10.08.2017Google Scholar
  74. Rajasekharan PE, Abdul Kareem VK, Vasantha Kumar T (2010) Optimization of protocols for in vitro multiplication and conservation of Nothapodytes nimmoniana, an endangered medicinal plant. Acta Hortic 865:53–58CrossRefGoogle Scholar
  75. Ramasubbu R (2010) Protecting the wild beauties. Sci Rep 7:19–22Google Scholar
  76. Ramulu DR, Murthy KSR, Pullaiah T (2003) Regeneration of plants from root segments derived from aseptic seedlings of Hemidesmus indicus R. Br. Phytomorphology 53:293–298Google Scholar
  77. Rani AS, Subhadra VV, Reddy VD (2000) In vitro propagation of Acorus calamus Linn.-a medicinal plant. Indian J Exp Biol 38:730–732Google Scholar
  78. Ranjan A, Jain P, Singh B, Singh P, Sharma HP (2016) Acorus calamus L.: an insight review of botany, chemistry, medicinal uses and cultural practice. J Chem Biol Phys Sci 66:1027–1045Google Scholar
  79. Raveendran VV (2015) Camptothecin-discovery, clinical perspectives and biotechnology. Nat Prod Chem Res 3:175.  https://doi.org/10.4172/2329-6836.1000175 CrossRefGoogle Scholar
  80. Ray A, Bhattacharya S (2008) Cryopreservation of in vitro grown nodal segments of Rauvolfia serpentina by PVS2 vitrification. Cryo Letters 29:321–328Google Scholar
  81. Reddy MC, Murthy KSR (2013) A review on Decalepis hamiltonii Wight Arn. J Med Plants Res 7:3014–3029Google Scholar
  82. Rojsanga P, Sukhthankar M, Krisanapun C, Gritsanapan W, Buripakdi Lawson D, Baek SJ (2010) In vitro anti-proliferative activity of alcoholic stem extract of Coscinium fenestratum in human colorectal cancer cells. Exp Ther Med 1:181–186PubMedPubMedCentralCrossRefGoogle Scholar
  83. Samydurai P, Saradha M, Ramakrishnan R, Kumar SS, Thangapandian V (2016) Micropropagation prospective of cotyledonary explants of Decalepis hamiltonii Wight & Arn.-an endangered edible species. Indian J Biotechnol 15:256–260Google Scholar
  84. Sandhyarani N, Kishor R, Sharma GJ (2011) Clonal propagation of triploid Acorus calamus Linn. Using dual-phase culture system. J Crop Sci Biotech 14:213–217CrossRefGoogle Scholar
  85. Santny AS (2016) Review of anticancer activity of Gloriosa superba and Polyalthia longifolia against lung cancer. Asia Pac J Res 1:1–3Google Scholar
  86. Sathiyanarayanan L, Sinnathambi A, Chidambaranathan N (2007) Anticarcinogenic activity of Leptadenia reticulata against Daltons ascitic lymphoma. J Pharmacol Ther 6:133–135Google Scholar
  87. Sarvalingam A, Rajendran A (2016) Rare, endangered and threatened (RET) climbers of southern western Ghats, India. Rev Chil Hist Nat 89:9.  https://doi.org/10.1186/s40693-016-0058-6 CrossRefGoogle Scholar
  88. Sathyanarayana N, Rajesha R, Vikas PB, Kumar TN (2008) Somatic embryogenesis and plant regeneration from stem explants of Leptadenia reticulata (Retz.) Wight. & Arn. Indian J Biotechnol 7:250–254Google Scholar
  89. Sayeed Hassan AKM, Roy KS (2005) Micropropagation of Gloriosa superba L. through high frequency shoot proliferation. Plant Tissue Cult 15:67–74Google Scholar
  90. Schenk RV, Hilderbrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledons plant cultures. Can J Bot 50:199–204CrossRefGoogle Scholar
  91. Selvarasu A, Kandhasamy R (2012) In vitro tuberization of glory lily (Gloriosa superba L.) J Hortic Forest 4:81–84Google Scholar
