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The First Step in Cloning

  • Seok-Yong Choi
  • Hyunju Ro
  • Hankuil Yi
Chapter

Abstract

This is the simplest cloning strategy, which uses two RE sites in the MCS of a vector. Below are the step by step descriptions of the cut and paste method (Fig. 3.1).

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Seok-Yong Choi
    • 1
  • Hyunju Ro
    • 2
  • Hankuil Yi
    • 3
  1. 1.Department of Biomedical SciencesChonnam National University Medical SchoolGwangjuSouth Korea
  2. 2.Department of Biological Sciences and BiotechnologyChungnam National UniversityDaejeonSouth Korea
  3. 3.Department of Biological SciencesChungnam National UniversityDaejeonSouth Korea

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