Assessment of Genetic Diversity in Indigenous Plants from Northeast India Using Molecular Marker Technology

Chapter

Abstract

Seven sister states of Northeast India possess a land area of about 262,000 km2, which is a home for diverse and unique natural resources. With 43 % plant diversity in this region, it can be called as a ‘biodiversity hotspot’ of India. However, this vast resource is being reduced due to heavy exploitation of forest land by expanding human population. Also climate changes are affecting the genetic diversity in this region. Therefore, there is an urgent need to formulate strategy and actions for conservation of natural resources for the sustainable development of this region. Presently, the researchers from various institutes and organizations are giving more emphasis on the development of molecular markers for various applications towards crop improvement. This chapter attempts to present an overview of use of different molecular markers-restriction fragment length polymorphisms (RFLPs), random amplified polymorphic DNAs (RAPDs), amplified fragment length polymorphisms (AFLPs), directed amplification of minisatellite DNA (DAMD), inter-simple sequence repeats (ISSR) and simple sequence microsatellites (SSR), which are currently available for studying genetic diversity. Prospects of application of some recent and advanced molecular markers-expressed sequence tags (ESTs) and single-nucleotide polymorphisms (SNPs) are also discussed.

Keywords

Genetic diversity Genetic markers PCR-based markers Northeast India 

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Copyright information

© Springer Science+Business Media Singapore 2016

Authors and Affiliations

  1. 1.Amity Institute of NanotechnologyAmity University Uttar PradeshNoidaIndia
  2. 2.O/o Directorate General of Life SciencesDefence Research and Development OrganizationNew DelhiIndia

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