Prediction Expression Analysis and Target Validation of Bacterial Small RNAs
In the past few years, researchers from all over the world have unveiled a large number of small noncoding RNAs (sRNAs) in a wide variety of bacterial species. They play a central role in gene expression regulation of critical cellular processes in response to environmental changes. Most of them have been identified by predictive computational searches, while some have been detected through experimental approaches. The chapter reviews different computational studies available for predictive searches and various experimental strategies that have contributed for the detection and validation of sRNAs and their targets like microarrays, next-generation sequencing, and co-immunoprecipitation with Hfq. Although these approaches have led to the discovery of numerous sRNAs, regulatory roles of many of them are yet to be investigated.
This work was supported by grant from the DBT-Punjabi University Interdisciplinary Life Science Programme for advanced research and education (DBT-IPLS Project) No. BT/PR-4548/INF/22/146/2012.
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