Propagation and Bioreactor Technology of Medicinal Plants: Case Studies on Paclitaxel, 10-Deacetylbaccatin III, and Camptothecin

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Abstract

The article consists of the studies carried out in the Forest Research Institute, Taiwan, for the production of commercially high-value compounds such as paclitaxel, 10-deacetylbaccatin III (10-DAB), and camptothecin (CPT). Two strategies were followed to produce these compounds: by cultivation of plants in a farm and by cell and hairy root cultures in suitable bioreactors. Both strategies essentially need a selection of target compounds and elite materials from natural genetic resources followed by optimization of efficient cultivation practices in a farm and establishment of an efficient in vitro propagation system by application of biotechnological tools including bioreactors. The production of medicinal plants in a farm is easier to farmers in contrast to bioreactors which often require high investment, carry high risk, and pose greater technical barriers. It was found that cell or hairy root cultures of Taxus, Camptotheca, and Nothapodytes grow well in wave bioreactor and thus may be a better approach for small and medium drug enterprises in Taiwan.

Keywords

Bioreactors Camptothecin 10-Deacetylbaccatin III Medicinal plants Propagation systems Paclitaxel 

Abbreviations

2,4-D

2,4-Dichlorophenoxyacetic acid

10-DAB

10-Deacetylbaccatin III

BA

6-Benzyladenine

cGMP

Current good manufacturing practice

CPT

Camptothecin

DBAT

10-Deacetylbaccatin III-10-O-acetyltransferase

DBH

Diameter at breath height

FDA

Food and Drug Administration

HCl

Hydrogen chloride

IAA

Indole-3-acetic acid

IBA

Indole-3-butyric acid

MJ

Methyl jasmonate

MS medium

Murashige and Skoog medium

NAA

1-Naphthaleneacetic acid

PVP

Polyvinylpyrrolidone

TDZ

Thidiazuron (N-phenyl-N′-1, 2, 3-thiadiazol-5-ylurea)

WPM

Woody plant medium

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Copyright information

© Springer Science+Business Media Singapore 2016

Authors and Affiliations

  1. 1.Taiwan Forestry Research InstituteTaipeiRepublic of China

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