  92. Senapati SK, Lahere N, Tiwary BN (2014) Improved in vitro clonal propagation of Rauwolfia serpentina L. Benth–an endangered medicinal plant. Plant Biosys 148:885–888CrossRefGoogle Scholar
  93. Senarath WTPSK (2010) In vitro propagation of Coscinium fenestratum (Gaertn.) Colebr. (Menispermaceae)-an endangered medicinal plant. J Nat Sci Found 38:219–223Google Scholar
  94. Shah SN, Husaini AM, Shirin F (2013) Micropropagation of the Indian birthwort Arsitolochia indica L. Int J Biotechnol Mol Biol Res 4:86–92CrossRefGoogle Scholar
  95. Sharma S, Shahzad A (2014) An overview on Decalepis: a genus of woody medicinal climbers. J Plant Sci Res 1(1):104Google Scholar
  96. Sharma S, Thokchom R (2014) A review on endangered medicinal plants of India and their conservation. J Crop Weed 10:205–218Google Scholar
  97. Sharma M, Rai SK, Purshottam DK, Jain M, Chakrabarty D, Awasthi A, Nair KN, Sharma AK (2009) In vitro clonal propagation of Clerodendrum serratum (Linn.) moon (barangi): a rare and threatened medicinal plant. Acta Physiol Plant 31:379–383CrossRefGoogle Scholar
  98. Shathish K, Guruvayoorappan C (2014) Decalepis hamiltonii inhibits tumor progression and metastasis by regulating the inflammatory mediators and nuclear factor κB subunits. Integr Cancer Ther 13(2):141–151PubMedCrossRefGoogle Scholar
  99. Shekhawat MS, Manokari M (2016) In vitro regeneration frequency, micro-morphological studies and ex vitro rooting of Hemidesmus indicus (L.) R. Br.: a multi-potent endangered climber. Indian J Plant Physiol 21:151–160CrossRefGoogle Scholar
  100. Shekhawat NS, Kackar A, Rathore MS, Singh M, Dagla HR, Arya V (2006) Establishment and economic evaluation of micropropagated Jeewanti Leptadenia reticulata (Wight & Arn.) plants in field. Nat Prod Rad 5:311–314Google Scholar
  101. Siddique NA, Bari MA, Pervin MM, Nahar N, Banu LA, Paul KK, Kabir MH, Huda AK, Ferdaus KM, Hossin MJ (2006) Plant regeneration from axillary shoots derived callus in Aristolochia indica Linn. An endangered medicinal plant in Bangladesh. Pak J Biol Sci 9:1320–1323CrossRefGoogle Scholar
  102. Siegel RL, Miller KD, Jemal A (2016) Cancer statistics, 2016. CA A Cancer J Clin 66:7–30CrossRefGoogle Scholar
  103. Singh J, Jain S, Khanuja S (2006) Curculigo Orchioides Gaertn. (Kali musali): an endangered medicinal plants of commercial value. Nat Prod Rad 5:369–372Google Scholar
  104. Singh R, Sharma PK, Malviya R (2011) Pharmacological properties and ayurvedic value of Indian buch plant (Acorus calamus): a short review. Adv Biol Res 5:145–154Google Scholar
  105. Sreekumar S, Seeni S, Pushpangadan P (2000) Micropropagation of Hemidesmus indicus for cultivation and production of 2-hydroxy 4-methoxy benzaldehyde. Plant Cell Tissue Organ Cult 62:211–218CrossRefGoogle Scholar
  106. Srivastava A, Rao LJM, Shivanandappa T (2007) Isolation of ellagic acid from the aqueous extract of the roots of Decalepis hamiltonii: antioxidant activity and cytoprotective effect. Food Chem 103:224–233CrossRefGoogle Scholar
  107. Subramaniyan V, Saravanan R, Baskaran D, Ramalalingam S (2015) In vitro free radical scavenging and anticancer potential of Aristolochia indica L. against MCF-7 cell line. Int J Pharm Pharm Sci 7:392–396Google Scholar
