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Enzymes as Molecular Tools

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Bioresources and Bioprocess in Biotechnology

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Abstract

Development of molecular techniques has led to revolutionary advancements in various fields of biosciences. Enzymes that modify nucleic acids are of paramount importance in these techniques. A wide range of enzymes involved in the catalysis of polymerization, ligation, cleavage, and other manipulations of DNA and RNA are currently available. Novel enzymes with improved properties are being developed by manufacturers. Molecular cloning is now a regular laboratory technique with a vast array of enzymes available from various commercial sources and to select an enzyme apt for a particular application has become a tedious task. This chapter aims to provide details on the different commercial enzymes available for routine molecular biology work, in addition to reviewing the important classes of enzymes that are used in molecular biology.

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Authors and Affiliations

  1. Division of Microbiology, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram, Kerala, India, 695562

    Gayathri Valsala & Shiburaj Sugathan

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  1. Gayathri Valsala
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  2. Shiburaj Sugathan
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Correspondence to Shiburaj Sugathan .

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Editors and Affiliations

  1. Microbiology Division, Jawaharlal Nehru Tropical Botanic Garden, Thiruvananthapuram, Kerala, India

    Shiburaj Sugathan

  2. Microbiology Division, Jawaharlal Nehru Tropical Botanic Garden, Thiruvananthapuram, Kerala, India

    N. S. Pradeep

  3. Inter University Centre for Bioscience, Department of Biotechnology and Microbiology, School of Life Sciences, Kannur University, Kannur, Kerala, India

    Sabu Abdulhameed

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Valsala, G., Sugathan, S. (2017). Enzymes as Molecular Tools. In: Sugathan, S., Pradeep, N., Abdulhameed, S. (eds) Bioresources and Bioprocess in Biotechnology. Springer, Singapore. https://doi.org/10.1007/978-981-10-4284-3_4

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