Perspectives in High-Throughput Phenotyping of Qualitative Traits at the Whole-Plant Level



Recent advances in technology have enabled the rapid development of high-throughput automated and semi-automated field and laboratory phenotyping platforms worldwide. In this review, we discuss possible ways of matching the qualitative traits of the above-ground parts of crop plants, also defining the target traits and possible approaches that would be useful in automated phenotyping systems. Optical tools based on light reflectance are presented as a high-throughput and low-cost alternative to some destructive analytical methods. Special attention is paid to hyperspectral imaging and its integration in high-throughput phenotyping systems, as well as its special applications for the assessment of specific plant material traits associated with food quality.


Phenotyping Phenomics Hyperspectral imaging Qualitative traits 



Desorption electrospray ionization mass spectrometry imaging


Deoxyribonucleic acid


Electrospray ionization


Food and Agriculture Organization of the United Nations


High-performance liquid chromatography–mass spectrometry


Hyperspectral imaging

IR light

Infrared light


Lettuce decay indices


Matrix-assisted laser desorption ionization


Mass spectrometry


Mass spectrometry imaging

NIR light

Near-infrared light


Nuclear magnetic resonance


Principal component analysis


Photomultiplier tube


Quantitative trait locus

RGB camera

Red–green–blue camera


Support vector data description

SWIR light

Short-wave infrared light

TIR light

Thermal infrared light


Total nitrogen content


Time-of-flight (mass spectrometer)



This work was supported by the research project of the Scientific Grant Agency of the Slovak Republic VEGA- 1-0923-16 and APVV-15-0721.

Competing Interests

The authors declare no financial conflict of interests.

Conflict of Interests

There are no conflicts of interest for this article.

Ethical Approval

The presented research does not require ethical approval.


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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Department of Plant PhysiologySlovak University of Agriculture in NitraNitraSlovak Republic
  2. 2.Plant Physiology and Ecology Department, Institute of BiologyTaras Shevchenko National University of KyivKyivUkraine
  3. 3.Slovak University of Agriculture in NitraNitraSlovak Republic

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