  108. Sudipta KM, Swamy MK, Balasubramanya S, Anuradha M (2011) Cost effective approach for in vitro propagation of (Leptadenia reticulata Wight & Arn.)-a threatened plant of medicinal importance. J Phytology 3:72–79Google Scholar
  109. Sudipta KM, Swamy MK, Anuradha M (2013) Influence of various carbon sources and organic additives on in vitro growth and morphogenesis of Leptadenia reticulata (Wight & Arn), a valuable medicinal plant of India. Int J Pharm Sci Rev Res 21:174–179Google Scholar
  110. Sudipta KM, Kumara Swamy M, Balasubramanya S, Anuradha M (2014) Assessment of genetic fidelity, antioxidant enzyme activity and proline content of micropropagated and field grown plants of Leptadenia reticulata (wight & arn.)-an endangered medicinal plant. Plant Cell Biotechnol Mol Biol 15:127–135Google Scholar
  111. Sundravelan R, Desireddy B, Ciddi V (2004) Production of camptothecin from callus culture of N. foetida (Wright) Sleumer. Indian J Biotechnol 3:452–453Google Scholar
  112. Suri SS, Arora DK, Ramawat KG (2009) A method for large-scale multiplication of Curculigo orchioides through bulbil formation from leaf explant in shake flask culture. Indian J Exp Biol 38:145–148Google Scholar
  113. Susila T, Reddy GS, Jyothsna D (2013) Standardization of protocol for in vitro propagation of an endangered medicinal plant Rauwolfia serpentina Benth. J Med Plant Res 7:2150–2153Google Scholar
  114. Swamy MK, Sinniah UR (2015) A comprehensive review on the phytochemical constituents and pharmacological activities of Pogostemon cablin Benth.: an aromatic medicinal plant of industrial importance. Molecules 20:8521–8547CrossRefPubMedGoogle Scholar
  115. Swamy MK, Sinniah UR (2016) Patchouli (Pogostemon cablin Benth.): Botany, agrotechnology and biotechnological aspects. Ind Crop Prod 87:161–176CrossRefGoogle Scholar
  116. Swamy MK, Sinniah UR, Akhtar MS (2015) In vitro pharmacological activities and GC-MS analysis of different solvent extracts of Lantana camara leaves collected from tropical region of Malaysia. Evid Based Complement Alternat Med 2015:506413.  https://doi.org/10.1155/2015/506413 CrossRefPubMedPubMedCentralGoogle Scholar
  117. Swamy MK, Sinniah UR, Akhtar MS (2016) Antimicrobial properties of plant essential oils against human pathogens and their mode of action: an updated review. Evid Based Complement Alternat Med 2016:3012462.  https://doi.org/10.1155/2016/3012462 CrossRefPubMedPubMedCentralGoogle Scholar
  118. Swamy MK, Arumugam G, Kaur R, Ghasemzadeh A, Yusoff MM, Sinniah UR (2017) GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves. Evid Based Complement Alternat Med 2017:1517683.  https://doi.org/10.1155/2017/1517683 CrossRefPubMedPubMedCentralGoogle Scholar
  119. TBGRI (2009–2010) Annual report. Tropical Botanic Garden and Research Institute, Thiruvananthapuram, IndiaGoogle Scholar
  120. Tejavathi DH, Raveesha HR, Shobha K (2011) Evaluation of different treatments to improve the seed germination among the populations of N. foetida (WT) Sleumer. Indian J Fundam Appl Life Sci 1:187–192Google Scholar
  121. Thangavel K, Ravichandran P, Ebbie MG, Manimekalai V (2014) In vitro microrhizome production in Decalepis hamiltonii. Afr J Biotechnol 13:1208–1313Google Scholar
  122. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A (2015) Global cancer statistics, 2012. CA Cancer J Clin 65:87–108PubMedPubMedCentralCrossRefGoogle Scholar
