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Methods That Make Your Cloning Life Easier

  • Seok-Yong Choi
  • Hyunju Ro
  • Hankuil Yi
Chapter

Abstract

In a modern biology lab, subcloning a gene obtained by RT-PCR into an expression vector and then expressing it in mammalian cells or E. coli are routinely performed. Sometimes, a cloned gene is directly inserted into an expression vector. Other times, it is inserted into a small vector with MCS and then transferred to an expression vector.

References

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Seok-Yong Choi
    • 1
  • Hyunju Ro
    • 2
  • Hankuil Yi
    • 3
  1. 1.Department of Biomedical SciencesChonnam National University Medical SchoolGwangjuSouth Korea
  2. 2.Department of Biological Sciences and BiotechnologyChungnam National UniversityDaejeonSouth Korea
  3. 3.Department of Biological SciencesChungnam National UniversityDaejeonSouth Korea

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