Telocytes pp 325-334

Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 913) | Cite as

The Third Dimension of Telocytes Revealed by FIB-SEM Tomography

Chapter

Abstract

Lately, spatial three-dimensional (3D) identity of cells and their interrelations with the environment that surrounds it represent a challenging trend with the purpose to achieve a holistic view over the functions. Combining data from different imaging of cells in the third dimension can offer insight into behavior modalities making a world of difference. This chapter outlines a breakthrough in telocyte research by volume electron microscopy with the aid of focused ion beam scanning electron microscopy (FIB-SEM). Reconstructing 3D (three-dimensional) appearance of telocytes from a set of two-dimensional (2D) images by FIB-SEM tomography allowed to extract valuable data about their volume in nanoscale dimensions such as the three-dimensional morphology of telopodes and extracellular vesicles.

Keywords

FIB SEM tomography Telocytes Telopodes Extracellular vesicles 

References

  1. 1.
    Popescu LM, Faussone-Pellegrini MS. TELOCYTES – a case of serendipity: the winding way from Interstitial Cells of Cajal (ICC), via Interstitial Cajal-Like Cells (ICLC) to TELOCYTES. J Cell Mol Med. 2010;14:729–40.CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Cantarero I, Luesma MJ, Alvarez-Dotu JM, Munoz E, Junquera C. Transmission electron microscopy as key technique for the characterization of telocytes. Curr Stem Cell Res Ther. 2015;11:410–4.Google Scholar
  3. 3.
    Faussone Pellegrini MS, Popescu LM. Telocytes. Biomol Concepts. 2011;2:481–9.PubMedGoogle Scholar
  4. 4.
    Roatesi I, Radu BM, Cretoiu D, Cretoiu SM. Uterine telocytes: a review of current knowledge. Biol Reprod. 2015;93:10.CrossRefPubMedGoogle Scholar
  5. 5.
    Gherghiceanu M, Popescu LM. Cardiac telocytes – their junctions and functional implications. Cell Tissue Res. 2012;348:265–79.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Cretoiu SM, Cretoiu D, Marin A, Radu BM, Popescu LM. Telocytes: ultrastructural, immunohistochemical and electrophysiological characteristics in human myometrium. Reproduction. 2013;145:357–70.CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Fertig ET, Gherghiceanu M, Popescu LM. Extracellular vesicles release by cardiac telocytes: electron microscopy and electron tomography. J Cell Mol Med. 2014;18:1938–43.CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Popescu LM, Fertig ET, Gherghiceanu M. Reaching out: junctions between cardiac telocytes and cardiac stem cells in culture. J Cell Mol Med. 2016;20:370–80.Google Scholar
  9. 9.
    Sun X, Zheng M, Zhang M, Qian M, Zheng Y, Li M, et al. Differences in the expression of chromosome 1 genes between lung telocytes and other cells: mesenchymal stem cells, fibroblasts, alveolar type II cells, airway epithelial cells and lymphocytes. J Cell Mol Med. 2014;18:801–10.CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Zheng M, Sun X, Zhang M, Qian M, Zheng Y, Li M, et al. Variations of chromosomes 2 and 3 gene expression profiles among pulmonary telocytes, pneumocytes, airway cells, mesenchymal stem cells and lymphocytes. J Cell Mol Med. 2014;18:2044–60.CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Song D, Cretoiu D, Zheng M, Qian M, Zhang M, Cretoiu SM, et al. Comparison of Chromosome 4 gene expression profile between lung telocytes and other local cell types. J Cell Mol Med. 2016;20:71–80.Google Scholar
  12. 12.
    Liu J, Cao Y, Song Y, Huang Q, Wang F, Yang W, et al. Telocytes in liver. Curr Stem Cell Res Ther. 2016;11:415–9.Google Scholar
  13. 13.
    Zhu Y, Zheng M, Song D, Ye L, Wang X. Global comparison of chromosome X genes of pulmonary telocytes with mesenchymal stem cells, fibroblasts, alveolar type II cells, airway epithelial cells, and lymphocytes. J Transl Med. 2015;13:318.CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Cismasiu VB, Radu E, Popescu LM. miR-193 expression differentiates telocytes from other stromal cells. J Cell Mol Med. 2011;15:1071–4.CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Zheng Y, Cretoiu D, Yan G, Cretoiu SM, Popescu LM, Wang X. Comparative proteomic analysis of human lung telocytes with fibroblasts. J Cell Mol Med. 2014;18:568–89.CrossRefPubMedPubMedCentralGoogle Scholar
