Use of TDZ for Micropropagation of Some Mediterranean Crop Species

  • Mouaad Amine Mazri
  • Ilham Belkoura
  • Reda Meziani


Plant tissue culture is now a widely used technology for many applications. Plant growth regulators (PGRs) play an important role in this technology. Auxins and cytokinins are by far the most commonly used PGR classes in plant tissue culture. N-Phenyl-N′-1,2,3-thiadiazol-5-ylurea, also known as thidiazuron or TDZ, is a synthetic PGR and a phenylurea derivative with a strong cytokinin-like activity. TDZ has been successfully used for the micropropagation of several plant species and sometimes was reported to be more effective than adenine-based cytokinins or to fulfil both the auxin and the cytokinin requirement for in vitro growth and differentiation. In this chapter, we will focus on the use of TDZ for the micropropagation of six Mediterranean crop species. Thus, we will report some findings from past and recent studies in which TDZ was employed for in vitro culture and regeneration of Olea europaea L., Citrus spp., Ceratonia siliqua L., Punica granatum L., Ficus carica L., and Prunus dulcis Mill. We will also highlight the specific action of TDZ depending on its concentration as well as the species, the genotype, the explant, and the concentration of the associated PGRs.


Almond Carob Citrus Fig Olive Pomegranate TDZ 


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© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Mouaad Amine Mazri
    • 1
  • Ilham Belkoura
    • 2
  • Reda Meziani
    • 3
  1. 1.Institut National de la Recherche Agronomique, CRRA-Marrakech, UR Agro-Biotechnologie, Laboratoire de Biotechnologie VégétaleMarrakechMorocco
  2. 2.Ecole Nationale d’Agriculture, Département des Sciences de Base, Laboratoire de Culture In VitroMeknesMorocco
  3. 3.Institut National de la Recherche Agronomique, CRRA-Errachidia, UR Systèmes Oasiens, Laboratoire National de Culture des Tissus du Palmier DattierErrachidiaMorocco

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