  123. Tungpradit R, Sinchaikul S, Phutrakul S, Wongkham W, Chen ST (2010) Anti-cancer compound screening and isolation: Coscinium fenestratum, Tinospora crispa and Tinospora cordifolia. Chiang Mai J Sci 37:476–488Google Scholar
  124. Tungpradit R, Sinchaikul S, Phutrakul S, Wongkham W, Chen S-T (2011) Antiproliferative activity of berberine from Coscinium fenestratum on NCI-H838 cell line. Chiang Mai J Sci 38:85–94Google Scholar
  125. Vedavathy S (2004) Decalepis hamiltonii Wight & Arn. – an endangered source of indigenous health drink. Nat Prod Radiance 3:22–23Google Scholar
  126. Venukumar MR, Latha MS (2002) Antioxidant activity of Curculigo orchioides in carbon tetrachloride induced hepatopathy in rats. Indian J Clin Biochem 17:80–87PubMedPubMedCentralCrossRefGoogle Scholar
  127. Verma S, Singh N (2012) In vitro mass multiplication of Acorus calamus L.-an endangered medicinal plant. Am Eur J Agric Environ Sci 12:1514–1521Google Scholar
  128. Verma KC, Verma SK (2010) Alkaloids analysis in root and leaf fractions of sarpaghanda (Rauwolfia serpentina). Agric Sci Dig 30:133–135Google Scholar
  129. Vidya SM, Krishna V, Manjunatha BK, Pradeepa MR (2012) Micropropagation of Clerodendrum serratum L. through direct and indirect organogenesis. Plant Tissue Cult Biotechnol 22:179–185Google Scholar
  130. Xia LF, Liang SH, Wen H, Tang J, Huang Y (2016) Anti-tumor effect of polysaccharides from rhizome of Curculigo orchioides Gaertn on cervical cancer. Trop J Pharm Res 15:1731–1737CrossRefGoogle Scholar
  131. Yadav K, Groach R, Aggarwal A, Singh N (2015) A reliable protocol for micropropagation of Gloriosa superba L. (Colchicaceae). Asian Pac J Mol Biol Biotechnol 23:242–251Google Scholar
  132. Yamazaki Y, Sudo H, Yamazaki M, Aimi N, Saito K (2003) Camptothecin biosynthetic genes in hairy roots of Ophiorrhiza pumila: cloning, characterization and differential expression in tissues and by stress compounds. Plant Cell Physiol 44:395–403PubMedPubMedCentralCrossRefGoogle Scholar
  133. Yin SY, Wei WC, Jian FY, Yang NS (2013) Therapeutic applications of herbal medicines for cancer patients. Evid Based Complement Alternat Med 2013:302426.  https://doi.org/10.1155/2013/302426 CrossRefPubMedPubMedCentralGoogle Scholar
  134. Yue Q, Gao G, Zou G, Yu H, Zheng X (2017) Natural products as adjunctive treatment for pancreatic cancer: recent trends and advancements. Biomed Res Int 2017:8412508.  https://doi.org/10.1155/2017/8412508 CrossRefPubMedPubMedCentralGoogle Scholar
  135. Zalke AS, Kulkarni AV, Shirode DS, Duraiswamy B (2010) In vivo anticancer activity of Clerodendrum serratum (L) moon. Res J Pharm Biol Chem Sci 1:89–98Google Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Mallappa Kumara Swamy
    • 1
    • 2
  • Sukanya Paramashivaiah
    • 3
  • Lingayya Hiremath
    • 3
  • Mohd Sayeed Akhtar
    • 4
  • Uma Rani Sinniah
    • 1
  1. 1.Department of Crop Science, Faculty of AgricultureUniversiti Putra MalaysiaSerdangMalaysia
  2. 2.Department of BiotechnologyPadmashree Institute of Management and SciencesBengaluruIndia
  3. 3.Department of BiotechnologyR.V. College of EngineeringBengaluruIndia
  4. 4.Department of BotanyGandhi Faiz-e-Aam CollegeShahjahanpurIndia

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