  16. 16.
    Zheng Y, Cretoiu D, Yan G, Cretoiu SM, Popescu LM, Fang H, et al. Protein profiling of human lung telocytes and microvascular endothelial cells using iTRAQ quantitative proteomics. J Cell Mol Med. 2014;18:1035–59.CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Albulescu R, Tanase C, Codrici E, Popescu DI, Cretoiu SM, Popescu LM. The secretome of myocardial telocytes modulates the activity of cardiac stem cells. J Cell Mol Med. 2015;19:1783–94.CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Cretoiu SM, Radu BM, Banciu A, Banciu DD, Cretoiu D, Ceafalan LC, et al. Isolated human uterine telocytes: immunocytochemistry and electrophysiology of T-type calcium channels. Histochem Cell Biol. 2015;143:83–94.CrossRefPubMedGoogle Scholar
  19. 19.
    Campeanu RA, Radu BM, Cretoiu SM, Banciu DD, Banciu A, Cretoiu D, et al. Near-infrared low-level laser stimulation of telocytes from human myometrium. Lasers Med Sci. 2014;29:1867–74.CrossRefPubMedPubMedCentralGoogle Scholar
  20. 20.
    Cretoiu SM, Popescu LM. Telocytes revisited. Biomol Concepts. 2014;5:353–69.CrossRefPubMedGoogle Scholar
  21. 21.
    Bei Y, Wang F, Yang C, Xiao J. Telocytes in regenerative medicine. J Cell Mol Med. 2015;19:1441–54.CrossRefPubMedPubMedCentralGoogle Scholar
  22. 22.
    Vannucchi MG, Bani D, Faussone-Pellegrini MS. Telocytes contribute as cell progenitors and differentiation inductors in tissue regeneration. Curr Stem Cell Res Ther. 2016;11:383–9.Google Scholar
  23. 23.
    Popescu LM, Gherghiceanu M, Cretoiu D, Radu E. The connective connection: interstitial cells of Cajal (ICC) and ICC-like cells establish synapses with immunoreactive cells. Electron microscope study in situ. J Cell Mol Med. 2005;9:714–30.CrossRefPubMedGoogle Scholar
  24. 24.
    Cretoiu D, Cretoiu SM, Simionescu AA, Popescu LM. Telocytes, a distinct type of cell among the stromal cells present in the lamina propria of jejunum. Histol Histopathol. 2012;27:1067–78.PubMedGoogle Scholar
  25. 25.
    Dawidowicz J, Szotek S, Matysiak N, Mielanczyk L, Maksymowicz K. Electron microscopy of human fascia lata: focus on telocytes. J Cell Mol Med. 2015;19:2500–6.CrossRefPubMedPubMedCentralGoogle Scholar
  26. 26.
    Cretoiu SM, Cretoiu D, Popescu LM. Human myometrium – the ultrastructural 3D network of telocytes. J Cell Mol Med. 2012;16:2844–9.CrossRefPubMedPubMedCentralGoogle Scholar
  27. 27.
    Peddie CJ, Collinson LM. Exploring the third dimension: volume electron microscopy comes of age. Micron. 2014;61:9–19.CrossRefPubMedGoogle Scholar
  28. 28.
    Kizilyaprak C, Daraspe J, Humbel BM. Focused ion beam scanning electron microscopy in biology. J Microsc. 2014;254:109–14.CrossRefPubMedGoogle Scholar
  29. 29.
    Narayan K, Danielson CM, Lagarec K, Lowekamp BC, Coffman P, Laquerre A, et al. Multi-resolution correlative focused ion beam scanning electron microscopy: applications to cell biology. J Struct Biol. 2014;185:278–84.CrossRefPubMedGoogle Scholar
  30. 30.
    Cretoiu D, Hummel E, Zimmermann H, Gherghiceanu M, Popescu LM. Human cardiac telocytes: 3D imaging by FIB-SEM tomography. J Cell Mol Med. 2014;18:2157–64.CrossRefPubMedPubMedCentralGoogle Scholar
  31. 31.
    Cretoiu D, Gherghiceanu M, Hummel E, Zimmermann H, Simionescu O, Popescu LM. FIB-SEM tomography of human skin telocytes and their extracellular vesicles. J Cell Mol Med. 2015;19:714–22.CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media Singapore 2016

Authors and Affiliations

  1. 1.Victor Babes National Institute of PathologyBucharestRomania
  2. 2.Division of Cellular and Molecular Biology and HistologyCarol Davila University of Medicine and PharmacyBucharestRomania

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