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Understanding Functional Genomics of PTGS Silencing Mechanisms for Tobacco Streak Virus and Other Ilarviruses Mediated by RNAi and VIGS

  • Avinash Marwal
  • R. K. GaurEmail author
Chapter

Abstract

Post-transcriptional gene silencing (PTGS) is a successful technology for the investigation of functions of gene in plants. In general, this phrase refers to the capability of a cell to avert the expression of a definite gene. PTGS can be achieved either by RNA interference (RNAi) or virus-induced gene silencing (VIGS). Tobacco Streak Virus (genus Ilarvirus and family Bromoviridae) consists of a tripartite genome and infects plants by causing symptoms like necrosis and leaf puckering. Ilarvirus are the most imperative viruses, thus causing enormous economic losses worldwide by plummeting crop production by its quantity and quality. Virus infection in plants is known to activate the silencing pathway in which siRNAs are produced. There are numerous reports for the genus Ilarvirus, which have confirmed that RNAi is engineered to target viral RNA in plants. RNA silencing is a high-throughput tool for restraining gene expression carried out by sequence-specific manner, chiefly via transcriptional repression or RNA degradation. As a retort to this defence mechanism, many ilarviruses programme gene silencing suppressor proteins performing at diverse stages in the silencing pathway.

Keywords

PTGS RNAi VIGS Tobacco Streak Virus Ilarvirus Silencing 

Notes

Acknowledgements

The authors are thankful to the Science and Engineering Research Board – Department of Science and Technology, New Delhi, India, for the financial assistance (File No. YSS/2015/000265) and also to the University Grants Commission, New Delhi, for providing financial assistantship under Research Award for Teacher (F.30-1/2014/RA-2014-16-GE-RAJ-4696 (SA-II).

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Copyright information

© Springer Nature Singapore Pte Ltd. 2017

Authors and Affiliations

  1. 1.Department of Biosciences, College of Arts, Science and HumanitiesMody UniversityLakshmangarh, SikarIndia

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